BRENDA - Enzyme Database

Enzymatic, immunological and phylogenetic characterization of Brucella suis urease

Contreras-Rodriguez, A.; Quiroz-Limon, J.; Martins, A.M.; Peralta, H.; Avila-Calderon, E.; Sriranganathan, N.; Boyle, S.M.; Lopez-Merino, A.; BMC Microbiol. 8, 121 (2008)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.1.5
Acetohydroxamic acid
-
Brucella suis bv. 1
3.5.1.5
Hydroxyurea
-
Brucella suis bv. 1
3.5.1.5
Thiourea
-
Brucella suis bv. 1
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.1.5
5.6
-
Urea
by hyperbolic regression, Lineweaver-Burk: 5.24 mM
Brucella suis bv. 1
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.1.5
11129
-
x * 61054 + x * 11377 + x * 11129, calculated from sequence
Brucella suis bv. 1
3.5.1.5
11377
-
x * 61054 + x * 11377 + x * 11129, calculated from sequence
Brucella suis bv. 1
3.5.1.5
14000
-
x * 66000 + x * 15500 + x * 14000, SDS-PAGE
Brucella suis bv. 1
3.5.1.5
15500
-
x * 66000 + x * 15500 + x * 14000, SDS-PAGE
Brucella suis bv. 1
3.5.1.5
61054
-
x * 61054 + x * 11377 + x * 11129, calculated from sequence
Brucella suis bv. 1
3.5.1.5
66000
-
x * 66000 + x * 15500 + x * 14000, SDS-PAGE
Brucella suis bv. 1
3.5.1.5
130000
135000
gel filtration
Brucella suis bv. 1
3.5.1.5
157000
-
nondenaturing PAGE
Brucella suis bv. 1
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.1.5
Brucella suis bv. 1
Q8FZW3
urease subunit beta 2
-
Purification (Commentary)
EC Number
Commentary
Organism
3.5.1.5
-
Brucella suis bv. 1
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.5.1.5
540
-
-
Brucella suis bv. 1
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.1.5
urea + H2O
-
696986
Brucella suis bv. 1
CO2 + 2 NH3
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.5.1.5
?
x * 61054 + x * 11377 + x * 11129, calculated from sequence; x * 66000 + x * 15500 + x * 14000, SDS-PAGE
Brucella suis bv. 1
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.5.1.5
28
35
-
Brucella suis bv. 1
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
3.5.1.5
10
45
-
Brucella suis bv. 1
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.1.5
7.3
-
-
Brucella suis bv. 1
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.5.1.5
6
8
-
Brucella suis bv. 1
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.5.1.5
0.77
-
Acetohydroxamic acid
competitive
Brucella suis bv. 1
3.5.1.5
1.04
-
Hydroxyurea
competitive
Brucella suis bv. 1
3.5.1.5
26.12
-
Thiourea
competitive
Brucella suis bv. 1
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
3.5.1.5
Brucella suis bv. 1
-
-
5
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.1.5
Acetohydroxamic acid
-
Brucella suis bv. 1
3.5.1.5
Hydroxyurea
-
Brucella suis bv. 1
3.5.1.5
Thiourea
-
Brucella suis bv. 1
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.5.1.5
0.77
-
Acetohydroxamic acid
competitive
Brucella suis bv. 1
3.5.1.5
1.04
-
Hydroxyurea
competitive
Brucella suis bv. 1
3.5.1.5
26.12
-
Thiourea
competitive
Brucella suis bv. 1
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.1.5
5.6
-
Urea
by hyperbolic regression, Lineweaver-Burk: 5.24 mM
Brucella suis bv. 1
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.1.5
11129
-
x * 61054 + x * 11377 + x * 11129, calculated from sequence
Brucella suis bv. 1
3.5.1.5
11377
-
x * 61054 + x * 11377 + x * 11129, calculated from sequence
Brucella suis bv. 1
3.5.1.5
14000
-
x * 66000 + x * 15500 + x * 14000, SDS-PAGE
Brucella suis bv. 1
3.5.1.5
15500
-
x * 66000 + x * 15500 + x * 14000, SDS-PAGE
Brucella suis bv. 1
3.5.1.5
61054
-
x * 61054 + x * 11377 + x * 11129, calculated from sequence
Brucella suis bv. 1
3.5.1.5
66000
-
x * 66000 + x * 15500 + x * 14000, SDS-PAGE
Brucella suis bv. 1
3.5.1.5
130000
135000
gel filtration
Brucella suis bv. 1
3.5.1.5
157000
-
nondenaturing PAGE
Brucella suis bv. 1
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.1.5
-
Brucella suis bv. 1
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.5.1.5
540
-
-
Brucella suis bv. 1
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.1.5
urea + H2O
-
696986
Brucella suis bv. 1
CO2 + 2 NH3
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.5.1.5
?
x * 61054 + x * 11377 + x * 11129, calculated from sequence; x * 66000 + x * 15500 + x * 14000, SDS-PAGE
Brucella suis bv. 1
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.5.1.5
28
35
-
Brucella suis bv. 1
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
3.5.1.5
10
45
-
Brucella suis bv. 1
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.1.5
7.3
-
-
Brucella suis bv. 1
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.5.1.5
6
8
-
Brucella suis bv. 1
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
3.5.1.5
Brucella suis bv. 1
-
-
5