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Literature summary extracted from

  • Zhang, Y.; Teoh, K.; Reed, D.; Covello, P.
    Molecular cloning and characterization of Dbr1, a 2-alkenal reductase from Artemisia annua (2009), Botany, 87, 643-649.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.74 expression in Escherichia coli Artemisia annua

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.74 0.0064
-
 2E-nonenal pH 7.0, 30°C Artemisia annua
1.3.1.74 0.071
-
 artemisinic aldehyde pH 7.0, 30°C Artemisia annua

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.74 38500
-
 x * 38500, calculated Artemisia annua

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.74 Artemisia annua
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.1.74 additional information Dbr1 expression is readily detectable in aerial parts of the plant, but not in the roots Artemisia annua
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.74 (2E)-4-hydroxynon-2-enal + NADPH + H+
-
 Artemisia annua 4-hydroxynonanal + NADP+
-
 ?
1.3.1.74 2E-hexenal + NADPH + H+ at 12% of the rate with 2E-nonenal Artemisia annua hexanal + NADP+
-
 ?
1.3.1.74 2E-nonenal + NADPH + H+
-
 Artemisia annua nonanal + NADP+
-
 ?
1.3.1.74 artemisinic aldehyde + NADPH + H+ at 1.9% of the rate with 2E-nonenal Artemisia annua dihydroartemisinic aldehyde + NADP+ 88% 11S- and 12% 11R-isomer ?

Subunits

EC Number Subunits Comment Organism
1.3.1.74 ? x * 38500, calculated Artemisia annua

Synonyms

EC Number Synonyms Comment Organism
1.3.1.74 Dbr1
-
 Artemisia annua
1.3.1.74 double bond reductase
-
 Artemisia annua

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.74 7
-
-
 Artemisia annua

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.74 additional information no cofactor: NADH Artemisia annua
1.3.1.74 NADPH
-
 Artemisia annua