BRENDA - Enzyme Database show

Molecular cloning and functional characterization of cynomolgus monkey multidrug resistance-associated protein 2 (MRP2)

Yasunaga, M.; Takemura, M.; Fujita, K.; Yabuuchi, H.; Wada, M.; Eur. J. Pharm. Sci. 35, 326-334 (2008)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
7.6.2.2
estrone glucuronide
the transport substrate stimulates the ATPase activity of MRP2
Macaca fascicularis
7.6.2.2
Estrone sulfate
the transport substrate stimulates the ATPase activity of MRP2
Macaca fascicularis
7.6.2.2
methotrexate
the transport substrate stimulates the ATPase activity of MRP2
Macaca fascicularis
7.6.2.2
probenecid
the transport substrate stimulates the ATPase activity of MRP2
Macaca fascicularis
7.6.2.2
vinblastine
the transport substrate stimulates the ATPase activity of MRP2
Macaca fascicularis
Cloned(Commentary)
EC Number
Commentary
Organism
7.6.2.2
MRP2, DNA and amino acid sequence determination and analysis, expression of MRP2 in Spodoptera frugiperda Sf9 cells
Macaca fascicularis
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
7.6.2.2
vanadate
inhibits the ATPase activity of MRP2
Macaca fascicularis
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
7.6.2.2
0.0069
-
leukotriene C4
pH 7.0, 37°C, recombinant MRP2
Macaca fascicularis
7.6.2.2
0.0108
-
vinblastine
pH 7.0, 37°C, recombinant MRP2
Macaca fascicularis
7.6.2.2
0.0109
-
leukotriene C4
pH 7.0, 37°C, recombinant MRP2
Homo sapiens
7.6.2.2
0.0115
-
2,4-dinitrophenyl-S-glutathione
pH 7.0, 37°C, recombinant MRP2
Macaca fascicularis
7.6.2.2
0.0263
-
Estrone 3-sulfate
pH 7.0, 37°C, recombinant MRP2
Macaca fascicularis
7.6.2.2
0.052
-
vinblastine
pH 7.0, 37°C, recombinant MRP2
Homo sapiens
7.6.2.2
0.075
-
17beta-estradiol 17-(beta-D-glucuronide)
pH 7.0, 37°C, recombinant MRP2
Macaca fascicularis
7.6.2.2
0.108
-
2,4-dinitrophenyl-S-glutathione
pH 7.0, 37°C, recombinant MRP2
Homo sapiens
7.6.2.2
0.134
-
Estrone 3-sulfate
pH 7.0, 37°C, recombinant MRP2
Homo sapiens
7.6.2.2
0.154
-
probenecid
pH 7.0, 37°C, recombinant MRP2
Macaca fascicularis
7.6.2.2
0.195
-
17beta-estradiol 17-(beta-D-glucuronide)
pH 7.0, 37°C, recombinant MRP2
Homo sapiens
7.6.2.2
0.282
-
methotrexate
pH 7.0, 37°C, recombinant MRP2
Macaca fascicularis
7.6.2.2
0.351
-
probenecid
pH 7.0, 37°C, recombinant MRP2
Homo sapiens
7.6.2.2
4.522
-
methotrexate
pH 7.0, 37°C, recombinant MRP2
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
7.6.2.2
membrane
-
Macaca fascicularis
16020
-
7.6.2.2
membrane
-
Homo sapiens
16020
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
7.6.2.2
Mg2+
required
Macaca fascicularis
7.6.2.2
Mg2+
required
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
7.6.2.2
ATP + H2O + xenobiotic/in
Homo sapiens
-
ADP + phosphate + xenobiotic/out
-
-
?
7.6.2.2
ATP + H2O + xenobiotic/in
Macaca fascicularis
-
ADP + phosphate + xenobiotic/out
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
7.6.2.2
Homo sapiens
-
-
-
7.6.2.2
Macaca fascicularis
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
7.6.2.2
kidney
high expression level of MRP2
Macaca fascicularis
-
7.6.2.2
additional information
tissue-specific expression of monkey MRP2, no expression in cerebrum and colon
Macaca fascicularis
-
7.6.2.2
small intestine
high expression level of MRP2
Macaca fascicularis
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
7.6.2.2
ATP + H2O + 17beta-estradiol 17-(beta-D-glucuronide)/in
-
697760
Homo sapiens
ADP + phosphate + 17beta-estradiol 17-(beta-D-glucuronide)/out
-
-
-
?
7.6.2.2
ATP + H2O + 17beta-estradiol 17-(beta-D-glucuronide)/in
-
697760
Macaca fascicularis
ADP + phosphate + 17beta-estradiol 17-(beta-D-glucuronide)/out
-
-
-
?
7.6.2.2
ATP + H2O + estrone-3-sulfate/in
-
697760
Homo sapiens
ADP + phosphate + estrone-3-sulfate/out
-
-
-
?
