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Literature summary extracted from

  • Selinheimo, E.; Gasparetti, C.; Mattinen, M.; Steffensen, C.; Buchert, J.; Kruus, K.
    Comparison of substrate specificity of tyrosinases from Trichoderma reesei and Agaricus bisporus (2009), Enzyme Microb. Technol., 44, 1-10.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.18.1 0.06
-
 p-tyrosol in 0.1 M sodium phosphate buffer (pH 7.0) at 25°C Agaricus bisporus
1.14.18.1 0.11
-
 Gly-Gly-L-Tyr in 0.1 M sodium phosphate buffer (pH 7.0) at 25°C Agaricus bisporus
1.14.18.1 0.17
-
 L-Dopa in 0.1 M sodium phosphate buffer (pH 7.0) at 25°C Agaricus bisporus
1.14.18.1 0.18
-
 Gly-L-Tyr-Gly in 0.1 M sodium phosphate buffer (pH 7.0) at 25°C Agaricus bisporus
1.14.18.1 0.2
-
 L-tyrosine in 0.1 M sodium phosphate buffer (pH 7.0) at 25°C Agaricus bisporus
1.14.18.1 0.21
-
 L-Tyr-Gly-Gly in 0.1 M sodium phosphate buffer (pH 7.0) at 25°C Agaricus bisporus
1.14.18.1 0.25
-
 catechol in 0.1 M sodium phosphate buffer (pH 7.0) at 25°C Agaricus bisporus
1.14.18.1 0.33
-
 phenol in 0.1 M sodium phosphate buffer (pH 7.0) at 25°C Agaricus bisporus
1.14.18.1 0.64
-
 phloretic acid in 0.1 M sodium phosphate buffer (pH 7.0) at 25°C Agaricus bisporus
1.14.18.1 0.75
-
 tyramine in 0.1 M sodium phosphate buffer (pH 7.0) at 25°C Agaricus bisporus
1.14.18.1 0.8
-
 p-tyrosol in 0.1M sodium phosphate buffer (pH 7.0) at 25°C Trichoderma reesei
1.14.18.1 0.84
-
 dopamine in 0.1 M sodium phosphate buffer (pH 7.0) at 25°C Agaricus bisporus
1.14.18.1 0.9
-
 caffeic acid in 0.1M sodium phosphate buffer (pH 7.0) at 25°C Trichoderma reesei
1.14.18.1 0.91
-
 Hydrocaffeic acid in 0.1 M sodium phosphate buffer (pH 7.0) at 25°C Agaricus bisporus
1.14.18.1 1.4
-
 phloretic acid in 0.1M sodium phosphate buffer (pH 7.0) at 25°C Trichoderma reesei
1.14.18.1 1.5
-
 p-coumaric acid in 0.1M sodium phosphate buffer (pH 7.0) at 25°C Trichoderma reesei
1.14.18.1 1.69
-
 caffeic acid in 0.1 M sodium phosphate buffer (pH 7.0) at 25°C Agaricus bisporus
1.14.18.1 2.5
-
 catechol in 0.1M sodium phosphate buffer (pH 7.0) at 25°C Trichoderma reesei
1.14.18.1 3
-
 Hydrocaffeic acid in 0.1M sodium phosphate buffer (pH 7.0) at 25°C Trichoderma reesei
1.14.18.1 3.1
-
 Gly-Gly-L-Tyr in 0.1M sodium phosphate buffer (pH 7.0) at 25°C Trichoderma reesei
1.14.18.1 3.8
-
 phenol in 0.1M sodium phosphate buffer (pH 7.0) at 25°C Trichoderma reesei
1.14.18.1 3.9
-
 Gly-L-Tyr-Gly in 0.1M sodium phosphate buffer (pH 7.0) at 25°C Trichoderma reesei
1.14.18.1 4.5
-
 tyramine in 0.1M sodium phosphate buffer (pH 7.0) at 25°C Trichoderma reesei
1.14.18.1 6
-
 L-Tyr-Gly-Gly in 0.1M sodium phosphate buffer (pH 7.0) at 25°C Trichoderma reesei
1.14.18.1 7.5
-
 L-Dopa in 0.1M sodium phosphate buffer (pH 7.0) at 25°C Trichoderma reesei
1.14.18.1 11
-
 dopamine in 0.1M sodium phosphate buffer (pH 7.0) at 25°C Trichoderma reesei

