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Literature summary extracted from

  • Zhao, H.T.; Endo, S.; Ishikura, S.; Chung, R.; Hogg, P.J.; Hara, A.; El-Kabbani, O.
    Structure/function analysis of a critical disulfide bond in the active site of L-xylulose reductase (2009), Cell. Mol. Life Sci., 66, 1570-1579.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.10 expressed in Escherichia coli BL21 (DE3) cells Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.10 vapour diffusion method, using 15% PEG 8000, 0.05 M potassium phosphate and 0.1 M MES buffer, pH 6.5 Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.10 cysteine the addition of cysteine (more than 2 mM) inactivates human L-xylulose reductase and is accompanied by a 10fold decrease in catalytic efficiency, the activity of the cysteine-inactivated enzyme is not recovered by the addition of 10 mM dithiothreitol and 2-mercaptoethanol Homo sapiens
1.1.1.10 potassium phosphate when the purified enzyme containing 2 mM 2-mercaptoethanol is diluted with 10 mM potassium phosphate pH 7.0, its diacetyl reductase activity is gradually decreased Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.10 0.0945
-
 diacetyl wild type enzyme, in 10 mM potassium phosphate buffer Homo sapiens
1.1.1.10 0.22
-
 diacetyl mutant enzyme C138A, in 10 mM potassium phosphate buffer Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.10 Homo sapiens Q7Z4W1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.10
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.10 diacetyl + NADPH + H+
-
 Homo sapiens acetoin + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.10 dimer x-ray crystallography Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.10 NADPH
-
 Homo sapiens