Literature summary extracted from
Liu, Y.L.; Jiang, S.; Ke, Z.M.; Wu, H.S.; Chi, C.W.; Guo, Z.Y.
Recombinant expression of a chitosanase and its application in chitosan oligosaccharide production (2009), Carbohydr. Res., 344, 815-819.
Application
EC Number |
Application |
Comment |
Organism |
---|
3.2.1.132 |
industry |
a cheap chitosanase for large-scale chitosan oligosaccharide production in industry |
Bacillus sp. (in: Bacteria) |
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
3.2.1.132 |
amplified DNA fragment ligated into a pGEM T-easy vector, subsequently subcloned from the T-vector into a pET vector, expressed as 6 x His tag fused at its N-terminal in the Escherichia coli strain BL21(DE3) as a soluble and active form |
Bacillus sp. (in: Bacteria) |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.2.1.132 |
Bacillus sp. (in: Bacteria) |
- |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
3.2.1.132 |
to homogeneity by immobilized metal-ion affinity chromatography |
Bacillus sp. (in: Bacteria) |
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
3.2.1.132 |
additional information |
- |
between 270-290 U/mg, recombinant chitosanase |
Bacillus sp. (in: Bacteria) |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
3.2.1.132 |
chitosan + H2O |
1 g of enzyme can hydrolyze about 100 kg of chitosan |
Bacillus sp. (in: Bacteria) |
chitosan oligosaccharides |
- |
? |
|
Temperature Stability [°C]
EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Comment |
Organism |
---|
3.2.1.132 |
40 |
50 |
its half-life is only several min at 60°C. At lower temperatures, the enzyme is quite stable, its half-life is extended to several hours at 50 and 40°C. No detectable activity loss at room temperature for several days |
Bacillus sp. (in: Bacteria) |