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Literature summary extracted from

  • Perez-Avalos, O.; Sanchez-Herrera, L.M.; Salgado, L.M.; Ponce-Noyola, T.
    A bifunctional endoglucanase/endoxylanase from Cellulomonas flavigena with potential use in industrial processes at different pH (2008), Curr. Microbiol., 57, 39-44.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.8 additional information Cellulomonas flavigena CDBB-531 secrets a bifunctional cellulase/xylanase Cellulomonas flavigena

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.8 Escherichia coli DH5alpha as transforming host, Escherichia coli XLI-Blue MRA (P2), cloning vector pBluescript SKII, 37°C, Luria-Bertoni broth Cellulomonas flavigena
3.2.1.136 Escherichia coli DH5alpha as transforming host, Escherichia coli XLI-Blue MRA (P2), cloning vector pBluescript SKII, 37 degrees Celsius, Luria-Bertoni broth Cellulomonas flavigena

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.8 phosphate buffer has a negative effect on the xylanase activity, as it reduces the activity around to 60% Cellulomonas flavigena

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.8 additional information
-
additional information KM for substrate Remazol brilliant blue-carboxymethylcellulose: 0.16 mg/ml, KM for substrate xylose: 0.72 mg/ml Cellulomonas flavigena
3.2.1.136 additional information
-
cellulose 0.16 mg/ml, Vmax = 971 micromol/min/mg protein Cellulomonas flavigena
3.2.1.136 additional information
-
xylose 0.72 mg/ml, Vmax = 2854 micromol/min/mg protein Cellulomonas flavigena

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.8 49000
-
SDS-PAGE Cellulomonas flavigena
3.2.1.136 49000
-
SDS-PAGE Cellulomonas flavigena

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.8 additional information Cellulomonas flavigena or beta-1,4-glycosidic linkages in major plant structural polysaccharides ?
-
?
3.2.1.8 additional information Cellulomonas flavigena CDBB-531 or beta-1,4-glycosidic linkages in major plant structural polysaccharides ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 Cellulomonas flavigena A1XM14
-
-
3.2.1.8 Cellulomonas flavigena A1XM14 fragment of 387 bp, part of the gene cxo; CDBB-531
-
3.2.1.8 Cellulomonas flavigena CDBB-531 A1XM14
-
-
3.2.1.8 Cellulomonas flavigena CDBB-531 A1XM14 fragment of 387 bp, part of the gene cxo; CDBB-531
-
3.2.1.136 Cellulomonas flavigena A1XM14 fragment of 387 bp, part of the gene cxo; CDBB-531
-
3.2.1.136 Cellulomonas flavigena CDBB-531 A1XM14 fragment of 387 bp, part of the gene cxo; CDBB-531
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.8 by gel filtration to apparent homogeneity, 39fold with 11% yield Cellulomonas flavigena
3.2.1.8 centrifuged supernatant concentrated with membrane filter, applied to Bio-Gel P100 gel filtration column, cellulolytic fractions pooled, lyophilized, dissolved, loaded onto hydroxylapatite column, last steps repeated, 4°C, purity monitored by SDS-PAGE and isoelectric focusing Cellulomonas flavigena
3.2.1.136 centrifuged supernatant concentrated with membrane filter, applied to Bio-Gel P100 gel filtration column, cellulolytic fractions pooled, lyophilized, dissolved, loaded onto hydroxylapatite column, last steps repeated, 4 degrees Celsius, purity monitored by SDS-PAGE and isoelectric focusing Cellulomonas flavigena