7.6.2.2
ATP + H2O + estrone-3-sulfate/in
-
697760
Macaca fascicularis
ADP + phosphate + estrone-3-sulfate/out
-
-
-
?
7.6.2.2
ATP + H2O + leukotriene C4/in
-
697760
Homo sapiens
ADP + phosphate + leukotriene C4/out
-
-
-
?
7.6.2.2
ATP + H2O + leukotriene C4/in
-
697760
Macaca fascicularis
ADP + phosphate + leukotriene C4/out
-
-
-
?
7.6.2.2
ATP + H2O + methotrexate/in
-
697760
Homo sapiens
ADP + phosphate + methotrexate/out
-
-
-
?
7.6.2.2
ATP + H2O + methotrexate/in
-
697760
Macaca fascicularis
ADP + phosphate + methotrexate/out
-
-
-
?
7.6.2.2
ATP + H2O + probenecid/in
-
697760
Homo sapiens
ADP + phosphate + probenecid/out
-
-
-
?
7.6.2.2
ATP + H2O + probenecid/in
-
697760
Macaca fascicularis
ADP + phosphate + probenecid/out
-
-
-
?
7.6.2.2
ATP + H2O + S-2,4-dinitrophenylglutathione/in
-
697760
Homo sapiens
ADP + phosphate + S-2,4-dinitrophenylglutathione/out
-
-
-
?
7.6.2.2
ATP + H2O + S-2,4-dinitrophenylglutathione/in
-
697760
Macaca fascicularis
ADP + phosphate + S-2,4-dinitrophenylglutathione/out
-
-
-
?
7.6.2.2
ATP + H2O + vinblastine/in
-
697760
Homo sapiens
ADP + phosphate + vinblastine/out
-
-
-
?
7.6.2.2
ATP + H2O + vinblastine/in
-
697760
Macaca fascicularis
ADP + phosphate + vinblastine/out
-
-
-
?
7.6.2.2
ATP + H2O + xenobiotic/in
-
697760
Homo sapiens
ADP + phosphate + xenobiotic/out
-
-
-
?
7.6.2.2
ATP + H2O + xenobiotic/in
-
697760
Macaca fascicularis
ADP + phosphate + xenobiotic/out
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
7.6.2.2
37
-
assay at
Homo sapiens
7.6.2.2
37
-
assay at
Macaca fascicularis
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.6.2.2
7
-
assay at
Homo sapiens
7.6.2.2
7
-
assay at
Macaca fascicularis
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
7.6.2.2
ATP
-
Macaca fascicularis
7.6.2.2
ATP
-
Homo sapiens
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
7.6.2.2
estrone glucuronide
the transport substrate stimulates the ATPase activity of MRP2
Macaca fascicularis
7.6.2.2
Estrone sulfate
the transport substrate stimulates the ATPase activity of MRP2
Macaca fascicularis
7.6.2.2
methotrexate
the transport substrate stimulates the ATPase activity of MRP2
Macaca fascicularis
7.6.2.2
probenecid
the transport substrate stimulates the ATPase activity of MRP2
Macaca fascicularis
7.6.2.2
vinblastine
the transport substrate stimulates the ATPase activity of MRP2
Macaca fascicularis
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
7.6.2.2
MRP2, DNA and amino acid sequence determination and analysis, expression of MRP2 in Spodoptera frugiperda Sf9 cells
Macaca fascicularis
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
7.6.2.2
ATP
-
Macaca fascicularis
7.6.2.2
ATP
-
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
7.6.2.2
vanadate
inhibits the ATPase activity of MRP2
Macaca fascicularis
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
7.6.2.2
0.0069
-
leukotriene C4
pH 7.0, 37°C, recombinant MRP2
Macaca fascicularis
7.6.2.2
0.0108
-
vinblastine
pH 7.0, 37°C, recombinant MRP2
Macaca fascicularis
7.6.2.2
0.0109
-
leukotriene C4
pH 7.0, 37°C, recombinant MRP2
Homo sapiens
7.6.2.2
0.0115
-
2,4-dinitrophenyl-S-glutathione
pH 7.0, 37°C, recombinant MRP2
Macaca fascicularis
7.6.2.2
0.0263
-
Estrone 3-sulfate
pH 7.0, 37°C, recombinant MRP2
Macaca fascicularis
7.6.2.2
0.052
-
vinblastine
pH 7.0, 37°C, recombinant MRP2
Homo sapiens
7.6.2.2
0.075
-
17beta-estradiol 17-(beta-D-glucuronide)
pH 7.0, 37°C, recombinant MRP2
Macaca fascicularis
7.6.2.2
0.108
-
2,4-dinitrophenyl-S-glutathione
pH 7.0, 37°C, recombinant MRP2
Homo sapiens
7.6.2.2
0.134
-
Estrone 3-sulfate
pH 7.0, 37°C, recombinant MRP2
Homo sapiens
7.6.2.2
0.154
-
probenecid
pH 7.0, 37°C, recombinant MRP2
Macaca fascicularis
7.6.2.2
0.195
-
17beta-estradiol 17-(beta-D-glucuronide)
pH 7.0, 37°C, recombinant MRP2
Homo sapiens
7.6.2.2
0.282
-
methotrexate
pH 7.0, 37°C, recombinant MRP2
Macaca fascicularis
7.6.2.2
0.351
-
probenecid
pH 7.0, 37°C, recombinant MRP2
Homo sapiens
7.6.2.2
4.522
-
methotrexate
pH 7.0, 37°C, recombinant MRP2
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
7.6.2.2
membrane
-
Macaca fascicularis
16020
-
7.6.2.2
membrane
-
Homo sapiens
16020
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
7.6.2.2
Mg2+
required
Macaca fascicularis
7.6.2.2
Mg2+
required
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
7.6.2.2
ATP + H2O + xenobiotic/in
Homo sapiens
-
ADP + phosphate + xenobiotic/out
-
-
?
