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Literature summary extracted from

  • Levin, T.C.; Wickliffe, K.E.; Leppla, S.H.; Moayeri, M.
    Heat shock inhibits caspase-1 activity while also preventing its inflammasome-mediated activation by anthrax lethal toxin (2008), Cell. Microbiol., 10, 2434-2446.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.22.36 additional information nigericin-induces caspase-1 activation. Nalp1b or NLRP1b inflammasome-mediated activation of caspase-1 by anthrax lethal toxin, prevented by heat shock, overview Mus musculus

Application

EC Number Application Comment Organism
3.4.24.83 molecular biology the in vitro effects of thermal stress on the killing of murine macrophages by anthrax lethal toxin are investigated. Heat shock rapidly halts anthrax lethal toxin-induced cell death without any impact on toxin uptake or mitogen-activated protein kinases cleavage, by a mechanism independent of novel protein synthesis, p38 activation, HSP90 activity or proteasome inhibition. Rather, heat shock prevents the activation of procaspase-1 in anthrax lethal toxin -treated cells, apparently by the sequestration of pro-caspase-1 in a large, inhibitory complex. Heat-shocked cell lysates strongly inhibit the active caspase-1 heterotetramer in vitro, independent of a specific inflammasome platform. Results suggest the presence of a cellular, heat shock-inducible, caspase-1 inhibiting factor Bacillus anthracis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.36 additional information heat shock inhibits caspase-1 activity while also preventing its Nalp1b or NLRP1b inflammasome-mediated or nigericin-induced activation by anthrax lethal toxin, but once caspase-1 activation is initiated, heat shock is ineffective, time dependence, overview Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.22.36 pro-interleukin-1beta + H2O Mus musculus
-
 mature interleukin-1beta + ?
-
 ?
3.4.22.36 pro-interleukin-1beta + H2O Mus musculus BALB/cJ
-
 mature interleukin-1beta + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.36 Mus musculus
-
 BALB/cJ mice
-
3.4.22.36 Mus musculus BALB/cJ
-
 BALB/cJ mice
-
3.4.24.83 Bacillus anthracis
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.36 commercial preparation recombinant enzyme Mus musculus
-
3.4.22.36 macrophage bone marrow-derived macrophage, BMDM cell Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.36 pro-interleukin-1beta + H2O
-
 Mus musculus mature interleukin-1beta + ?
-
 ?
3.4.22.36 pro-interleukin-1beta + H2O caspase-1 effectively cleaves interleukin-1beta to its mature form in both heat shock and 37°C conditions Mus musculus mature interleukin-1beta + ?
-
 ?
3.4.22.36 pro-interleukin-1beta + H2O
-
 Mus musculus BALB/cJ mature interleukin-1beta + ?
-
 ?
3.4.22.36 pro-interleukin-1beta + H2O caspase-1 effectively cleaves interleukin-1beta to its mature form in both heat shock and 37°C conditions Mus musculus BALB/cJ mature interleukin-1beta + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.24.83 anthrax lethal toxin
-
 Bacillus anthracis