Organism

EC Number Organism UniProt Comment Textmining
1.14.18.1 Agaricus bisporus
-
-
-
1.14.18.1 Trichoderma reesei
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.18.1 2 catechol + O2
-
 Agaricus bisporus 2 1,2-benzoquinone + 2 H2O
-
 ?
1.14.18.1 2 catechol + O2
-
 Trichoderma reesei 2 1,2-benzoquinone + 2 H2O
-
 ?
1.14.18.1 caffeic acid + O2
-
 Agaricus bisporus caffeoyl quinone + H2O
-
 ?
1.14.18.1 caffeic acid + O2
-
 Trichoderma reesei caffeoyl quinone + H2O
-
 ?
1.14.18.1 dopamine + O2
-
 Agaricus bisporus ?
-
 ?
1.14.18.1 dopamine + O2
-
 Trichoderma reesei ?
-
 ?
1.14.18.1 Gly-Gly-L-Tyr + O2
-
 Agaricus bisporus ?
-
 ?
1.14.18.1 Gly-Gly-L-Tyr + O2
-
 Trichoderma reesei ?
-
 ?
1.14.18.1 Gly-L-Tyr-Gly + O2
-
 Agaricus bisporus ?
-
 ?
1.14.18.1 Gly-L-Tyr-Gly + O2
-
 Trichoderma reesei ?
-
 ?
1.14.18.1 hydrocaffeic acid + O2
-
 Agaricus bisporus ?
-
 ?
1.14.18.1 hydrocaffeic acid + O2
-
 Trichoderma reesei ?
-
 ?
1.14.18.1 L-DOPA + O2
-
 Agaricus bisporus dopaquinone + H2O
-
 ?
1.14.18.1 L-DOPA + O2
-
 Trichoderma reesei dopaquinone + H2O
-
 ?
1.14.18.1 L-Tyr-Gly-Gly + O2
-
 Agaricus bisporus ?
-
 ?
1.14.18.1 L-Tyr-Gly-Gly + O2
-
 Trichoderma reesei ?
-
 ?
1.14.18.1 L-tyrosine + O2
-
 Agaricus bisporus L-DOPA + H2O
-
 ?
1.14.18.1 L-tyrosine + O2
-
 Trichoderma reesei L-DOPA + H2O
-
 ?
1.14.18.1 p-coumaric acid + O2
-
 Agaricus bisporus caffeic acid + H2O
-
 ?
1.14.18.1 p-coumaric acid + O2
-
 Trichoderma reesei caffeic acid + H2O
-
 ?
1.14.18.1 p-tyrosol + O2
-
 Agaricus bisporus 2-(3,4-dihydroxyphenyl)ethanol + H2O
-
 ?
1.14.18.1 p-tyrosol + O2
-
 Trichoderma reesei 2-(3,4-dihydroxyphenyl)ethanol + H2O
-
 ?
1.14.18.1 phenol + O2
-
 Agaricus bisporus ?
-
 ?
1.14.18.1 phenol + O2
-
 Trichoderma reesei ?
-
 ?
1.14.18.1 phloretic acid + O2
-
 Agaricus bisporus ?
-
 ?
1.14.18.1 phloretic acid + O2
-
 Trichoderma reesei ?
-
 ?
1.14.18.1 tyramine + O2
-
 Agaricus bisporus ?
-
 ?
1.14.18.1 tyramine + O2
-
 Trichoderma reesei ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.18.1 monophenol, o-diphenol:oxygen oxidoreductase
-
 Agaricus bisporus
1.14.18.1 monophenol, o-diphenol:oxygen oxidoreductase
-
 Trichoderma reesei
1.14.18.1 tyrosinase
-
 Agaricus bisporus
1.14.18.1 tyrosinase
-
 Trichoderma reesei

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.18.1 3
-
 Gly-Gly-L-Tyr in 0.1M sodium phosphate buffer (pH 7.0) at 25°C Agaricus bisporus
1.14.18.1 3.1
-
 Gly-L-Tyr-Gly in 0.1M sodium phosphate buffer (pH 7.0) at 25°C Agaricus bisporus
1.14.18.1 3.2
-
 L-Tyr-Gly-Gly in 0.1M sodium phosphate buffer (pH 7.0) at 25°C Trichoderma reesei
1.14.18.1 4
-
 L-Tyr-Gly-Gly in 0.1M sodium phosphate buffer (pH 7.0) at 25°C Agaricus bisporus
1.14.18.1 4.2
-
 Gly-L-Tyr-Gly in 0.1M sodium phosphate buffer (pH 7.0) at 25°C Trichoderma reesei
1.14.18.1 7.2
-
 Gly-Gly-L-Tyr in 0.1M sodium phosphate buffer (pH 7.0) at 25°C Trichoderma reesei

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.14.18.1 0.5
-
 L-Tyr-Gly-Gly in 0.1M sodium phosphate buffer (pH 7.0) at 25°C Trichoderma reesei
1.14.18.1 1.1
-
 glycyl-L-tyrosylglycine in 0.1M sodium phosphate buffer (pH 7.0) at 25°C Trichoderma reesei
1.14.18.1 2.4
-
 Gly-Gly-L-Tyr in 0.1M sodium phosphate buffer (pH 7.0) at 25°C Trichoderma reesei
1.14.18.1 17.1
-
 Gly-L-Tyr-Gly in 0.1 M sodium phosphate buffer (pH 7.0) at 25°C Agaricus bisporus
1.14.18.1 19
-
 L-Tyr-Gly-Gly in 0.1 M sodium phosphate buffer (pH 7.0) at 25°C Agaricus bisporus
1.14.18.1 26.5
-
 Gly-Gly-L-Tyr in 0.1 M sodium phosphate buffer (pH 7.0) at 25°C Agaricus bisporus