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Literature summary extracted from

  • Harris, T.R.; Aronov, P.A.; Hammock, B.D.
    Soluble epoxide hydrolase homologs in Strongylocentrotus purpuratus suggest a gene duplication event and subsequent divergence (2008), DNA Cell Biol., 27, 467-477.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.3.2.10 cDNA extraction, RACE PCR with RNA-fragment of soluble epoxide hydrolase-like protein 1 (SPEH1), insertion into baculovirus transfection vector pACUW21 or pBacPAK8, recombinant baculoviruses generated by cotransfection of Spodoptera frugiperda Sf21 cells with described vector and Bsu361-cleaved BacPAK6 viral DNA, infection of Trichoplusia ni High Five cells Strongylocentrotus purpuratus
3.3.2.10 cDNA extraction, RACE PCR with RNA-fragment of soluble epoxide hydrolase-like protein 2 (SPEH2), insertion into baculovirus transfection vector pACUW21 or pBacPAK8, recombinant baculoviruses generated by cotransfection of Spodoptera frugiperda Sf21 cells with described vector and Bsu361-cleaved BacPAK6 viral DNA, infection of Trichoplusia ni High Five cells Strongylocentrotus purpuratus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.3.2.10 12-(3-adamantane-1-yl-ureido)-dodecanoic acid
-
 Strongylocentrotus purpuratus
3.3.2.10 N,N'-dicyclohexylurea
-
 Strongylocentrotus purpuratus
3.3.2.10 N-adamantyl-N'-cyclohexylurea
-
 Strongylocentrotus purpuratus
3.3.2.10 N-cyclohexyl(dodecylamino)carboxamide
-
 Strongylocentrotus purpuratus
3.3.2.10 N-cyclohexyl-N'-ethylurea
-
 Strongylocentrotus purpuratus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.3.2.10 additional information
-
 additional information SPEH1 displays no activity on substrate 2'-(2-benzothiazolyl)-6'-hydroxybenzothiazole phosphate, substrate used to assay human sEH N-terminal phosphatise activity Strongylocentrotus purpuratus
3.3.2.10 0.035
-
 trans-diphenyl-propene oxide
-
 Strongylocentrotus purpuratus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.3.2.10 63800
-
 predicted: 63600, SPEH1 Strongylocentrotus purpuratus
3.3.2.10 64200
-
 predicted: 64400, SPEH2 Strongylocentrotus purpuratus

Organism

EC Number Organism UniProt Comment Textmining
3.3.2.10 Strongylocentrotus purpuratus B2MWN2
-
-
3.3.2.10 Strongylocentrotus purpuratus B2MWN3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.3.2.10 Trichoplusia ni High Five cells homogenization by Ultra Turrax, talon metal affinity resin, concentration in a 30000 Da cut Centricon centrifugal filter unit, desalting with desalting column Strongylocentrotus purpuratus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.3.2.10 gonad
-
 Strongylocentrotus purpuratus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.3.2.10 0.033
-
 (5Z,11Z,14Z)-8,9-epoxyeicosa-8,11,14-trienoic acid Strongylocentrotus purpuratus
3.3.2.10 0.33
-
 (5Z,8Z,11Z)-14,15-epoxyeicosa-5,8,11-trienoic acid Strongylocentrotus purpuratus
3.3.2.10 0.49
-
 (5Z,8Z,13Z)-11,12-epoxyeicosa-5,8,14-trienoic acid Strongylocentrotus purpuratus
3.3.2.10 3.1
-
 trans-1,3-diphenylpropene oxide Strongylocentrotus purpuratus
3.3.2.10 4.2
-
 (9Z)-12,13-epoxyoctadec-9-enoic acid Strongylocentrotus purpuratus
3.3.2.10 4.8
-
 (12Z)-9,10-epoxyoctadec-12-enoic acid Strongylocentrotus purpuratus

