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Literature summary extracted from

  • Song, J.M.; Nam, K.W.; Kang, S.G.; Kim, C.G.; Kwon, S.T.; Lee, Y.H.
    Molecular cloning and characterization of a novel cold-active beta-1,4-D-mannanase from the Antarctic springtail, Cryptopygus antarcticus (2008), Comp. Biochem. Physiol. B, 151, 32-40.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.78 exprression in Escherichia coli fused with thioredoxin gene Cryptopygus antarcticus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.78 Ag+ 5 mM, 24.8% residual activity Cryptopygus antarcticus
3.2.1.78 Hg2+ 5 mM, 16.4% residual activity Cryptopygus antarcticus
3.2.1.78 Mn2+ 5 mM, 32% residual activity Cryptopygus antarcticus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.78 Ca2+ 5 mM, 228% of initial activity Cryptopygus antarcticus
3.2.1.78 Cu2+ 5 mM, 151% of initial activity Cryptopygus antarcticus
3.2.1.78 Mg2+ 5 mM, 178% of initial activity Cryptopygus antarcticus
3.2.1.78 Zn2+ 5 mM, 220% of initial activity Cryptopygus antarcticus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.78 Cryptopygus antarcticus B4XC07
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.78 416.3
-
 30°C, pH 3.5 Cryptopygus antarcticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.78 locust bean gum + H2O
-
 Cryptopygus antarcticus ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.78 30
-
-
 Cryptopygus antarcticus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.78
-
 5 20-40% of maximum activity Cryptopygus antarcticus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.78 45
-
 half-life less than 10 min Cryptopygus antarcticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.78 3.5
-
-
 Cryptopygus antarcticus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.78 2.5 6 more than 50% of maximum activity Cryptopygus antarcticus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.78 7
-
 inactivation above Cryptopygus antarcticus