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Literature summary extracted from

  • Vrielink, A.; Ghisla, S.
    Cholesterol oxidase: biochemistry and structural features (2009), FEBS J., 276, 6826-6843.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.6 0.04
-
 cholesterol substrate cholesterol, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 Brevibacterium sterolicum
1.1.3.6 0.07
-
 cholesterol substrate cholesterol, detection of product formation (cholest-4-en-3-one) at 240 nm. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 Brevibacterium sterolicum
1.1.3.6 0.11
-
 cholesterol substrate cholesterol, polarographic determination of the rate of oxygen consumption. 0.5 M potassium phosphate, 1% thesit (polyoxyethylene(9)-lauryl-ether), 1.25% propan-2-ol, 25°C and pH 7.5 Brevibacterium sterolicum
1.1.3.6 0.14
-
 cholesterol substrate cholesterol, detection of product formation (cholest-4-en-3-one) at 240 nm. 0.5 M potassium phosphate, 1% thesit (polyoxyethylene(9)-lauryl-ether), 1.25% propan-2-ol, 25°C and pH 7.5 Brevibacterium sterolicum
1.1.3.6 0.17
-
 cholesterol substrate cholesterol, polarographic determination of the rate of oxygen consumption. 1% thesit (polyoxyethylene(9)-lauryl-ether), 10% propan-2-ol and 50 mM phosphate, 25°C and pH 7.5 Streptomyces hygroscopicus
1.1.3.6 0.2
-
 cholestanol substrate cholestanol, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 Brevibacterium sterolicum
1.1.3.6 0.2
-
 cholesterol substrate cholesterol, detection of product formation (cholest-4-en-3-one) at 240 nm. 0.5 M potassium phosphate, 1% thesit (polyoxyethylene(9)-lauryl-ether), 1.25% propan-2-ol, 25°C and pH 7.5 Streptomyces hygroscopicus
1.1.3.6 0.2
-
 cholesterol substrate cholesterol, polarographic determination of the rate of oxygen consumption. 1% thesit (polyoxyethylene(9)-lauryl-ether), 10% propan-2-ol and 50 mM phosphate, 25°C and pH 7.5 Brevibacterium sterolicum
1.1.3.6 0.2
-
 pregnenolone substrate pregnenolone, detection of product formation at 240 nm. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 Streptomyces hygroscopicus
1.1.3.6 0.2
-
 pregnenolone substrate pregnenolone, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 Streptomyces hygroscopicus
1.1.3.6 0.2
-
 pregnenolone substrate pregnenolone, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 Brevibacterium sterolicum
1.1.3.6 0.2
-
 trans-dehydroandrosterone substrate trans-dehydroandrosterone, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 Streptomyces hygroscopicus
1.1.3.6 0.25
-
 cholesterol substrate cholesterol, polarographic determination of the rate of oxygen consumption. 0.5 M potassium phosphate, 1% thesit (polyoxyethylene(9)-lauryl-ether), 1.25% propan-2-ol, 25°C and pH 7.5 Streptomyces hygroscopicus
1.1.3.6 0.25
-
 cholesterol substrate cholesterol, polarographic determination of the rate of oxygen consumption. 1% thesit (polyoxyethylene(9)-lauryl-ether), 10% propan-2-ol and 50 mM phosphate, 25°C and pH 7.5 Brevibacterium sterolicum
1.1.3.6 0.3
-
 trans-dehydroandrosterone substrate trans-dehydroandrosterone, detection of product formation at 240 nm. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 Streptomyces hygroscopicus
1.1.3.6 0.4
-
 cholesterol substrate cholesterol, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 Streptomyces hygroscopicus
1.1.3.6 0.4
-
 pregnenolone substrate pregnenolone, detection of product formation at 240 nm. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 Brevibacterium sterolicum
1.1.3.6 0.5
-
 trans-androsterone substrate trans-androsterone, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 Streptomyces hygroscopicus
1.1.3.6 0.7
-
 cholestanol substrate cholestanol, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 Streptomyces hygroscopicus
1.1.3.6 0.8
-
 cholesterol substrate cholesterol, detection of product formation (cholest-4-en-3-one) at 240 nm. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 Streptomyces hygroscopicus
1.1.3.6 0.8
-
 trans-androsterone substrate trans-androsterone, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 Brevibacterium sterolicum
1.1.3.6 0.9
-
 trans-dehydroandrosterone substrate trans-dehydroandrosterone, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 Brevibacterium sterolicum
1.1.3.6 1.2
-
 trans-dehydroandrosterone substrate trans-dehydroandrosterone, detection of product formation at 240 nm. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 Brevibacterium sterolicum

