EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.143 | additional information | inhibition of PARG in HeLa cells treated with 50 microM cell death inducing alkylating agent N-methyl-N'-nitro-N'-nitrosoguanidine leads (MNNG) increases poly(ADP-ribose) levels beyond control, untreated and MNNG-treated PARG-silenced cells show a tendency to larger amounts of long (more than 20 ADP-ribose units) polymers, and a slight increase in short and medium long polymers, however PARG-silencing has no effect on cell death, no effect on translocation of apoptosis-inducing factor (AIF) into nucleus | Homo sapiens | |
3.2.1.143 | siRNA | small interfering RNA, down regulation of PARG to 50% 24 h after siRNA transfection, maximum of 84% inhibition after 72 compared to negative control with ineffective scrambled siRNA, siRNA produced in vitro from cDNA with 21-nucleotide sequence target in human coding region of the enzyme | Homo sapiens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.143 | Homo sapiens | - |
human cervical carcinoma cells HeLa-S3 | - |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.2.1.143 | HeLa-S3 cell | - |
Homo sapiens | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.143 | poly(ADP-ribose) + H2O | P32-labelled poly(ADP-ribose) produced by PARP-1 (poly(ADP-ribose) polymerase) of labelled NAD+ | Homo sapiens | ADP-ribose + ADP-ribose oligomer | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.143 | PARG | - |
Homo sapiens |
3.2.1.143 | poly(ADP-ribose) glycohydrolase | - |
Homo sapiens |