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Literature summary extracted from

  • Miura, T.; Itoh, Y.; Takada, M.; Tsutsui, H.; Yukimura, T.; Nishinaka, T.; Terada, T.
    Investigation of the role of the amino acid residue at position 230 for catalysis in monomeric carbonyl reductase 3 (2009), Chem. Biol. Interact., 178, 211-214.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.184 wild-type and mutant cloned into the pET-28a vector and expressed in Escherichia coli, BL21(DE3) pLysE cells Cricetulus griseus
1.1.1.184 wild-type and mutant cloned into the pET-28a vector and expressed in Escherichia coli, BL21(DE3) pLysE cells Homo sapiens
1.1.1.184 wild-type and mutant cloned into the pET-28a vector and expressed in Escherichia coli, BL21(DE3) pLysE cells Rattus norvegicus

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.184 P230W exhibits properties similar to the parent enzyme with regard to steroid specificities and kinetics toward substrates Homo sapiens
1.1.1.184 W230P exhibits properties similar to the parent enzyme with regard to steroid specificities and kinetics toward substrates Cricetulus griseus
1.1.1.184 W230P exhibits properties similar to the parent enzyme with regard to steroid specificities and kinetics toward substrates Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.184 0.000000053
-
 4-benzoylpyridine wild-type Homo sapiens
1.1.1.184 0.000000058
-
 4-benzoylpyridine mutant P230W Homo sapiens
1.1.1.184 0.000000067
-
 menadione wild-type Homo sapiens
1.1.1.184 0.000000079
-
 menadione mutant P230W Homo sapiens
1.1.1.184 0.000000089
-
 menadione wild-type Cricetulus griseus
1.1.1.184 0.000000097
-
 menadione mutant W230P Cricetulus griseus
1.1.1.184 0.0000003
-
 menadione wild-type and mutant W230P Rattus norvegicus
1.1.1.184 0.00000054
-
 4-benzoylpyridine mutant W230P Cricetulus griseus
1.1.1.184 0.00000062
-
 4-benzoylpyridine wild-type Cricetulus griseus
1.1.1.184 0.0000015
-
 4-benzoylpyridine wild-type Rattus norvegicus
1.1.1.184 0.0000018
-
 4-benzoylpyridine mutant W230P Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.184 Cricetulus griseus
-
-
-
1.1.1.184 Homo sapiens
-
-
-
1.1.1.184 Rattus norvegicus B2GV72 Sprague-Dawley rats
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.184 by Ni2+ chelating affinity column chromatography Cricetulus griseus
1.1.1.184 by Ni2+ chelating affinity column chromatography Homo sapiens
1.1.1.184 by Ni2+ chelating affinity column chromatography Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.184 liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.184 4-benzoylpyridine + NADPH + H+
-
 Cricetulus griseus (S)-alpha-phenyl-4-pyridylmethanol + NADP+
-
 ?
1.1.1.184 4-benzoylpyridine + NADPH + H+
-
 Homo sapiens (S)-alpha-phenyl-4-pyridylmethanol + NADP+
-
 ?
1.1.1.184 4-benzoylpyridine + NADPH + H+
-
 Rattus norvegicus (S)-alpha-phenyl-4-pyridylmethanol + NADP+
-
 ?
1.1.1.184 5alpha-androstane-17beta-ol-3-one + NADPH
-
 Cricetulus griseus ? + NADP+
-
 ?
1.1.1.184 5alpha-androstane-17beta-ol-3-one + NADPH
-
 Homo sapiens ? + NADP+
-
 ?
1.1.1.184 5alpha-androstane-17beta-ol-3-one + NADPH
-
 Rattus norvegicus ? + NADP+
-
 ?
1.1.1.184 5alpha-androstane-3,17-dione + NADPH
-
 Cricetulus griseus ? + NADP+
-
 ?
1.1.1.184 5alpha-androstane-3,17-dione + NADPH
-
 Homo sapiens ? + NADP+
-
 ?
1.1.1.184 5alpha-androstane-3,17-dione + NADPH
-
 Rattus norvegicus ? + NADP+
-
 ?
1.1.1.184 5beta-pregnan-3alpha-ol-20-one + NADPH
-
 Cricetulus griseus ? + NADP+
-
 ?
1.1.1.184 5beta-pregnan-3alpha-ol-20-one + NADPH
-
 Homo sapiens ? + NADP+
-
 ?
1.1.1.184 menadione + NADPH
-
 Cricetulus griseus ? + NADP+
-
 ?
1.1.1.184 menadione + NADPH
-
 Homo sapiens ? + NADP+
-
 ?
1.1.1.184 menadione + NADPH
-
 Rattus norvegicus ? + NADP+
-
 ?
1.1.1.184 additional information no activity with 5alpha-androstane-3alpha-ol-17-one Cricetulus griseus ?
-
 ?
1.1.1.184 additional information no activity with 5alpha-androstane-3alpha-ol-17-one Homo sapiens ?
-
 ?
1.1.1.184 additional information no activity with 5alpha-androstane-3alpha-ol-17-one Rattus norvegicus ?
-
 ?
1.1.1.184 additional information no activity with 5beta-pregnan-3alpha-ol-20-one Rattus norvegicus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.184 monomer
-
 Cricetulus griseus
1.1.1.184 monomer
-
 Homo sapiens
1.1.1.184 monomer
-
 Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
1.1.1.184 carbonyl reductase 3
-
 Cricetulus griseus
1.1.1.184 carbonyl reductase 3
-
 Homo sapiens
1.1.1.184 carbonyl reductase 3
-
 Rattus norvegicus
1.1.1.184 CBR3
-
 Cricetulus griseus
1.1.1.184 CBR3
-
 Homo sapiens
1.1.1.184 CBR3
-
 Rattus norvegicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.184 0.009167
-
 4-benzoylpyridine mutant P230W Homo sapiens
1.1.1.184 0.0103
-
 4-benzoylpyridine wild-type Homo sapiens
1.1.1.184 0.011
-
 menadione wild-type Homo sapiens
1.1.1.184 0.01217
-
 menadione mutant P230W Homo sapiens
1.1.1.184 0.04167
-
 menadione mutant W230P Cricetulus griseus
1.1.1.184 0.0483
-
 menadione wild-type Cricetulus griseus
1.1.1.184 0.063
-
 4-benzoylpyridine wild-type Cricetulus griseus
1.1.1.184 0.065
-
 4-benzoylpyridine mutant W230P Cricetulus griseus
1.1.1.184 0.183
-
 4-benzoylpyridine mutant W230P Rattus norvegicus
1.1.1.184 0.23
-
 4-benzoylpyridine wild-type Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.184 NADPH
-
 Cricetulus griseus
1.1.1.184 NADPH
-
 Homo sapiens
1.1.1.184 NADPH
-
 Rattus norvegicus