EC Number | Cloned (Comment) | Organism |
---|---|---|
7.3.2.3 | inducible expression of Rv1739c in an Escherichia coli strain EWP44 lacking CysA does not increase sulfate uptake by intact cells. Expression of Rv1739c in a Mycobacterium bovis BCG strain lacking the ABC sulfate permease subunit CysA cannot complement sulfate auxotrophy. Induction of Rv1739c expression in Escherichia coli increases bacterial uptake of sulfate, but not Cl-, formate, or oxalate. Sulfate uptake is also increased by overexpression of the Rv1739c transmembrane domain, but not of the cytoplasmic C-terminal STAS domain | Mycobacterium tuberculosis |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
7.3.2.3 | additional information | mutation to serine of the three cysteine residues of Rv1739c does not affect magnitude, pH-dependence, or pharmacology of sulfate uptake | Mycobacterium tuberculosis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
7.3.2.3 | carbonyl cyanide 3-chloro-phenylhydrazone | - |
Mycobacterium tuberculosis | |
7.3.2.3 | additional information | no inhibition by bicarbonate, nitrate, or phosphate, or by the anion transport inhibitor NS3623 | Mycobacterium tuberculosis | |
7.3.2.3 | N-ethylmaleimide | - |
Mycobacterium tuberculosis | |
7.3.2.3 | selenate | - |
Mycobacterium tuberculosis | |
7.3.2.3 | sulfite | - |
Mycobacterium tuberculosis | |
7.3.2.3 | thiosulfate | - |
Mycobacterium tuberculosis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
7.3.2.3 | 0.004 | - |
sulfate | pH 6.0, recombinant enzyme | Mycobacterium tuberculosis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
7.3.2.3 | additional information | Na+-independent sulfate uptake | Mycobacterium tuberculosis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
7.3.2.3 | additional information | Mycobacterium tuberculosis | facilitation of bacterial sulfate uptake by Rv1739c requires CysA and its associated sulfate permease activity, thus Rv1739c may be a CysTWA-dependent sulfate transporter | ? | - |
? | |
7.3.2.3 | additional information | Mycobacterium tuberculosis H37Rv | facilitation of bacterial sulfate uptake by Rv1739c requires CysA and its associated sulfate permease activity, thus Rv1739c may be a CysTWA-dependent sulfate transporter | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
7.3.2.3 | Mycobacterium tuberculosis | - |
gene Rv1739c | - |
7.3.2.3 | Mycobacterium tuberculosis H37Rv | - |
gene Rv1739c | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
7.3.2.3 | additional information | - |
- |
Mycobacterium tuberculosis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
7.3.2.3 | ATP + H2O + sulfate/out | facilitation of bacterial sulfate uptake by Rv1739c requires CysA and its associated sulfate permease activity, thus Rv1739c may be a CysTWA-dependent sulfate transporter | Mycobacterium tuberculosis | ADP + phosphate + sulfate/in | - |
? | |
7.3.2.3 | ATP + H2O + sulfate/out | facilitation of bacterial sulfate uptake by Rv1739c requires CysA and its associated sulfate permease activity, thus Rv1739c may be a CysTWA-dependent sulfate transporter | Mycobacterium tuberculosis H37Rv | ADP + phosphate + sulfate/in | - |
? | |
7.3.2.3 | additional information | facilitation of bacterial sulfate uptake by Rv1739c requires CysA and its associated sulfate permease activity, thus Rv1739c may be a CysTWA-dependent sulfate transporter | Mycobacterium tuberculosis | ? | - |
? | |
7.3.2.3 | additional information | Na+-independent sulfate uptake | Mycobacterium tuberculosis | ? | - |
? | |
7.3.2.3 | additional information | facilitation of bacterial sulfate uptake by Rv1739c requires CysA and its associated sulfate permease activity, thus Rv1739c may be a CysTWA-dependent sulfate transporter | Mycobacterium tuberculosis H37Rv | ? | - |
? | |
7.3.2.3 | additional information | Na+-independent sulfate uptake | Mycobacterium tuberculosis H37Rv | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
7.3.2.3 | Rv1739c | - |
Mycobacterium tuberculosis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
7.3.2.3 | 6 | - |
- |
Mycobacterium tuberculosis |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
7.3.2.3 | 5 | 8 | no activity below pH 5.0 and above pH 8.0 | Mycobacterium tuberculosis |