EC Number | Application | Comment | Organism |
---|---|---|---|
3.2.1.8 | additional information | Cellulomonas flavigena CDBB-531 secrets a bifunctional cellulase/xylanase | Cellulomonas flavigena |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.8 | Escherichia coli DH5alpha as transforming host, Escherichia coli XLI-Blue MRA (P2), cloning vector pBluescript SKII, 37°C, Luria-Bertoni broth | Cellulomonas flavigena |
3.2.1.136 | Escherichia coli DH5alpha as transforming host, Escherichia coli XLI-Blue MRA (P2), cloning vector pBluescript SKII, 37 degrees Celsius, Luria-Bertoni broth | Cellulomonas flavigena |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.8 | phosphate buffer | has a negative effect on the xylanase activity, as it reduces the activity around to 60% | Cellulomonas flavigena |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.8 | additional information | - |
additional information | KM for substrate Remazol brilliant blue-carboxymethylcellulose: 0.16 mg/ml, KM for substrate xylose: 0.72 mg/ml | Cellulomonas flavigena | |
3.2.1.136 | additional information | - |
cellulose | 0.16 mg/ml, Vmax = 971 micromol/min/mg protein | Cellulomonas flavigena | |
3.2.1.136 | additional information | - |
xylose | 0.72 mg/ml, Vmax = 2854 micromol/min/mg protein | Cellulomonas flavigena |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.8 | 49000 | - |
SDS-PAGE | Cellulomonas flavigena |
3.2.1.136 | 49000 | - |
SDS-PAGE | Cellulomonas flavigena |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.8 | additional information | Cellulomonas flavigena | or beta-1,4-glycosidic linkages in major plant structural polysaccharides | ? | - |
? | |
3.2.1.8 | additional information | Cellulomonas flavigena CDBB-531 | or beta-1,4-glycosidic linkages in major plant structural polysaccharides | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.8 | Cellulomonas flavigena | A1XM14 | - |
- |
3.2.1.8 | Cellulomonas flavigena | A1XM14 | fragment of 387 bp, part of the gene cxo; CDBB-531 | - |
3.2.1.8 | Cellulomonas flavigena CDBB-531 | A1XM14 | - |
- |
3.2.1.8 | Cellulomonas flavigena CDBB-531 | A1XM14 | fragment of 387 bp, part of the gene cxo; CDBB-531 | - |
3.2.1.136 | Cellulomonas flavigena | A1XM14 | fragment of 387 bp, part of the gene cxo; CDBB-531 | - |
3.2.1.136 | Cellulomonas flavigena CDBB-531 | A1XM14 | fragment of 387 bp, part of the gene cxo; CDBB-531 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.8 | by gel filtration to apparent homogeneity, 39fold with 11% yield | Cellulomonas flavigena |
3.2.1.8 | centrifuged supernatant concentrated with membrane filter, applied to Bio-Gel P100 gel filtration column, cellulolytic fractions pooled, lyophilized, dissolved, loaded onto hydroxylapatite column, last steps repeated, 4°C, purity monitored by SDS-PAGE and isoelectric focusing | Cellulomonas flavigena |
3.2.1.136 | centrifuged supernatant concentrated with membrane filter, applied to Bio-Gel P100 gel filtration column, cellulolytic fractions pooled, lyophilized, dissolved, loaded onto hydroxylapatite column, last steps repeated, 4 degrees Celsius, purity monitored by SDS-PAGE and isoelectric focusing | Cellulomonas flavigena |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.8 | birchwood xylan + H2O | - |
Cellulomonas flavigena | D-xylose + L-arabinose + xylobiose + ? | - |
? | |
3.2.1.8 | birchwood xylan + H2O | - |
Cellulomonas flavigena CDBB-531 | D-xylose + L-arabinose + xylobiose + ? | - |
? | |
3.2.1.8 | additional information | or beta-1,4-glycosidic linkages in major plant structural polysaccharides | Cellulomonas flavigena | ? | - |
? | |
3.2.1.8 | additional information | or beta-1,4-glycosidic linkages in major plant structural polysaccharides | Cellulomonas flavigena CDBB-531 | ? | - |
? | |
3.2.1.8 | remazol brilliant blue xylan + H2O | - |
Cellulomonas flavigena | ? | - |
? | |
3.2.1.8 | remazol brilliant blue xylan + H2O | - |
Cellulomonas flavigena CDBB-531 | ? | - |
? | |
3.2.1.8 | Remazol brilliant blue-carboxymethylcellulose + H2O | - |
