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Literature summary extracted from

  • Ilari, A.; Fiorillo, A.; Angelaccio, S.; Florio, R.; Chiaraluce, R.; van der Oost, J.; Consalvi, V.
    Crystal structure of a family 16 endoglucanase from the hyperthermophile Pyrococcus furiosus - structural basis of substrate recognition (2009), FEBS J., 276, 1048-1058.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.39 expression in Escherichia coli Pyrococcus furiosus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.39 hanging drop vapour diffusion technique at 20°C, crystal structure is determined at 2.1 A resolution by molecular replacement Pyrococcus furiosus
3.2.1.39 to 2.1 A resolution, determination by molecular replacement. Structure reveals a kink of six residues at the entrance of the catalytic cleft Pyrococcus furiosus

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.39 delI72-G75 deletion mutant, lacking residues 72-75, hydrolyses the mixed-linkage beta-1,3-1,4-glucan lichenan at 40°C and 70°C 10times more efficiently than the wiild-type protein. At 80°C, the specific activity of the D-loop mutant is reduced significantly relative to that of the wild-type with both laminarin and lichenan Pyrococcus furiosus
3.2.1.39 additional information deletion mutant lacking resiudes 72-75 hydrolyzes the mixed-linkgae beta-(1-3)-(1-4)-glucan lichenan 10times more efficiently than wild-type Pyrococcus furiosus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.39 Ca2+ one ion per monomer, crystallization data Pyrococcus furiosus
3.2.1.39 Ca2+ contains one calcium ion for each monomer. The calcium ion is located on the opposite side with respect to the catalytic cleft Pyrococcus furiosus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.39 Pyrococcus furiosus
-
-
-
3.2.1.39 Pyrococcus furiosus O73951
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.39 11.1
-
 wild-type, substrate lichenan, 40°C Pyrococcus furiosus
3.2.1.39 102.8
-
 mutant lacking residues 72-75, substrate lichenan, 80°C Pyrococcus furiosus
3.2.1.39 115.7
-
 mutant lacking residues 72-75, substrate lichenan, 40°C Pyrococcus furiosus
3.2.1.39 115.7
-
 wild-type, substrate lichenan, 70°C Pyrococcus furiosus
3.2.1.39 126.8
-
 wild-type, substrate laminarin, 40°C Pyrococcus furiosus
3.2.1.39 141.4
-
 mutant lacking residues 72-75, substrate laminarin, 40°C Pyrococcus furiosus
3.2.1.39 185
-
 wild-type, substrate lichenan, 80°C Pyrococcus furiosus
3.2.1.39 249
-
 mutant lacking residues 72-75, substrate laminarin, 80°C Pyrococcus furiosus
3.2.1.39 263.6
-
 mutant lacking residues 72-75, substrate lichenan, 70°C Pyrococcus furiosus
3.2.1.39 334.3
-
 mutant lacking residues 72-75, substrate laminarin, 70°C Pyrococcus furiosus
3.2.1.39 659.3
-
 wild-type, substrate laminarin, 70°C Pyrococcus furiosus
3.2.1.39 1087
-
 wild-type, substrate laminarin, 80°C Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.39 laminarin + H2O
-
 Pyrococcus furiosus ?
-
 ?
3.2.1.39 lichenan + H2O
-
 Pyrococcus furiosus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.39 PfLamA
-
 Pyrococcus furiosus