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Literature summary extracted from

  • Tie, J.; Wu, S.; Jin, D.; Nicchitta, C.V.; Stafford, D.W.
    A topological study of the human gamma-glutamyl carboxylase (2000), Blood, 96, 973-978.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.90 expressed in 293 cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
4.1.1.90 additional information 38-BamHI site introduces 2 amino acid residues (glycine and serine) between the hGC fragment and the Lep tag. A 10-amino acid peptide (MDYKDDDDKG), including the FLAG epitope, is introduced to the amino-terminus of the full length of hGC to make FLAG-hGC and a 8-amino acid peptide (DYKDDDDK) is attached to the carboxyl-terminus of the full length of hGC to make hGC-FLAG. The FLAG-tagged hGC cDNA is subcloned into the EcoRI (Escherichia coli RY13) site of the expression vector pCl-neo under control of the cytomegalovirus (CMV) promoter Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.1.1.90 cytoplasm human glutamyl carboxylase spans the membrane at least 5times, with its N-terminus in the cytoplasm and its C-terminus in the lumen of the endoplasmic reticulum Homo sapiens 5737
-
4.1.1.90 endoplasmic reticulum membrane amino-terminus of the gamma-glutamyl carboxylase is on the cytoplasmic side of the endoplasmic reticulum, while the carboxylterminus is on the lumenal side Homo sapiens 5789
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.90 60000
-
 proteinase K digestion of the hGC-FLAG reveals a 60 kDa fragment, indicating the lumenal location of the FLAG tag and therefore the carboxyl-terminus of the carboxylase. In contrast, FLAG-hGC does not show a proteinase K–resistant fragment except for the residual undigested full-length carboxylase, which indicates the cytoplasmic location of the FLAG tag and therefore the amino-terminus of the carboxylase Homo sapiens
4.1.1.90 95000
-
 full-length carboxylase Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.90 Homo sapiens P38435
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
4.1.1.90 additional information enzyme catalyzes vitamin K-dependent posttranslational modification of glutamate to gamma-carboxyl glutamate Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.90 L-glutamate + CO2 + O2 + vitamin K hydroquinone
-
 Homo sapiens gamma-carboxy L-glutamate + vitamin K epoxide + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.1.90 gamma-glutamyl carboxylase polytopic membrane protein Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.90 vitamin K
-
 Homo sapiens