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Literature summary extracted from

  • Liu, Y.L.; Jiang, S.; Ke, Z.M.; Wu, H.S.; Chi, C.W.; Guo, Z.Y.
    Recombinant expression of a chitosanase and its application in chitosan oligosaccharide production (2009), Carbohydr. Res., 344, 815-819.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.132 industry a cheap chitosanase for large-scale chitosan oligosaccharide production in industry Bacillus sp. (in: Bacteria)

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.132 amplified DNA fragment ligated into a pGEM T-easy vector, subsequently subcloned from the T-vector into a pET vector, expressed as 6 x His tag fused at its N-terminal in the Escherichia coli strain BL21(DE3) as a soluble and active form Bacillus sp. (in: Bacteria)

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.132 Bacillus sp. (in: Bacteria)
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Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.132 to homogeneity by immobilized metal-ion affinity chromatography Bacillus sp. (in: Bacteria)

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.132 additional information
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between 270-290 U/mg, recombinant chitosanase Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.132 chitosan + H2O 1 g of enzyme can hydrolyze about 100 kg of chitosan Bacillus sp. (in: Bacteria) chitosan oligosaccharides
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Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.132 40 50 its half-life is only several min at 60°C. At lower temperatures, the enzyme is quite stable, its half-life is extended to several hours at 50 and 40°C. No detectable activity loss at room temperature for several days Bacillus sp. (in: Bacteria)