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.6 Brevibacterium sterolicum
-
-
-
1.1.3.6 Streptomyces hygroscopicus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.6 cholestanol + O2
-
 Streptomyces hygroscopicus ?
-
 ?
1.1.3.6 cholestanol + O2
-
 Brevibacterium sterolicum ?
-
 ?
1.1.3.6 cholesterol + O2
-
 Streptomyces hygroscopicus cholest-4-en-3-one + H2O2
-
 ?
1.1.3.6 cholesterol + O2
-
 Brevibacterium sterolicum cholest-4-en-3-one + H2O2
-
 ?
1.1.3.6 pregnenolone + O2
-
 Streptomyces hygroscopicus ?
-
 ?
1.1.3.6 pregnenolone + O2
-
 Brevibacterium sterolicum ?
-
 ?
1.1.3.6 trans-androsterone + O2
-
 Streptomyces hygroscopicus ?
-
 ?
1.1.3.6 trans-androsterone + O2
-
 Brevibacterium sterolicum ?
-
 ?
1.1.3.6 trans-dehydroandrosterone + O2
-
 Streptomyces hygroscopicus ?
-
 ?
1.1.3.6 trans-dehydroandrosterone + O2
-
 Brevibacterium sterolicum ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.3.6 BsChOx
-
 Brevibacterium sterolicum
1.1.3.6 cholesterol oxidase
-
 Streptomyces hygroscopicus
1.1.3.6 cholesterol oxidase
-
 Brevibacterium sterolicum
1.1.3.6 ShChOx
-
 Streptomyces hygroscopicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.3.6 0.8
-
 trans-androsterone substrate trans-androsterone, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 Brevibacterium sterolicum
1.1.3.6 0.8
-
 trans-dehydroandrosterone substrate trans-dehydroandrosterone, detection of product formation at 240 nm. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 Brevibacterium sterolicum
1.1.3.6 1
-
 trans-dehydroandrosterone substrate trans-dehydroandrosterone, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 Brevibacterium sterolicum
1.1.3.6 3
-
 cholesterol substrate cholesterol, polarographic determination of the rate of oxygen consumption. 1% thesit (polyoxyethylene(9)-lauryl-ether), 10% propan-2-ol and 50 mM phosphate, 25°C and pH 7.5 Streptomyces hygroscopicus
1.1.3.6 6
-
 cholesterol substrate cholesterol, polarographic determination of the rate of oxygen consumption. 1% thesit (polyoxyethylene(9)-lauryl-ether), 10% propan-2-ol and 50 mM phosphate, 25°C and pH 7.5 Streptomyces hygroscopicus
1.1.3.6 6
-
 trans-dehydroandrosterone substrate trans-dehydroandrosterone, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 Streptomyces hygroscopicus
1.1.3.6 7
-
 trans-androsterone substrate trans-androsterone, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 Streptomyces hygroscopicus
1.1.3.6 8.2
-
 trans-dehydroandrosterone substrate trans-dehydroandrosterone, detection of product formation at 240 nm. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 Streptomyces hygroscopicus
1.1.3.6 9
-
 cholesterol substrate cholesterol, polarographic determination of the rate of oxygen consumption. 0.5 M potassium phosphate, 1% thesit (polyoxyethylene(9)-lauryl-ether), 1.25% propan-2-ol, 25°C and pH 7.5 Streptomyces hygroscopicus
1.1.3.6 11
-
 cholesterol substrate cholesterol, detection of product formation (cholest-4-en-3-one) at 240 nm. 0.5 M potassium phosphate, 1% thesit (polyoxyethylene(9)-lauryl-ether), 1.25% propan-2-ol, 25°C and pH 7.5 Streptomyces hygroscopicus
1.1.3.6 21
-
 pregnenolone substrate pregnenolone, detection of product formation at 240 nm. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 Brevibacterium sterolicum
1.1.3.6 21
-
 pregnenolone substrate pregnenolone, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 Streptomyces hygroscopicus
1.1.3.6 24
-
 pregnenolone substrate pregnenolone, detection of product formation at 240 nm. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 Streptomyces hygroscopicus
1.1.3.6 32
-
 cholesterol substrate cholesterol, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 Streptomyces hygroscopicus
1.1.3.6 35
-
 pregnenolone substrate pregnenolone, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 Brevibacterium sterolicum
1.1.3.6 37
-
 cholestanol substrate cholestanol, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 Streptomyces hygroscopicus
1.1.3.6 40
-
 cholestanol substrate cholestanol, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 Brevibacterium sterolicum
1.1.3.6 43
-
 cholesterol substrate cholesterol, polarographic determination of the rate of oxygen consumption. 1% thesit (polyoxyethylene(9)-lauryl-ether), 10% propan-2-ol and 50 mM phosphate, 25°C and pH 7.5 Brevibacterium sterolicum
1.1.3.6 48
-
 cholesterol substrate cholesterol, detection of product formation (cholest-4-en-3-one) at 240 nm. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 Brevibacterium sterolicum
1.1.3.6 48
-
 cholesterol substrate cholesterol, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 Brevibacterium sterolicum
1.1.3.6 56
-
 cholesterol substrate cholesterol, polarographic determination of the rate of oxygen consumption. 0.5 M potassium phosphate, 1% thesit (polyoxyethylene(9)-lauryl-ether), 1.25% propan-2-ol, 25°C and pH 7.5 Brevibacterium sterolicum
1.1.3.6 57
-
 cholesterol substrate cholesterol, polarographic determination of the rate of oxygen consumption. 1% thesit (polyoxyethylene(9)-lauryl-ether), 10% propan-2-ol and 50 mM phosphate, 25°C and pH 7.5 Brevibacterium sterolicum
1.1.3.6 63
-
 cholesterol substrate cholesterol, detection of product formation (cholest-4-en-3-one) at 240 nm. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 Streptomyces hygroscopicus
1.1.3.6 67
-
 cholesterol substrate cholesterol, detection of product formation (cholest-4-en-3-one) at 240 nm. 0.5 M potassium phosphate, 1% thesit (polyoxyethylene(9)-lauryl-ether), 1.25% propan-2-ol, 25°C and pH 7.5 Brevibacterium sterolicum

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.6 FAD covalently linked Brevibacterium sterolicum
1.1.3.6 FAD noncovalently bound to the enzyme Streptomyces hygroscopicus