Storage Stability

EC Number Storage Stability Organism
3.3.2.10 SPEH1 EH activity displays a half-life of 2 h at 37°C, of over 1 week at 4°C and of 1-2 days at room temperature Strongylocentrotus purpuratus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.3.2.10 (12Z)-9,10-epoxyoctadec-12-enoic acid
-
 Strongylocentrotus purpuratus (12Z)-9,10-dihydroxyoctadec-12-enoic acid
-
 ?
3.3.2.10 (5Z,11Z,14Z)-8,9-epoxyeicosa-8,11,14-trienoic acid + H2O
-
 Strongylocentrotus purpuratus (5Z,11Z,14Z)-8,9-dihydroxyeicosa-5,11,14-trienoic acid
-
 ?
3.3.2.10 (5Z,8Z,11Z)-14,15-epoxyeicosa-5,8,11-trienoic acid + H2O
-
 Strongylocentrotus purpuratus (5Z,8Z,11Z)-14,15-dihydroxyeicosa-5,8,11-trienoic acid
-
 ?
3.3.2.10 (5Z,8Z,14Z)-11,12-epoxyeicosa-5,8,14-trienoic acid + H2O
-
 Strongylocentrotus purpuratus (5Z,8Z,14Z)-11,12-dihydroxyeicosa-5,8,14-trienoic acid
-
 ?
3.3.2.10 (9Z)-12,13-epoxyoctadec-9-enoic acid + H2O
-
 Strongylocentrotus purpuratus (9Z)-12,13-dihydroxyoctadec-9-enoic acid
-
 ?
3.3.2.10 trans-1,3-diphenylpropene oxide + H2O tritium labeled for radiometric assay Strongylocentrotus purpuratus 1,3-diphenylpropane-1,2-diol
-
 ?
3.3.2.10 trans-diphenyl-propene oxide + H2O
-
 Strongylocentrotus purpuratus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.3.2.10 SPEH1 epoxide hydrolase Strongylocentrotus purpuratus
3.3.2.10 SPEH2 epoxide hydrolase Strongylocentrotus purpuratus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.3.2.10 additional information
-
 SPEH1 EH activity displays a half-life of 1-2 days at room temperature Strongylocentrotus purpuratus
3.3.2.10 4
-
 SPEH1 EH activity displays a half-life of over one week Strongylocentrotus purpuratus
3.3.2.10 37
-
 SPEH1 EH activity displays a half-life of 2 h Strongylocentrotus purpuratus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.3.2.10 44
-
 trans-1,3-diphenylpropene oxide
-
 Strongylocentrotus purpuratus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.3.2.10 7.4
-
 for SPEH1 epxide hydrolase activity Strongylocentrotus purpuratus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.3.2.10 additional information
-
 additional information SPEH1 displays the same pattern of inhibition as the nematode soluble epoxide hydrolase homolog CEEH1 Strongylocentrotus purpuratus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.3.2.10 Strongylocentrotus purpuratus to 5.9 range, crude extracts from gonads, supernatant
-
 5.7
3.3.2.10 Strongylocentrotus purpuratus to 6.1 range, recombinant SPEH1, His6-tagged
-
 5.9

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.3.2.10 0.000057
-
 recombinant SPEH1 Strongylocentrotus purpuratus 12-(3-adamantane-1-yl-ureido)-dodecanoic acid
3.3.2.10 0.000059
-
 crude extract from gonads Strongylocentrotus purpuratus 12-(3-adamantane-1-yl-ureido)-dodecanoic acid
3.3.2.10 0.00069
-
 crude extract from gonads Strongylocentrotus purpuratus N-cyclohexyl(dodecylamino)carboxamide
3.3.2.10 0.00091
-
 recombinant SPEH1 Strongylocentrotus purpuratus N-cyclohexyl(dodecylamino)carboxamide
3.3.2.10 0.0023
-
 recombinant SPEH1 Strongylocentrotus purpuratus N-adamantyl-N'-cyclohexylurea
3.3.2.10 0.05
-
 crude extract from gonads Strongylocentrotus purpuratus N-cyclohexyl-N'-ethylurea
3.3.2.10 0.05
-
 more than the value, recombinant SPEH1 Strongylocentrotus purpuratus N,N'-dicyclohexylurea
3.3.2.10 0.05
-
 more than the value, recombinant SPEH1 Strongylocentrotus purpuratus N-cyclohexyl-N'-ethylurea