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Literature summary extracted from

  • Gosling, A.; Zachariou, M.; Straffon, M.
    Purification and characterisation of a 4-hydroxy benzaldehyde dehydrogenase cloned from Acinetobacter baylyi (2008), Enzyme Microb. Technol., 43, 417-422.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.1.7 K+ 125 mM KCl, 1.5 fold increase in activity Acinetobacter baylyi
1.2.1.7 Na+ 125 mM NaCl, 1.5 fold increase in activity Acinetobacter baylyi

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.7 into the vector pET-Duet1 for expression in Escherichia coli BL21DE3 cells Acinetobacter baylyi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.7 0.006
-
 4-hydroxybenzaldehyde apparent KM Acinetobacter baylyi
1.2.1.7 0.029
-
 benzaldehyde apparent KM Acinetobacter baylyi
1.2.1.7 0.037
-
 3,4-Dihydroxybenzaldehyde apparent KM Acinetobacter baylyi
1.2.1.7 0.044
-
 4-hydroxy-3-methoxybenzaldehyde apparent KM Acinetobacter baylyi
1.2.1.7 0.6
-
 NAD+ apparent KM Acinetobacter baylyi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.7 55000
-
 determined by SDS-PAGE Acinetobacter baylyi
1.2.1.7 116000
-
 determined by gel filtration Acinetobacter baylyi

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.7 Acinetobacter baylyi
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.7 on a His-Trap FF IMAC column Acinetobacter baylyi

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.7 3.2
-
 clarified lysate, total activity 37 U, protein concentration 5.77 mg/ml Acinetobacter baylyi
1.2.1.7 22
-
 IMAC eluted 4-hydroxy benzaldehyde dehydrogenase, total activity 35 Units, protein concentration 0.27 mg/ml Acinetobacter baylyi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.7 2,4-dihydroxybenzaldehyde + NAD+ + H2O
-
 Acinetobacter baylyi 2,4-dihydroxybenzoate + NADH + H+
-
 ?
1.2.1.7 3,4-dihydroxybenzaldehyde + NAD+ + H2O
-
 Acinetobacter baylyi 3,4-dihydroxybenzoate + NADH + H+
-
 ?
1.2.1.7 3-hydroxy-4-methoxybenzaldehyde + NAD+ + H2O
-
 Acinetobacter baylyi 3-hydroxy-4-methoxybenzoate + NADH + H+
-
 ?
1.2.1.7 3-hydroxybenzaldehyde + NAD+ + H2O
-
 Acinetobacter baylyi 3-hydroxybenzoate + NADH + H+
-
 ?
1.2.1.7 3-methoxybenzaldehyde + NAD+ + H2O
-
 Acinetobacter baylyi 3-methoxybenzoate + NADH + H+
-
 ?
1.2.1.7 4-hydroxy-3-methoxybenzaldehyde + NAD+ + H2O
-
 Acinetobacter baylyi 4-hydroxy-3-methoxybenzoate + NADH + H+
-
 ?
1.2.1.7 4-hydroxybenzaldehyde + NAD+ + H2O
-
 Acinetobacter baylyi 4-hydroxybenzoate + NADH + H+
-
 ?
1.2.1.7 benzaldehyde + NAD+ + H2O
-
 Acinetobacter baylyi benzoate + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.7 homodimer 2 * 55000 Acinetobacter baylyi

Synonyms

EC Number Synonyms Comment Organism
1.2.1.7 4-hydroxy benzaldehyde dehydrogenase
-
 Acinetobacter baylyi

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.7 21
-
 activity assay Acinetobacter baylyi

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.2.1.7 21 45 21°C 100%, 37°C 90%, 45°C 75% activity Acinetobacter baylyi

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2.1.7 4
-
 4-hydroxybenzaldehyde apparent Kcat Acinetobacter baylyi
1.2.1.7 4.6
-
 benzaldehyde apparent Kcat Acinetobacter baylyi
1.2.1.7 14
-
 NAD+ apparent Kcat Acinetobacter baylyi
1.2.1.7 18
-
 4-hydroxy-3-methoxybenzaldehyde apparent Kcat Acinetobacter baylyi
1.2.1.7 25
-
 3,4-Dihydroxybenzaldehyde apparent Kcat Acinetobacter baylyi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.7 9.3
-
-
 Acinetobacter baylyi

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.2.1.7 6.9 10.7 the activities measured at pH 6.85 and 10.7 are approximately 30% of the activity measured at pH 9.3 Acinetobacter baylyi

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.7 NAD+
-
 Acinetobacter baylyi

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.2.1.7 Acinetobacter baylyi determined by isoelectric focusing electrophoresis
-
 5.6
1.2.1.7 Acinetobacter baylyi predicted from the amino acid sequence
-
 5.8