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Literature summary extracted from

  • Bubinas, A.; Giedraityte, G.; Kalediene, L.; Nivinskiene, O.; Butkiene, R.
    Degradation of naphthalene by thermophilic bacteria via a pathway, through protocatechuic acid (2008), Cent. Eur. J. Biol., 3, 61-68.
No PubMed abstract available

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.3 additional information Geobacillus sp. enzyme involved in last ring fission in naphtalene degradation, intermediates confirm proceeding through protocatechuic acid via ortho-claevage pathway ?
-
 ?
1.13.11.3 protocatechuic acid + O2 Geobacillus sp.
-
 3-carboxy-cis,cis-muconic acid
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.3 Geobacillus sp.
-
 thermophilic isolate G27 from high-temperature oilfield in Lithuania
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.3 cells harvested by centrifugation, washed twice with 50 mM sodium phosphate buffer, pH 7.0 containing 10% glycerol, disruption with solicitor, centrifuged, supernatant stored at -20°C Geobacillus sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.3 350
-
 activity at the 10th h of the exponential growth phase Geobacillus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.3 additional information enzyme involved in last ring fission in naphtalene degradation, intermediates confirm proceeding through protocatechuic acid via ortho-claevage pathway Geobacillus sp. ?
-
 ?
1.13.11.3 protocatechuic acid + O2
-
 Geobacillus sp. 3-carboxy-cis,cis-muconic acid
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.3 3,4-PCDase
-
 Geobacillus sp.
1.13.11.3 protocatchetuate 3,4-dioxygenase
-
 Geobacillus sp.