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Literature summary extracted from

  • Kim, N.; Kim, Y.; Kim, H.; Oh, D.
    Optimized formation of detergent micelles of beta-carotene and retinal production using recombinant human beta, beta-carotene 15,15-monooxygenase (2008), Biotechnol. Prog., 24, 227-231.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.63 into the vector pET-24b+ for expression in Escherichia coli ER2566 cells Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.63 63000
-
 determined by SDS-PAGE Homo sapiens
1.13.11.63 63400
-
 theoretical, His-tagged protein Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.63 beta-carotene + O2 Homo sapiens
-
 retinal
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.63 Homo sapiens Q9HAY6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.63 using a His-Trap HP affinity chromatography column Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.63 1.97
-
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.63 beta-carotene + O2
-
 Homo sapiens retinal
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.63 beta,beta-carotene 15,15'-monooxygenase
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.63 37
-
 activity assay Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.63 8
-
 activity assay Homo sapiens