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Literature summary extracted from

  • Pesentseva, M.S.; Kusaykin, M.I.; Anastyuk, S.D.; Sova, V.V.; Zvyagintseva, T.N.
    Catalytic properties and mode of action of endo-(1-->3)-beta-D-glucanase and beta-D-glucosidase from the marine mollusk Littorina kurila (2008), Carbohydr. Res., 343, 2393-2400.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.21 200000
-
 x * 200000, SDS-PAGE Littorina kurila
3.2.1.39 32000
-
 gel filtration Littorina kurila
3.2.1.39 40000
-
 1 * 40000, SDS-PAGE Littorina kurila

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 Littorina kurila
-
 isoform G-II, exhibits both exo-glucanase and beta-D-glucosidase activities
-
3.2.1.39 Littorina kurila
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.39
-
 Littorina kurila

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.39 liver
-
 Littorina kurila
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.21 2.4
-
 pH 5.4 Littorina kurila
3.2.1.39 0.285
-
-
 Littorina kurila

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O 1000% of the activity with laminaran Littorina kurila 4-nitrophenol + beta-D-glucose
-
 ?
3.2.1.21 laminaran + H2O
-
 Littorina kurila D-glucose + ? single product, enzyme retains the anomeric center configuration ?
3.2.1.21 lichenan + H2O 80% of the activity with laminaran Littorina kurila ?
-
 ?
3.2.1.21 additional information isoform G-II, exhibits exo-glucanase and beta-D-glucosidase activities as well as transglycosylation activity Littorina kurila ?
-
 ?
3.2.1.21 translam + H2O 80% of the activity with laminaran Littorina kurila ?
-
 ?
3.2.1.39 laminaran + H2O
-
 Littorina kurila ?
-
 ?
3.2.1.39 additional information narrow substrate specificity, hydrolyzes only the (1-3)-beta-D-glucosidic bonds in the mixed (1-3),(1-6)- and (1-3),(1-4)-beta-D-glucans down to glucose and glucooligosaccharides. Enzyme acts with retention of the anomeric configuration and additionally catalyzes transglycosylation reactions Littorina kurila ?
-
 ?
3.2.1.39 periodate-oxidized laminaran + H2O 95% of the rate with laminaran Littorina kurila ?
-
 ?
3.2.1.39 translam + H2O 86% of the rate with laminaran Littorina kurila ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.21 ? x * 200000, SDS-PAGE Littorina kurila
3.2.1.39 monomer 1 * 40000, SDS-PAGE Littorina kurila

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.21 5.4
-
-
 Littorina kurila
3.2.1.39 5.4
-
-
 Littorina kurila

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.39 4.5 7.5
-
 Littorina kurila