7.6.2.2
ATP + H2O + xenobiotic/in
Macaca fascicularis
-
ADP + phosphate + xenobiotic/out
-
-
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
7.6.2.2
kidney
high expression level of MRP2
Macaca fascicularis
-
7.6.2.2
additional information
tissue-specific expression of monkey MRP2, no expression in cerebrum and colon
Macaca fascicularis
-
7.6.2.2
small intestine
high expression level of MRP2
Macaca fascicularis
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
7.6.2.2
ATP + H2O + 17beta-estradiol 17-(beta-D-glucuronide)/in
-
697760
Homo sapiens
ADP + phosphate + 17beta-estradiol 17-(beta-D-glucuronide)/out
-
-
-
?
7.6.2.2
ATP + H2O + 17beta-estradiol 17-(beta-D-glucuronide)/in
-
697760
Macaca fascicularis
ADP + phosphate + 17beta-estradiol 17-(beta-D-glucuronide)/out
-
-
-
?
7.6.2.2
ATP + H2O + estrone-3-sulfate/in
-
697760
Homo sapiens
ADP + phosphate + estrone-3-sulfate/out
-
-
-
?
7.6.2.2
ATP + H2O + estrone-3-sulfate/in
-
697760
Macaca fascicularis
ADP + phosphate + estrone-3-sulfate/out
-
-
-
?
7.6.2.2
ATP + H2O + leukotriene C4/in
-
697760
Homo sapiens
ADP + phosphate + leukotriene C4/out
-
-
-
?
7.6.2.2
ATP + H2O + leukotriene C4/in
-
697760
Macaca fascicularis
ADP + phosphate + leukotriene C4/out
-
-
-
?
7.6.2.2
ATP + H2O + methotrexate/in
-
697760
Homo sapiens
ADP + phosphate + methotrexate/out
-
-
-
?
7.6.2.2
ATP + H2O + methotrexate/in
-
697760
Macaca fascicularis
ADP + phosphate + methotrexate/out
-
-
-
?
7.6.2.2
ATP + H2O + probenecid/in
-
697760
Homo sapiens
ADP + phosphate + probenecid/out
-
-
-
?
7.6.2.2
ATP + H2O + probenecid/in
-
697760
Macaca fascicularis
ADP + phosphate + probenecid/out
-
-
-
?
7.6.2.2
ATP + H2O + S-2,4-dinitrophenylglutathione/in
-
697760
Homo sapiens
ADP + phosphate + S-2,4-dinitrophenylglutathione/out
-
-
-
?
7.6.2.2
ATP + H2O + S-2,4-dinitrophenylglutathione/in
-
697760
Macaca fascicularis
ADP + phosphate + S-2,4-dinitrophenylglutathione/out
-
-
-
?
7.6.2.2
ATP + H2O + vinblastine/in
-
697760
Homo sapiens
ADP + phosphate + vinblastine/out
-
-
-
?
7.6.2.2
ATP + H2O + vinblastine/in
-
697760
Macaca fascicularis
ADP + phosphate + vinblastine/out
-
-
-
?
7.6.2.2
ATP + H2O + xenobiotic/in
-
697760
Homo sapiens
ADP + phosphate + xenobiotic/out
-
-
-
?
7.6.2.2
ATP + H2O + xenobiotic/in
-
697760
Macaca fascicularis
ADP + phosphate + xenobiotic/out
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
7.6.2.2
37
-
assay at
Homo sapiens
7.6.2.2
37
-
assay at
Macaca fascicularis
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.6.2.2
7
-
assay at
Homo sapiens
7.6.2.2
7
-
assay at
Macaca fascicularis