Literature summary extracted from

Kawamoto, H.; Oguma, T.; Sekine, H.; Kobayashi, M.
Immobilization of cycloisomaltooligosaccharide glucanotransferase for the production of cycloisomaltooligosaccharides from dextran (2001), Enzyme Microb. Technol., 28, 515-521.

Activating Compound

EC Number	Activating Compound	Comment	Organism	Structure
2.4.1.248	Ca2+	a Ca2+ concentration of 50-100 mM is optimal for the thermostability of the immobilized CITase, 10-50 mM for the free enzyme	Niallia circulans

Application

EC Number	Application	Comment	Organism
2.4.1.248	synthesis	immobilization of enzyme for production of cycloisomaltooligosaccharides. Immobilization on Chitopearl BCW-3505 results in 35% remaining activity, on Chitopearl BCW-3005 35%, Chitopearl SH-3505 28%, and Diaion HP-20 on 22% remaining activity. The maximum cycloisomaltooligosaccharides yield in batch reactions at 0.2, 2 and 10% dextran was 28, 24 and 12%, respectively. The concentration of linear oligosaccharides, the byproducts in the reaction mixture, isgreater with the immobilized CITase than the free enzyme. The immobilized CITase is less thermostable than the free enzyme by about 10°C	Niallia circulans

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.4.1.248	Niallia circulans	-	-	-

Synonyms

EC Number	Synonyms	Comment	Organism
2.4.1.248	CITase	-	Niallia circulans

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Release 2023.1 (January 2023)