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Literature summary extracted from

  • Yang, C.; Liu, W.
    Purification and properties of an acetylxylan esterase from Thermobifida fusca (2008), Enzyme Microb. Technol., 42, 181-186.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.72 expression in Escherichia coli DH5 alpha Thermobifida fusca

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.72 diisopropyl fluorophosphate
-
Thermobifida fusca
3.1.1.72 Hg2+
-
Thermobifida fusca
3.1.1.72 phenylmethanesulfonyl fluoride
-
Thermobifida fusca
3.1.1.72 Zn2+
-
Thermobifida fusca

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.72 0.00186
-
p-nitrophenyl acetate 30°C, pH 8.0 Thermobifida fusca

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.72 extracellular
-
Thermobifida fusca
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.72 28000
-
gel filtration on Sepharose CL-6B Thermobifida fusca
3.1.1.72 30000
-
SDS-PAGE Thermobifida fusca

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.72 Thermobifida fusca
-
-
-
3.1.1.72 Thermobifida fusca NTU22
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.72 centrifugation, ultrafiltration, gel-filtration chromatography, 4°C Thermobifida fusca

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.72 culture supernatant
-
Thermobifida fusca
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.72 25.6
-
purified enzyme Thermobifida fusca

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.72 acetylxylan + H2O
-
Thermobifida fusca ?
-
?
3.1.1.72 acetylxylan + H2O
-
Thermobifida fusca NTU22 ?
-
?
3.1.1.72 p-nitrophenyl acetate + H2O
-
Thermobifida fusca p-nitrophenol + acetate
-
?
3.1.1.72 p-nitrophenyl acetate + H2O
-
Thermobifida fusca NTU22 p-nitrophenol + acetate
-
?
3.1.1.72 p-nitrophenyl butyrate + H2O
-
Thermobifida fusca p-nitrophenol + butyrate
-
?
3.1.1.72 p-nitrophenyl butyrate + H2O
-
Thermobifida fusca NTU22 p-nitrophenol + butyrate
-
?
3.1.1.72 p-nitrophenyl caprylate + H2O
-
Thermobifida fusca p-nitrophenol + caprylate
-
?
3.1.1.72 p-nitrophenyl caprylate + H2O
-
Thermobifida fusca NTU22 p-nitrophenol + caprylate
-
?
3.1.1.72 p-nitrophenyl palmitate + H2O
-
Thermobifida fusca p-nitrophenol + palmitate
-
?
3.1.1.72 p-nitrophenyl palmitate + H2O
-
Thermobifida fusca NTU22 p-nitrophenol + palmitate
-
?

Subunits

EC Number Subunits Comment Organism
3.1.1.72 monomer 1 * 30000, SDS-PAGE Thermobifida fusca
3.1.1.72 monomer 1 * 28000, gel filtration on Sepharose CL-6B Thermobifida fusca

Synonyms

EC Number Synonyms Comment Organism
3.1.1.72 acetylxylan esterase
-
Thermobifida fusca

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.72 80
-
-
Thermobifida fusca

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.72 additional information
-
about 70% of the original activity still remains after 3 h at 70°C Thermobifida fusca

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.72 47.6
-
p-nitrophenyl acetate 30°C, pH 8.0 Thermobifida fusca

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.72 8
-
-
Thermobifida fusca

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.72 5 9
-
Thermobifida fusca

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.72 5 9 4°C, 24 h Thermobifida fusca

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.1.72 Thermobifida fusca isoelectric focusing gel electrophoresis
-
6.55