EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.1.72 | expression in Escherichia coli DH5 alpha | Thermobifida fusca |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.1.72 | diisopropyl fluorophosphate | - |
Thermobifida fusca | |
3.1.1.72 | Hg2+ | - |
Thermobifida fusca | |
3.1.1.72 | phenylmethanesulfonyl fluoride | - |
Thermobifida fusca | |
3.1.1.72 | Zn2+ | - |
Thermobifida fusca |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.72 | 0.00186 | - |
p-nitrophenyl acetate | 30°C, pH 8.0 | Thermobifida fusca |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.1.1.72 | extracellular | - |
Thermobifida fusca | - |
- |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.1.72 | 28000 | - |
gel filtration on Sepharose CL-6B | Thermobifida fusca |
3.1.1.72 | 30000 | - |
SDS-PAGE | Thermobifida fusca |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.1.72 | Thermobifida fusca | - |
- |
- |
3.1.1.72 | Thermobifida fusca NTU22 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.1.72 | centrifugation, ultrafiltration, gel-filtration chromatography, 4°C | Thermobifida fusca |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.1.1.72 | culture supernatant | - |
Thermobifida fusca | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.1.72 | 25.6 | - |
purified enzyme | Thermobifida fusca |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.72 | acetylxylan + H2O | - |
Thermobifida fusca | ? | - |
? | |
3.1.1.72 | acetylxylan + H2O | - |
Thermobifida fusca NTU22 | ? | - |
? | |
3.1.1.72 | p-nitrophenyl acetate + H2O | - |
Thermobifida fusca | p-nitrophenol + acetate | - |
? | |
3.1.1.72 | p-nitrophenyl acetate + H2O | - |
Thermobifida fusca NTU22 | p-nitrophenol + acetate | - |
? | |
3.1.1.72 | p-nitrophenyl butyrate + H2O | - |
Thermobifida fusca | p-nitrophenol + butyrate | - |
? | |
3.1.1.72 | p-nitrophenyl butyrate + H2O | - |
Thermobifida fusca NTU22 | p-nitrophenol + butyrate | - |
? | |
3.1.1.72 | p-nitrophenyl caprylate + H2O | - |
Thermobifida fusca | p-nitrophenol + caprylate | - |
? | |
3.1.1.72 | p-nitrophenyl caprylate + H2O | - |
Thermobifida fusca NTU22 | p-nitrophenol + caprylate | - |
? | |
3.1.1.72 | p-nitrophenyl palmitate + H2O | - |
Thermobifida fusca | p-nitrophenol + palmitate | - |
? | |
3.1.1.72 | p-nitrophenyl palmitate + H2O | - |
Thermobifida fusca NTU22 | p-nitrophenol + palmitate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.1.72 | monomer | 1 * 30000, SDS-PAGE | Thermobifida fusca |
3.1.1.72 | monomer | 1 * 28000, gel filtration on Sepharose CL-6B | Thermobifida fusca |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.1.72 | acetylxylan esterase | - |
Thermobifida fusca |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.72 | 80 | - |
- |
Thermobifida fusca |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.72 | additional information | - |
about 70% of the original activity still remains after 3 h at 70°C | Thermobifida fusca |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.72 | 47.6 | - |
p-nitrophenyl acetate | 30°C, pH 8.0 | Thermobifida fusca |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.72 | 8 | - |
- |
Thermobifida fusca |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.72 | 5 | 9 | - |
Thermobifida fusca |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.72 | 5 | 9 | 4°C, 24 h | Thermobifida fusca |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
3.1.1.72 | Thermobifida fusca | isoelectric focusing gel electrophoresis | - |
6.55 |