EC Number | Application | Comment | Organism |
---|---|---|---|
2.2.1.1 | synthesis | desing of an enzyme microreaktor by reversible immobilization of His6-tagged enzyme a 200-microm ID fused silica capillary for quantitative kinetic analysis. Transketolase kinetic parameters in the mircoreactor are comparable with those measured in free solution. Quantitative elution of the immobilized transketolase and the regeneration and reuse of the derivatized capillary over five cycles are possible | Escherichia coli |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.2.1.1 | expression in Escherichia coli, His-taggged protein | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.2.1.1 | 26 | - |
lithium beta-hydroxypyruvate | pH 7.0, 25°C | Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.2.1.1 | Escherichia coli | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.2.1.1 | lithium beta-hydroxypyruvate + glycolaldehyde | - |
Escherichia coli | L-erythrulose + ? | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.2.1.1 | 410000 | - |
lithium beta-hydroxypyruvate | pH 7.0, 25°C | Escherichia coli |