Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Miura, T.; Nishinaka, T.; Takama, M.; Murakami, M.; Terada, T.
    Chinese hamster monomeric carbonyl reductases of the short-chain dehydrogenase/reductase superfamily (2009), Chem. Biol. Interact., 178, 110-116.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.184 pET28a-CHCR-transformed Escherichia coli BL21(DE3)pLys cells Cricetulus griseus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.184 NADP+ NADPH-linked reductase activities of CHCRs are partly inhibited by 2 mM NADP+; NADPH-linked reductase activities of CHCRs are partly inhibited by 2 mM NADP+; NADPH-linked reductase activities of CHCRs are partly inhibited by 2 mM NADP+ Cricetulus griseus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.184 0.00183
-
 isatin at 100 mM sodium phosphate buffer, pH 6.5, 0.065 mM NADPH, 25°C Cricetulus griseus
1.1.1.184 0.00214
-
 NADPH at 100 mM sodium phosphate buffer, pH 6.5, 0.065 mM NADPH, 25°C Cricetulus griseus
1.1.1.184 0.0022
-
 NADPH at 100 mM sodium phosphate buffer, pH 6.5, 0.065 mM NADPH, 25°C Cricetulus griseus
1.1.1.184 0.0026
-
 isatin at 100 mM sodium phosphate buffer, pH 6.5, 0.065 mM NADPH, 25°C Cricetulus griseus
1.1.1.184 0.0218
-
 NADPH at 100 mM sodium phosphate buffer, pH 6.5, 0.065 mM NADPH, 25°C Cricetulus griseus
1.1.1.184 0.043
-
 4-benzoylpyridine at 100 mM sodium phosphate buffer, pH 6.5, 0.065 mM NADPH, 25°C Cricetulus griseus
1.1.1.184 0.078
-
 isatin at 100 mM sodium phosphate buffer, pH 6.5, 0.065 mM NADPH, 25°C Cricetulus griseus
1.1.1.184 0.12
-
 4-nitrobenzaldehyde at 100 mM sodium phosphate buffer, pH 6.5, 0.065 mM NADPH, 25°C Cricetulus griseus
1.1.1.184 0.3
-
 NADH at 100 mM sodium phosphate buffer, pH 6.5, 0.065 mM NADPH, 25°C Cricetulus griseus
1.1.1.184 0.338
-
 4-nitrobenzaldehyde at 100 mM sodium phosphate buffer, pH 6.5, 0.065 mM NADPH, 25°C Cricetulus griseus
1.1.1.184 0.354
-
 4-nitrobenzaldehyde at 100 mM sodium phosphate buffer, pH 6.5, 0.065 mM NADPH, 25°C Cricetulus griseus
1.1.1.184 0.386
-
 4-benzoylpyridine at 100 mM sodium phosphate buffer, pH 6.5, 0.065 mM NADPH, 25°C Cricetulus griseus
1.1.1.184 0.43
-
 NADH at 100 mM sodium phosphate buffer, pH 6.5, 0.065 mM NADPH, 25°C Cricetulus griseus
1.1.1.184 0.435
-
 4-benzoylpyridine at 100 mM sodium phosphate buffer, pH 6.5, 0.065 mM NADPH, 25°C Cricetulus griseus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.184 33950
-
 mass spectrometry, His-tagged CHCR2 Cricetulus griseus
1.1.1.184 34000
-
 mass spectrometry, His-tagged CHCR1 Cricetulus griseus
1.1.1.184 34040
-
 sequence analysis, His-tagged CHCR1 Cricetulus griseus
1.1.1.184 34080
-
 sequence analysis, His-tagged CHCR2 Cricetulus griseus
1.1.1.184 34300
-
 mass spectrometry, His-tagged CHCR3 Cricetulus griseus
1.1.1.184 34430
-
 sequence analysis, His-tagged CHCR3 Cricetulus griseus
1.1.1.184 35000
-
 1 * 35000, SDS-PAGE Cricetulus griseus
1.1.1.184 38000
-
 1 * 38000, SDS-PAGE Cricetulus griseus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.184 Cricetulus griseus Q924V2
-
-
1.1.1.184 Cricetulus griseus Q924V3
-
-
