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Literature summary extracted from

  • Berrocal, M.; Marcos, D.; Sepulveda, M.R.; Perez, M.; Avila, J.; Mata, A.M.
    Altered Ca2+ dependence of synaptosomal plasma membrane Ca2+-ATPase in human brain affected by Alzheimers disease (2009), FASEB J., 23, 1826-1834.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
7.2.2.10 Calmodulin
-
 Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.2.2.10 expressed in HEK-293 cells Homo sapiens
7.2.2.10 expressed in HEK-293 cells Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
7.2.2.10 amyloid beta-peptide the addition of amyloid beta-peptide to normal brain decreases the PMCA activity resulting in the same Ca2+ dependency as that seen in Alzheimer's disease brain, whereas the addition of amyloid beta-peptide to Alzheimer's disease brain has no effect on PMCA activity, in the absence of cholesterol, the level of inhibition of cerebrum PMCA is 60%, but levels of inhibition decreases with increasing concentrations of cholesterol Homo sapiens
7.2.2.10 amyloid beta-peptide 1-40
-
 Sus scrofa
7.2.2.10 amyloid beta-peptide 25-35 a maximum inhibition of 75% is observed with 0.0025 mM amyloid beta-peptide 25-35 for PMCA reconstituted in phosphatidylcholine. Homo sapiens
7.2.2.10 amyloid beta-peptide 25-35 the activity of overexpressed PMCA4 in a microsomal fraction of the HEK-293 cells is inhibited by amyloid beta-peptide 25-35, a slight inhibition of the activity of PMCA3 is observed Sus scrofa
7.2.2.10 additional information no inhibition of isoforms PMCA4 and PMCA2 is observed with the amyloid beta-peptide 25-35. The addition of 0.0025 mM amyloid beta-peptide 25-35 to Alzheimer's disease brain has no effect on PMCA activity. No effect of amyloid beta-peptides is seen on SERCA or SPCA activity in either control or Alzheimer's disease brains Homo sapiens
7.2.2.10 additional information no inhibition of isoforms PMCA4 and PMCA2 is observed with the amyloid beta-peptide 25-35, 0.0025 mM amyloid beta-peptide 25-35 has no effect on the activity of PMCA purified from cerebellum Sus scrofa
7.2.2.10 thapsigargin inhibits SERCA activity Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.2.2.10 plasma membrane
-
 Homo sapiens 5886
-
7.2.2.10 plasma membrane
-
 Sus scrofa 5886
-
7.2.2.10 synaptosome
-
 Homo sapiens
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
7.2.2.10 Ca2+ dependent on Homo sapiens
7.2.2.10 Ca2+ dependent on Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
7.2.2.10 Homo sapiens
-
-
-
7.2.2.10 Sus scrofa P23220
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
7.2.2.10 brain
-
 Homo sapiens
-
7.2.2.10 cerebellum isoform PMCA 2 is more abundant in the cerebellum than in the cerebrum Sus scrofa
-
7.2.2.10 telencephalon isoform PMCA 4 is more abundant in the cerebrum than in the cerebellum Sus scrofa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.2.2.10 ATP + H2O + Ca2+/cis
-
 Homo sapiens ADP + phosphate + Ca2+/trans
-
 ?
7.2.2.10 ATP + H2O + Ca2+/cis
-
 Sus scrofa ADP + phosphate + Ca2+/trans
-
 ?

Synonyms

EC Number Synonyms Comment Organism
7.2.2.10 plasma membrane Ca2+-ATPase
-
 Homo sapiens
7.2.2.10 plasma membrane Ca2+-ATPase
-
 Sus scrofa
7.2.2.10 PMCA
-
 Homo sapiens
7.2.2.10 PMCA
-
 Sus scrofa
7.2.2.10 SERCA
-
 Homo sapiens
7.2.2.10 SPCA
-
 Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
7.2.2.10 0.0025
-
-
 Sus scrofa amyloid beta-peptide 25-35