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Literature summary extracted from

  • Iobbi, C.; Santini, C.L.; Bonnefoy, V.; Giordano, G.
    Biochemical and immunological evidence for a second nitrate reductase in Escherichia coli K12 (1987), Eur. J. Biochem., 168, 451-459.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.5.1 KCN 1 mM, complete inhibition Escherichia coli K-12
1.7.5.1 NaN3 10 mM, complete inhibition Escherichia coli K-12

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.5.1 3.9
-
 nitrate cosubstrate: benzyl viologen Escherichia coli K-12

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.7.5.1 membrane
-
 Escherichia coli K-12 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.7.5.1 Mo molybdoenzyme Escherichia coli K-12

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.7.5.1 additional information Escherichia coli K-12 nitrate reductase Z is synthesized in small amounts, the expression of its structural genes does not seem to be induced by nitrate, repressed by oxygen or activated by the product of the fnr gene. The nitrate reductase Z in mutant LCB79/pLCB14 couples formate oxidation with nitrate reduction probably via quinones and type-b cytochromes ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.7.5.1 Escherichia coli K-12
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.5.1 additional information nitrate reductase Z is synthesized in small amounts, the expression of its structural genes does not seem to be induced by nitrate, repressed by oxygen or activated by the product of the fnr gene. The nitrate reductase Z in mutant LCB79/pLCB14 couples formate oxidation with nitrate reduction probably via quinones and type-b cytochromes Escherichia coli K-12 ?
-
 ?
1.7.5.1 additional information nitrate reductase Z is able to use both nitrate and chlorate as substrate Escherichia coli K-12 ?
-
 ?
1.7.5.1 nitrate + reduced benzyl viologen
-
 Escherichia coli K-12 nitrite + benzyl viologen
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.7.5.1 nitrate reductase Z
-
 Escherichia coli K-12

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.7.5.1 60
-
 20 min, stable Escherichia coli K-12
1.7.5.1 65
-
 4 min, 50% loss of activity Escherichia coli K-12

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.5.1 6.5
-
-
 Escherichia coli K-12