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Literature summary extracted from

  • El-Hawari, Y.; Favia, A.D.; Pilka, E.S.; Kisiela, M.; Oppermann, U.; Martin, H.J.; Maser, E.
    Analysis of the substrate-binding site of human carbonyl reductases CBR1 and CBR3 by site-directed mutagenesis (2009), Chem. Biol. Interact., 178, 234-241.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.184 mutants overexpressed in Escherichia coli Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.184 additional information residues of CBR3 exchanged to the corresponding amino acids of CBR1. Mutation of nine residues (236-244) in the vicinity of the catalytic center and a proline (P230) in CBR3 to the corresponding residues of CBR1 reveals a much higher kcat/Km value (5.7 micromol/min) towards isatin Homo sapiens
1.1.1.184 P230W active towards isatin and 9,10-phenanthrenequinone as the wild-type Homo sapiens
1.1.1.184 V244M inactive towards menadione, active towards isatin and 9,10-phenanthrenequinone as the wild-type Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.184 0.0043
-
 9,10-phenanthrenequinone residues 142-143, 230, 236-244, 270 of CBR3 exchanged to the corresponding amino acids of CBR1 Homo sapiens
1.1.1.184 0.0078
-
 isatin wild-type CBR1 Homo sapiens
1.1.1.184 0.0089
-
 9,10-phenanthrenequinone residues 230, 236-244, 270 of CBR3 exchanged to the corresponding amino acids of CBR1 Homo sapiens
1.1.1.184 0.0092
-
 9,10-phenanthrenequinone residues 97-98, 230, 236-244 of CBR3 exchanged to the corresponding amino acids of CBR1 Homo sapiens
1.1.1.184 0.0225
-
 isatin residues 142-143, 230, 236-244, 270 of CBR3 exchanged to the corresponding amino acids of CBR1 Homo sapiens
1.1.1.184 0.0315
-
 isatin residues 142-143, 230, 236-244 of CBR3 exchanged to the corresponding amino acids of CBR1 Homo sapiens
1.1.1.184 0.0327
-
 isatin residues 230, 236-244, 270 of CBR3 exchanged to the corresponding amino acids of CBR1 Homo sapiens
1.1.1.184 0.0354
-
 9,10-phenanthrenequinone wild-type CBR1 Homo sapiens
1.1.1.184 0.0393
-
 9,10-phenanthrenequinone residues 142-143, 230, 236-244 of CBR3 exchanged to the corresponding amino acids of CBR1 Homo sapiens
1.1.1.184 0.0471
-
 isatin residues 230, 236-244 of CBR3 exchanged to the corresponding amino acids of CBR1 Homo sapiens
1.1.1.184 0.055
-
 isatin residues 97-98, 230, 236-244 of CBR3 exchanged to the corresponding amino acids of CBR1 Homo sapiens
1.1.1.184 0.0564
-
 9,10-phenanthrenequinone residues 230, 236–244 of CBR3 exchanged to the corresponding amino acids of CBR1 Homo sapiens
1.1.1.184 0.078
-
 9,10-phenanthrenequinone wild-type CBR3 and mutant V244M. Residues 236-244, 262-277, 236-244/262-277 or 230 of CBR3 exchanged to the corresponding amino acids of CBR1 Homo sapiens
1.1.1.184 1.6
-
 isatin residues 236-244 of CBR3 exchanged to the corresponding amino acids of CBR1 Homo sapiens
1.1.1.184 4
-
 isatin wild-type CBR3 and mutant V244M. Residues 262-277, 236-244/262-277 or 230 of CBR3 exchanged to the corresponding amino acids of CBR1 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.184 cytosol
-
 Homo sapiens 5829
-

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.184 Homo sapiens O75828
-
-
1.1.1.184 Homo sapiens P16152
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.184 mutants purfied by Ni-affinity chromatography Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.184 9,10-phenanthrenequinone + NADPH
-
 Homo sapiens ? + NADP+
-
 ?
1.1.1.184 isatin + NADPH
-
 Homo sapiens ? + NADP+
-
 ?
1.1.1.184 additional information inactive towards menadione Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.184 carbonyl reductase
-
 Homo sapiens
1.1.1.184 CBR1
-
 Homo sapiens
1.1.1.184 CBR3
-
 Homo sapiens