Cellulomonas flavigena | D-glucose + cellobiose + cellotriose + cellotetraose + high-molecular-mass oligosaccharides | cellulose activity | ? | |
3.2.1.8 | Remazol brilliant blue-carboxymethylcellulose + H2O | - |
Cellulomonas flavigena CDBB-531 | D-glucose + cellobiose + cellotriose + cellotetraose + high-molecular-mass oligosaccharides | cellulose activity | ? | |
3.2.1.8 | xylan + H2O | xylan from birchwood | Cellulomonas flavigena | D-xylose + xylobiose + high-molecular-mass oligoxyloside | xylanase activity | ? | |
3.2.1.8 | xylan + H2O | xylan from birchwood | Cellulomonas flavigena CDBB-531 | D-xylose + xylobiose + high-molecular-mass oligoxyloside | xylanase activity | ? | |
3.2.1.136 | Remazol brilliant blue-carboxymethylcellulose + H2O | - |
Cellulomonas flavigena | glucose + cellobiose + cellotriose + cellotetraose + high-molecular-mass oligosaccharides | cellulose activity | ? | |
3.2.1.136 | xylan + H2O | xylan from birchwood | Cellulomonas flavigena | xylose + arabinose + xylobiose + high-molecular-mass oligosaccharides | xylanase activity | ? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.8 | ? | x * 49000, SDS-PAGE | Cellulomonas flavigena |
3.2.1.8 | monomer | 1 * 49000, SDS-PAGE | Cellulomonas flavigena |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.8 | beta-1,4-endoxylanase | - |
Cellulomonas flavigena |
3.2.1.8 | bifunctional cellulase/xylanase | - |
Cellulomonas flavigena |
3.2.1.8 | endoxylanase | - |
Cellulomonas flavigena |
3.2.1.8 | xylanase | - |
Cellulomonas flavigena |
3.2.1.136 | beta-1,4-endoxylanase | - |
Cellulomonas flavigena |
3.2.1.136 | bifunctional cellulase/xylanase | - |
Cellulomonas flavigena |
3.2.1.136 | endoglucanase | - |
Cellulomonas flavigena |
3.2.1.136 | endoxylanase | - |
Cellulomonas flavigena |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.8 | 50 | - |
- |
Cellulomonas flavigena |
3.2.1.8 | 50 | - |
xylanase activity | Cellulomonas flavigena |
3.2.1.136 | 50 | - |
for cellulase and xylanase activity | Cellulomonas flavigena |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.8 | 30 | 70 | - |
Cellulomonas flavigena |
3.2.1.136 | 30 | 70 | - |
Cellulomonas flavigena |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.8 | 7 | - |
highest xylanase activity in citrate-phosphate buffer, 10% less activity than in Tris-HCl | Cellulomonas flavigena |
3.2.1.8 | 9 | - |
highest activity at pH 9 in Tris-HCl buffer, whereas in citrate-phosphate buffer at pH 7 the best activity is 90% of that observed in Tris-HCl | Cellulomonas flavigena |
3.2.1.8 | 9 | - |
highest xylanase activity in Tris-HCl buffer | Cellulomonas flavigena |
3.2.1.136 | 6 | - |
highest cellulase activity in citrate-phosphate buffer, in Tris-HCl buffer only 50% activity | Cellulomonas flavigena |
3.2.1.136 | 7 | - |
highest cellulase activity in phosphate buffer, about 10% less activity than in citrate-phosphate buffer | Cellulomonas flavigena |
3.2.1.136 | 7 | - |
highest xylanase activity in citrate-phosphate buffer, 10% less activity than in Tris-HCl | Cellulomonas flavigena |
3.2.1.136 | 9 | - |
highest xylanase activity in Tris-HCl buffer | Cellulomonas flavigena |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.8 | 5 | 10 | pH 5-7: sodium citrate-phosphate buffer, pH 5-8: phosphate buffer, pH 7-10: Tris-HCl buffer | Cellulomonas flavigena |
3.2.1.136 | 5 | 10 | pH 5-7: sodium citrate-phosphate buffer, pH 5-8: phosphate buffer, pH 7-10: Tris-HCl buffer | Cellulomonas flavigena |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
3.2.1.8 | Cellulomonas flavigena | isoelectric focusing | - |
4.3 |
3.2.1.8 | Cellulomonas flavigena | isoelectric focusing in 5% polyacrylamide gel, pH 3.5-10 Ampholine, 200 V, 50 min, bands visualized with Coomassie brilliant blue G250 or silver staining, standard proteins of pI 3.5-9.3 | - |
4.3 |
3.2.1.136 | Cellulomonas flavigena | isoelectric focusing in 5% polyacrylamide gel, pH 3.5-10 Ampholine, 200 V, 50 min, bands visualized with Coomassie brilliant blue G250 or silver staining, standard proteins of pI 3.5-9.3 | - |
4.3 |