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Literature summary extracted from

  • Maeda, S.; Nakashima, A.; Yamada, R.; Hara, N.; Fujimoto, Y.; Ito, Y.; Sonobe, H.
    Molecular cloning of ecdysone 20-hydroxylase and expression pattern of the enzyme during embryonic development of silkworm Bombyx mori (2008), Comp. Biochem. Physiol. B, 149, 507-516.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.99.22 into a pSTBlue-1 Acceptor vector for sequencing, recombinant baculovirus harboring the BmE20OHase cDNA is constructed using the BAC-TO-BAC baculovirus expression system Bombyx mori

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.99.22 ecdysone + NADPH + O2 Bombyx mori
-
 20-hydroxyecdysone + NADP+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.99.22 Bombyx mori Q3LFR2
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.99.22 egg nondiapause egg Bombyx mori
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.99.22 ecdysone + NADPH + O2
-
 Bombyx mori 20-hydroxyecdysone + NADP+ + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.99.22 BmE20OHase
-
 Bombyx mori
1.14.99.22 E20OHase
-
 Bombyx mori
1.14.99.22 ecdysone 20-hydroxylase
-
 Bombyx mori
1.14.99.22 ecdysone 20-monooxygenase
-
 Bombyx mori

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.99.22 cytochrome P450
-
 Bombyx mori
1.14.99.22 NADPH
-
 Bombyx mori