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Literature summary extracted from
- Design of a cytochrome P450BM3 reaction system linked by two-step cofactor regeneration catalyzed by a soluble transhydrogenase and glycerol dehydrogenase (2009), Biotechnol. Prog., 25, 1372-1378.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.1.1.6 | His-tag, expressed in E. coli JM109 | Escherichia coli |
| 1.1.1.6 | His-tagged version | Escherichia coli |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.6.1.1 | soluble | - |
Escherichia coli | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.6 | 40000 | - |
x * 40000, SDS-PAGE | Escherichia coli |
| 1.6.1.1 | 53000 | - |
SDS-PAGE | Escherichia coli |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.6 | Escherichia coli | - |
- |
- |
| 1.1.1.6 | Escherichia coli JM109 | - |
- |
- |
| 1.6.1.1 | Escherichia coli | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.1.6 | - |
Escherichia coli |
| 1.6.1.1 | - |
Escherichia coli |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.6 | 1.26 | - |
- |
Escherichia coli |
| 1.1.1.6 | 1.26 | - |
pH 8.2, 25°C | Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.6 | glycerol + NAD+ | - |
Escherichia coli | dihydroxyacetone + NADH + H+ | - |
? |  |
| 1.1.1.6 | glycerol + NAD+ | - |
Escherichia coli JM109 | dihydroxyacetone + NADH + H+ | - |
? | |
| 1.1.1.6 | glycerol + NAD+ | - |
Escherichia coli | glycerone + NADH + H+ | - |
? | |
| 1.1.1.6 | glycerol + NAD+ | - |
Escherichia coli JM109 | glycerone + NADH + H+ | - |
? | |
| 1.6.1.1 | NADH + thio-NADP+ | - |
Escherichia coli | NAD+ + thio-NADPH | - |
r | |
| 1.6.1.1 | NADH + thio-NADP+ | - |
Escherichia coli JM109 | NAD+ + thio-NADPH | - |
r | |
| 1.6.1.1 | NADP+ + NADH | - |
Escherichia coli | NADPH + NAD+ | - |
r | |
| 1.6.1.1 | NADP+ + NADH | - |
Escherichia coli JM109 | NADPH + NAD+ | - |
r | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.1.1.6 | ? | x * 40000, SDS-PAGE | Escherichia coli |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.1.1.6 | glycerol dehydrogenase | - |
Escherichia coli |
| 1.6.1.1 | soluble pyridine nucleotide transhydrogenase | - |
Escherichia coli |
| 1.6.1.1 | soluble transhydrogenase | - |
Escherichia coli |
| 1.6.1.1 | STH | - |
Escherichia coli |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.6 | 9.5 | 10 | - |
Escherichia coli |
| 1.6.1.1 | 7 | - |
around pH 7.0 | Escherichia coli |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.6 | 6.5 | 11 | - |
Escherichia coli |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.6 | NAD+ | - |
Escherichia coli | |
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Release 2023.1 (January 2023)
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