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Literature summary extracted from

  • Viator, R.J.; Rest, R.F.; Hildebrandt, E.; McGee, D.J.
    Characterization of Bacillus anthracis arginase: effects of pH, temperature, and cell viability on metal preference (2008), BMC Biochem., 9, 15.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.3.1 expressed in Escherichia coli Bacillus anthracis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.3.1 Co2+ displays remarkable activity in the absence of exogenous metals, although manganese, cobalt, and nickel all improve activity. Enzyme shifts its metal preference from Ni, Co,and Mn (decreasing order) when assayed at pH 6 to Ni, Mn, and Co (decreasing order) at pH 9 Bacillus anthracis
3.5.3.1 Co2+ enzyme is optimally active with cobalt at pH 6 Helicobacter pylori
3.5.3.1 Mn2+ displays remarkable activity in the absence of exogenous metals, although manganese, cobalt, and nickel all improve activity. Enzyme shifts its metal preference from Ni, Co,and Mn (decreasing order) when assayed at pH 6 to Ni, Mn, and Co (decreasing order) at pH 9 Bacillus anthracis
3.5.3.1 additional information Fe2+, Ca2+, Mg2+, Zn2+, Cu2+ have no effect on enzyme activity Bacillus anthracis
3.5.3.1 Ni2+ optimal arginase activity occurrs with nickel at an alkaline pH Bacillus anthracis

Organism

EC Number Organism UniProt Comment Textmining
3.5.3.1 Bacillus anthracis
-
 7702
-
3.5.3.1 Bacillus anthracis 7702
-
 7702
-
3.5.3.1 Helicobacter pylori
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.3.1
-
 Bacillus anthracis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.3.1 additional information
-
 arginase is up to 1000times more active than Helicobacter pylori arginase Bacillus anthracis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.3.1 L-arginine + H2O
-
 Helicobacter pylori L-ornithine + urea
-
 ?
3.5.3.1 L-arginine + H2O
-
 Bacillus anthracis L-ornithine + urea
-
 ?
3.5.3.1 L-arginine + H2O
-
 Bacillus anthracis 7702 L-ornithine + urea
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.3.1 arginase
-
 Helicobacter pylori
3.5.3.1 arginase
-
 Bacillus anthracis
3.5.3.1 RocF
-
 Helicobacter pylori
3.5.3.1 RocF
-
 Bacillus anthracis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.3.1 50 55 activity increased about 10fold following treatment with heat (50-55°C, 30 min) at pH 9.0 in the absence of metal versus no heat, in the presence of various metals, heat treatment does not affect arginase activity at either pH 6.0 or 9.0, when compared to samples that are not heat-treated Bacillus anthracis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.3.1 6
-
-
 Helicobacter pylori
3.5.3.1 6
-
 Escherichia coli (pBS-barocF) has optimal arginase activity at both pH 6.0 and 9.0 with nickel, with lower activity in the presence of cobalt or manganese Bacillus anthracis
3.5.3.1 9
-
 Escherichia coli (pBS-barocF) has optimal arginase activity at both pH 6.0 and 9.0 with nickel, with lower activity in the presence of cobalt or manganese Bacillus anthracis