Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kim, Y.S.; Oh, D.K.
    Substrate specificity of a recombinant chicken beta-carotene 15,15-monooxygenase that converts beta-carotene into retinal (2009), Biotechnol. Lett., 31, 403-408.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.63 into the vector pET-24a+ for expression in Escherichia coli ER2566 cells Gallus gallus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.63 0.026
-
 beta-carotene
-
 Gallus gallus
1.13.11.63 0.18
-
 beta-apo-4'carotenal
-
 Gallus gallus
1.13.11.63 0.19
-
 beta-apo-8'carotenal
-
 Gallus gallus
1.13.11.63 0.29
-
 beta-cryptoxanthin
-
 Gallus gallus
1.13.11.63 0.37
-
 alpha-carotene
-
 Gallus gallus
1.13.11.63 0.69
-
 gamma-carotene
-
 Gallus gallus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.63 60000
-
 determined by SDS-PAGE Gallus gallus
1.13.11.63 61210
-
 theoretical, plus hexahistidine tag Gallus gallus
1.13.11.63 240000
-
 determined by gel filtration Gallus gallus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.63 beta-carotene + O2 Gallus gallus two molecules retinal are formed retinal
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.63 Gallus gallus Q9I993
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.63 by His-Trap HP affinity and Resource Q ion exchange chromatography Gallus gallus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.11.63 liver
-
 Gallus gallus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.63 0.01
-
 crude extract Gallus gallus
1.13.11.63 0.16
-
 purification step His-Trap HP Gallus gallus
1.13.11.63 0.32
-
 purification step Resource Q Gallus gallus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.63 alpha-carotene + O2 one molecule retinal is formed Gallus gallus retinal + alpha-retinal
-
 ?
1.13.11.63 beta-apo-4'-carotenal + O2 one molecule retinal is formed Gallus gallus retinal + ?
-
 ?
1.13.11.63 beta-apo-8'-carotenal + O2 one molecule retinal is formed Gallus gallus retinal + ?
-
 ?
1.13.11.63 beta-carotene + O2 two molecules retinal are formed Gallus gallus retinal
-
 ?
1.13.11.63 beta-cryptoxanthin + O2
-
 Gallus gallus retinal + (3R)-3-hydroxyretinal
-
 ?
1.13.11.63 gamma-carotene + O2
-
 Gallus gallus retinal + acycloretinal one molecule retinal is formed ?

Subunits

EC Number Subunits Comment Organism
1.13.11.63 homotetramer 4 * 60000 Gallus gallus

Synonyms

EC Number Synonyms Comment Organism
1.13.11.63 beta-carotene 15,15'-monooxygenase
-
 Gallus gallus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.63 37
-
 activity assay Gallus gallus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.63 0.0082
-
 gamma-carotene
-
 Gallus gallus
1.13.11.63 0.0083
-
 alpha-carotene
-
 Gallus gallus
1.13.11.63 0.015
-
 beta-apo-4'-carotenal
-
 Gallus gallus
1.13.11.63 0.0183
-
 beta-apo-8'-carotenal
-
 Gallus gallus
1.13.11.63 0.0275
-
 beta-carotene
-
 Gallus gallus
1.13.11.63 0.0333
-
 beta-cryptoxanthin
-
 Gallus gallus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.63 8
-
 activity assay Gallus gallus