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Literature summary extracted from

  • Hu, Z.; Lee, K.S.; Choo, Y.M.; Yoon, H.J.; Kim, I.; Wei, Y.D.; Gui, Z.Z.; Zhang, G.Z.; Sohn, H.D.; Jin, B.R.
    Molecular characterization of a phospholipid-hydroperoxide glutathione peroxidase from the bumblebee Bombus ignitus (2010), Comp. Biochem. Physiol. B, 155, 54-61.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.12 the baculovirus expression vector system, using the Autographa californica nucleopolyhedrovirus and the Sf9 insect cell line, is used to produce recombinant Bi-PHGPx proteins, the cDNA fragment containing the full-length open reading frame is inserted into the transfer vector pBAC1 Bombus ignitus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.12 0.0002353
-
 phosphatidylcholine hydroperoxide apparent KM Bombus ignitus
1.11.1.12 0.0008656
-
 H2O2 apparent KM Bombus ignitus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.11.1.12 selenium
-
 Bombus ignitus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.12 19000
-
 determined by SDS-PAGE Bombus ignitus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.11.1.12 glutathione + a lipid hydroperoxide Bombus ignitus
-
 glutathione disulfide + lipid + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.12 Bombus ignitus C3VVL8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.12 using a HisTrap column Bombus ignitus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.11.1.12 epidermis
-
 Bombus ignitus
-
1.11.1.12 fat body extraction of genomic DNA Bombus ignitus
-
1.11.1.12 hemolymph
-
 Bombus ignitus
-
1.11.1.12 midgut
-
 Bombus ignitus
-
1.11.1.12 muscle
-
 Bombus ignitus
-
1.11.1.12 whole body cDNA library Bombus ignitus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.12 glutathione + a lipid hydroperoxide
-
 Bombus ignitus glutathione disulfide + lipid + H2O
-
 ?
1.11.1.12 glutathione + H2O2 activity assay Bombus ignitus ?
-
 ?
1.11.1.12 glutathione + phosphatidylcholine hydroperoxide activity assay Bombus ignitus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.11.1.12 Bi-PHGPx
-
 Bombus ignitus
1.11.1.12 GPx4
-
 Bombus ignitus
1.11.1.12 PHGPX
-
 Bombus ignitus
1.11.1.12 phospholipid-hydroperoxide glutathione peroxidase
-
 Bombus ignitus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.11.1.12 30
-
-
 Bombus ignitus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.11.1.12 40
-
 thermal stability shows that the activity is significantly decreased when the temperature is higher than 40°C Bombus ignitus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.12 6.5
-
-
 Bombus ignitus
1.11.1.12 7.5
-
 activity assay Bombus ignitus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.11.1.12 4 11
-
 Bombus ignitus

Cofactor

EC Number Cofactor Comment Organism Structure
1.11.1.12 glutathione
-
 Bombus ignitus

Expression

EC Number Organism Comment Expression
1.11.1.12 Bombus ignitus Bi-PHGPx transcripts are up-regulated by stresses, such as wounding, H2O2 exposure, external temperature shock, and starvation up