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.8 birchwood xylan + H2O
-
Cellulomonas flavigena D-xylose + L-arabinose + xylobiose + ?
-
?
3.2.1.8 birchwood xylan + H2O
-
Cellulomonas flavigena CDBB-531 D-xylose + L-arabinose + xylobiose + ?
-
?
3.2.1.8 additional information or beta-1,4-glycosidic linkages in major plant structural polysaccharides Cellulomonas flavigena ?
-
?
3.2.1.8 additional information or beta-1,4-glycosidic linkages in major plant structural polysaccharides Cellulomonas flavigena CDBB-531 ?
-
?
3.2.1.8 remazol brilliant blue xylan + H2O
-
Cellulomonas flavigena ?
-
?
3.2.1.8 remazol brilliant blue xylan + H2O
-
Cellulomonas flavigena CDBB-531 ?
-
?
3.2.1.8 Remazol brilliant blue-carboxymethylcellulose + H2O
-
Cellulomonas flavigena D-glucose + cellobiose + cellotriose + cellotetraose + high-molecular-mass oligosaccharides cellulose activity ?
3.2.1.8 Remazol brilliant blue-carboxymethylcellulose + H2O
-
Cellulomonas flavigena CDBB-531 D-glucose + cellobiose + cellotriose + cellotetraose + high-molecular-mass oligosaccharides cellulose activity ?
3.2.1.8 xylan + H2O xylan from birchwood Cellulomonas flavigena D-xylose + xylobiose + high-molecular-mass oligoxyloside xylanase activity ?
3.2.1.8 xylan + H2O xylan from birchwood Cellulomonas flavigena CDBB-531 D-xylose + xylobiose + high-molecular-mass oligoxyloside xylanase activity ?
3.2.1.136 Remazol brilliant blue-carboxymethylcellulose + H2O
-
Cellulomonas flavigena glucose + cellobiose + cellotriose + cellotetraose + high-molecular-mass oligosaccharides cellulose activity ?
3.2.1.136 xylan + H2O xylan from birchwood Cellulomonas flavigena xylose + arabinose + xylobiose + high-molecular-mass oligosaccharides xylanase activity ?

Subunits

EC Number Subunits Comment Organism
3.2.1.8 ? x * 49000, SDS-PAGE Cellulomonas flavigena
3.2.1.8 monomer 1 * 49000, SDS-PAGE Cellulomonas flavigena

Synonyms

EC Number Synonyms Comment Organism
3.2.1.8 beta-1,4-endoxylanase
-
Cellulomonas flavigena
3.2.1.8 bifunctional cellulase/xylanase
-
Cellulomonas flavigena
3.2.1.8 endoxylanase
-
Cellulomonas flavigena
3.2.1.8 xylanase
-
Cellulomonas flavigena
3.2.1.136 beta-1,4-endoxylanase
-
Cellulomonas flavigena
3.2.1.136 bifunctional cellulase/xylanase
-
Cellulomonas flavigena
3.2.1.136 endoglucanase
-
Cellulomonas flavigena
3.2.1.136 endoxylanase
-
Cellulomonas flavigena

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.8 50
-
-
Cellulomonas flavigena
3.2.1.8 50
-
xylanase activity Cellulomonas flavigena
3.2.1.136 50
-
for cellulase and xylanase activity Cellulomonas flavigena

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.8 30 70
-
Cellulomonas flavigena
3.2.1.136 30 70
-
Cellulomonas flavigena

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.8 7
-
highest xylanase activity in citrate-phosphate buffer, 10% less activity than in Tris-HCl Cellulomonas flavigena
3.2.1.8 9
-
highest activity at pH 9 in Tris-HCl buffer, whereas in citrate-phosphate buffer at pH 7 the best activity is 90% of that observed in Tris-HCl Cellulomonas flavigena
3.2.1.8 9
-
highest xylanase activity in Tris-HCl buffer Cellulomonas flavigena
3.2.1.136 6
-
highest cellulase activity in citrate-phosphate buffer, in Tris-HCl buffer only 50% activity Cellulomonas flavigena
3.2.1.136 7
-
highest cellulase activity in phosphate buffer, about 10% less activity than in citrate-phosphate buffer Cellulomonas flavigena
3.2.1.136 7
-
highest xylanase activity in citrate-phosphate buffer, 10% less activity than in Tris-HCl Cellulomonas flavigena
3.2.1.136 9
-
highest xylanase activity in Tris-HCl buffer Cellulomonas flavigena

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.8 5 10 pH 5-7: sodium citrate-phosphate buffer, pH 5-8: phosphate buffer, pH 7-10: Tris-HCl buffer Cellulomonas flavigena
3.2.1.136 5 10 pH 5-7: sodium citrate-phosphate buffer, pH 5-8: phosphate buffer, pH 7-10: Tris-HCl buffer Cellulomonas flavigena

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.8 Cellulomonas flavigena isoelectric focusing
-
4.3
3.2.1.8 Cellulomonas flavigena isoelectric focusing in 5% polyacrylamide gel, pH 3.5-10 Ampholine, 200 V, 50 min, bands visualized with Coomassie brilliant blue G250 or silver staining, standard proteins of pI 3.5-9.3
-
4.3
3.2.1.136 Cellulomonas flavigena isoelectric focusing in 5% polyacrylamide gel, pH 3.5-10 Ampholine, 200 V, 50 min, bands visualized with Coomassie brilliant blue G250 or silver staining, standard proteins of pI 3.5-9.3
-
4.3