1.1.1.184 Cricetulus griseus Q9JJN7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.184 by metal-chelating affinity column chromatography and gel filtration Cricetulus griseus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.184 brain
-
 Cricetulus griseus
-
1.1.1.184 CHO-K1 cell
-
 Cricetulus griseus
-
1.1.1.184 kidney
-
 Cricetulus griseus
-
1.1.1.184 kidney high expression of CHCR1 at both the mRNA and protein levels Cricetulus griseus
-
1.1.1.184 kidney high expression of CHCR2 at both the mRNA and protein levels Cricetulus griseus
-
1.1.1.184 lung
-
 Cricetulus griseus
-
1.1.1.184 additional information CHCR1 is absent from uterus and ovary. CHCR1 shows a sex-dependent expression at the protein level Cricetulus griseus
-
1.1.1.184 ovary
-
 Cricetulus griseus
-
1.1.1.184 testis high expression of CHCR1 at both the mRNA and protein levels Cricetulus griseus
-
1.1.1.184 testis high expression of CHCR2 at both the mRNA and protein levels Cricetulus griseus
-
1.1.1.184 uterus
-
 Cricetulus griseus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.184 2-hydroxyacetophenone + NADH + H+
-
 Cricetulus griseus (S)-1-phenyl-1,2-ethanediol + NAD+
-
 ?
1.1.1.184 4-benzoylpyridine + NADPH + H+
-
 Cricetulus griseus (S)-phenyl(pyridin-4-yl)methanol + NADP+
-
 ?
1.1.1.184 4-nitrobenzaldehyde + NADPH
-
 Cricetulus griseus 4-nitrobenzyl alcohol + NADP+
-
 ?
1.1.1.184 isatin + NADPH
-
 Cricetulus griseus ? + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.184 monomer 1 * 34000, SDS-PAGE Cricetulus griseus
1.1.1.184 monomer 1 * 35000, SDS-PAGE Cricetulus griseus
1.1.1.184 monomer 1 * 38000, SDS-PAGE Cricetulus griseus

Synonyms

EC Number Synonyms Comment Organism
1.1.1.184 carbonyl reductase
-
 Cricetulus griseus
1.1.1.184 CHCR1
-
 Cricetulus griseus
1.1.1.184 CHCR2
-
 Cricetulus griseus
1.1.1.184 CHCR3
-
 Cricetulus griseus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.184 0.0283
-
 4-nitrobenzaldehyde at 100 mM sodium phosphate buffer, pH 6.5, 0.065 mM NADPH, 25°C Cricetulus griseus
1.1.1.184 0.053
-
 4-benzoylpyridine at 100 mM sodium phosphate buffer, pH 6.5, 0.065 mM NADPH, 25°C Cricetulus griseus
1.1.1.184 0.3
-
 4-nitrobenzaldehyde at 100 mM sodium phosphate buffer, pH 6.5, 0.065 mM NADPH, 25°C Cricetulus griseus
1.1.1.184 0.5167
-
 isatin at 100 mM sodium phosphate buffer, pH 6.5, 0.065 mM NADPH, 25°C Cricetulus griseus
1.1.1.184 0.583
-
 isatin at 100 mM sodium phosphate buffer, pH 6.5, 0.065 mM NADPH, 25°C Cricetulus griseus
1.1.1.184 1.03
-
 4-benzoylpyridine at 100 mM sodium phosphate buffer, pH 6.5, 0.065 mM NADPH, 25°C Cricetulus griseus
1.1.1.184 1.087
-
 4-nitrobenzaldehyde at 100 mM sodium phosphate buffer, pH 6.5, 0.065 mM NADPH, 25°C Cricetulus griseus
1.1.1.184 1.225
-
 isatin at 100 mM sodium phosphate buffer, pH 6.5, 0.065 mM NADPH, 25°C Cricetulus griseus
1.1.1.184 1.967
-
 4-benzoylpyridine at 100 mM sodium phosphate buffer, pH 6.5, 0.065 mM NADPH, 25°C Cricetulus griseus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.184 NAD(P)H
-
 Cricetulus griseus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.1.1.184 0.2
-
 at 2 mM Cricetulus griseus NADP+
1.1.1.184 2
-
 at 2 mM Cricetulus griseus NADP+