Literature summary extracted from

Rost, S.; Pelz, H.J.; Menzel, S.; MacNicoll, A.D.; Leon, V.; Song, K.J.; Jaekel, T.; Oldenburg, J.; Mueller, C.R.
Novel mutations in the VKORC1 gene of wild rats and mice--a response to 50 years of selection pressure by warfarin? (2009), BMC Genet., 10, 4-4.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
1.17.4.4	expressed in HEK-293 cells	Mus musculus

Protein Variants

EC Number	Protein Variants	Comment	Organism
1.17.4.4	A143V	mutant shows about 140% relative VKOR activity as compared to the wild type enzyme	Rattus norvegicus
1.17.4.4	A26S	mutant shows about 70% relative VKOR activity as compared to the wild type enzyme	Mus musculus
1.17.4.4	A26T	mutant shows about 60% relative VKOR activity as compared to the wild type enzyme	Rattus norvegicus
1.17.4.4	A26T	the mutation has only a moderate effect on VKOR activity with a reduction to approximately 56% of wild type activity	Rattus norvegicus
1.17.4.4	A48T	mutant shows about 120% relative VKOR activity as compared to the wild type enzyme	Mus musculus
1.17.4.4	E37G	mutant shows about 75% relative VKOR activity as compared to the wild type enzyme	Mus musculus
1.17.4.4	E67K	the mutant shows a reduced vitamin K epoxide turnover of about 33% compared to the wild type protein, and has no effect on warfarin sensitivity in vitro	Rattus norvegicus
1.17.4.4	F63C	the mutation reduces the VKOR activity to about 30% of normal	Rattus norvegicus
1.17.4.4	I141V	mutant shows about 45% relative VKOR activity as compared to the wild type enzyme	Rattus norvegicus
1.17.4.4	I90L	mutant shows about 90% relative VKOR activity as compared to the wild type enzyme	Rattus norvegicus
1.17.4.4	L128Q	mutant shows moderately reduced VKOR activity (about 60% compared to wild type protein) and is resistant to warfarin inhibition to a variable degree	Rattus norvegicus
1.17.4.4	L128S	mutant shows about 20% relative VKOR activity as compared to the wild type enzyme	Mus musculus
1.17.4.4	R12W	mutant shows about 35% relative VKOR activity as compared to the wild type enzyme	Mus musculus
1.17.4.4	R33P	VKOR activity of the Arg33Pro variant is reduced to 42% of wild type activity	Rattus norvegicus
1.17.4.4	R58G	mutant shows about 40% relative VKOR activity as compared to the wild type enzyme	Mus musculus
1.17.4.4	R61L	mutant shows about 50% relative VKOR activity as compared to the wild type enzyme	Mus musculus
1.17.4.4	W59R	mutant shows 16% residual VKOR activity	Rattus norvegicus
1.17.4.4	Y139C	mutant shows about 30% relative VKOR activity as compared to the wild type enzyme	Mus musculus
1.17.4.4	Y139C	mutant shows moderately reduced VKOR activity (about 60% compared to wild type protein) and is resistant to warfarin inhibition to a variable degree	Rattus norvegicus
1.17.4.4	Y139S	mutant shows moderately reduced VKOR activity and is resistant to warfarin inhibition to a variable degree	Rattus norvegicus
1.17.4.4	Y39N	mutant shows about 30% relative VKOR activity as compared to the wild type enzyme	Rattus norvegicus

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
1.17.4.4	warfarin	almost complete inhibition at 0.06 mM	Mus musculus
1.17.4.4	warfarin	almost complete inhibition at 0.06 mM	Rattus norvegicus

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.17.4.4	Mus musculus	-	-	-
1.17.4.4	Rattus norvegicus	-	-	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.17.4.4	vitamin K 2,3-epoxide + dithiothreitol	-	Mus musculus	vitamin K + oxidized dithiothreitol	-	?
1.17.4.4	vitamin K 2,3-epoxide + dithiothreitol	-	Rattus norvegicus	vitamin K + oxidized dithiothreitol	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
1.17.4.4	Vitamin K reductase	-	Mus musculus
1.17.4.4	Vitamin K reductase	-	Rattus norvegicus
1.17.4.4	vitamin K-epoxide reductase	-	Mus musculus
1.17.4.4	vitamin K-epoxide reductase	-	Rattus norvegicus
1.17.4.4	VKOR	-	Mus musculus
1.17.4.4	VKOR	-	Rattus norvegicus
1.17.4.4	VKORC1	-	Mus musculus
1.17.4.4	VKORC1	-	Rattus norvegicus