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Literature summary extracted from

  • Liu, B.; Wu, L.; Liu, T.; Hong, Y.; Shen, Y.; Ni, J.
    A MOFRL family glycerate kinase from the thermophilic crenarchaeon, Sulfolobus tokodaii, with unique enzymatic properties (2009), Biotechnol. Lett., 31, 1937-1941.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.165 expressed in Escherichia coli Sulfurisphaera tokodaii
2.7.1.165 expressed in Escherichia coli BL21(DE3) cells Sulfurisphaera tokodaii

General Stability

EC Number General Stability Organism
2.7.1.165 resistant to the 1 mM guanidine hydrochloride (85% of the original activity) and 1 mM NaF (65% of original activity) relatively stable in 10 mM MnCl2, CoSO4, CaCl2, SrCl2, NiCl2, CuCl2, HgCl2 Sulfurisphaera tokodaii

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.165 2-propanol 10% v/v, less than 65% inhibition; less than 65% activity in the presence of 10% (v/v) Sulfurisphaera tokodaii
2.7.1.165 Ca2+ 85% activity in the presence of 10 mM Ca2+ compared to Mg2+ Sulfurisphaera tokodaii
2.7.1.165 ethanol 10% v/v, less than 65% inhibition; less than 65% activity in the presence of 10% (v/v) Sulfurisphaera tokodaii
2.7.1.165 guanidine hydrochloride 85% residual activity at 1 M guanidine hydrochloride Sulfurisphaera tokodaii
2.7.1.165 Mn2+ 93% activity in the presence of 10 mM Mn2+ compared to Mg2+ Sulfurisphaera tokodaii
2.7.1.165 n-butanol 10% v/v, less than 65% inhibition; less than 65% activity in the presence of 10% (v/v) Sulfurisphaera tokodaii
2.7.1.165 Ni2+ 68% activity in the presence of 10 mM Ni2+ compared to Mg2+ Sulfurisphaera tokodaii
2.7.1.165 Phenylmethylsulfonylfluoride less than 30% residual activity at 10 mM Sulfurisphaera tokodaii
2.7.1.165 PMSF 10 mM, more than 70% inhibition Sulfurisphaera tokodaii
2.7.1.165 SDS 10 mM, more than 60% inhibition; less than 40% residual activity at 10 mM Sulfurisphaera tokodaii
2.7.1.165 Sodium fluoride 65% residual activity at 1 M sodium fluoride Sulfurisphaera tokodaii
2.7.1.165 Sr2+ 79% activity in the presence of 10 mM Sr2+ compared to Mg2+ Sulfurisphaera tokodaii
2.7.1.165 Zn2+ 84% activity in the presence of 10 mM Zn2+ compared to Mg2+ Sulfurisphaera tokodaii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.165 Ca2+ divalent metal ion required, Ca2+ (10 mM) shows 85% of the activity compared to Mg2+ Sulfurisphaera tokodaii
2.7.1.165 Co2+ divalent metal ion required, maximal activity in presence of Co2+ or Mg2+ (10 mM) Sulfurisphaera tokodaii
2.7.1.165 Co2+ 106% activity in the presence of 10 mM Co2+ compared to Mg2+ Sulfurisphaera tokodaii
2.7.1.165 Mg2+ divalent metal ion required, maximal activity in presence of Co2+ or Mg2+ (10 mM) Sulfurisphaera tokodaii
2.7.1.165 Mg2+ 100% activity in the presence of 10 mM Sulfurisphaera tokodaii
2.7.1.165 Mn2+ divalent metal ion required, Mn2+ (10 mM) shows 93% of the activity compared to Mg2+ Sulfurisphaera tokodaii
2.7.1.165 additional information monovalent metal ions have no effect on the enzyme activity Sulfurisphaera tokodaii
2.7.1.165 Ni2+ divalent metal ion required, Ni2+ (10 mM) shows 68% of the activity compared to Mg2+ Sulfurisphaera tokodaii
2.7.1.165 Sr2+ divalent metal ion required, Sr2+ (10 mM) shows 79% of the activity compared to Mg2+ Sulfurisphaera tokodaii
2.7.1.165 Zn2+ divalent metal ion required, Zn2+ (10 mM) shows 84% of the activity compared to Mg2+ Sulfurisphaera tokodaii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.165 45000
-
2 * 45000, SDS-PAGE Sulfurisphaera tokodaii
2.7.1.165 80000
-
gel filtration Sulfurisphaera tokodaii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.165 ATP + (R)-glycerate Sulfurisphaera tokodaii key enzyme in the Entner-Doudoroff pathway in archaea ADP + 2-phospho-(R)-glycerate
-
?
2.7.1.165 ATP + (R)-glycerate Sulfurisphaera tokodaii 7 key enzyme in the Entner-Doudoroff pathway in archaea ADP + 2-phospho-(R)-glycerate
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.165 Sulfurisphaera tokodaii Q96YZ3
-
-
2.7.1.165 Sulfurisphaera tokodaii 7 Q96YZ3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.165 Ni-NTA column chromatography Sulfurisphaera tokodaii
2.7.1.165 recombinant enzyme Sulfurisphaera tokodaii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.165 ADP + (R)-glycerate 76% of the activity with GTP Sulfurisphaera tokodaii AMP + 2-phospho-(R)-glycerate
-
?
2.7.1.165 ADP + (R)-glycerate 76% of the activity with GTP Sulfurisphaera tokodaii 7 AMP + 2-phospho-(R)-glycerate
-
?
2.7.1.165 ADP + D-glycerate 76% activity compared to GTP Sulfurisphaera tokodaii AMP + 2-phospho-D-glycerate
-
?
2.7.1.165 AMP + (R)-glycerate 54% of the activity with GTP Sulfurisphaera tokodaii adenosine + 2-phospho-(R)-glycerate
-
?
2.7.1.165 AMP + D-glycerate 54% activity compared to GTP Sulfurisphaera tokodaii adenosine + 2-phospho-D-glycerate
-
?
2.7.1.165 ATP + (R)-glycerate key enzyme in the Entner-Doudoroff pathway in archaea Sulfurisphaera tokodaii ADP + 2-phospho-(R)-glycerate
-
?
2.7.1.165 ATP + (R)-glycerate 92% of the activity with GTP Sulfurisphaera tokodaii ADP + 2-phospho-(R)-glycerate
-
?
2.7.1.165 ATP + (R)-glycerate key enzyme in the Entner-Doudoroff pathway in archaea Sulfurisphaera tokodaii 7 ADP + 2-phospho-(R)-glycerate
-
?
2.7.1.165 ATP + (R)-glycerate 92% of the activity with GTP Sulfurisphaera tokodaii 7 ADP + 2-phospho-(R)-glycerate
-
?
2.7.1.165 ATP + D-glycerate 92% activity compared to GTP Sulfurisphaera tokodaii ADP + 2-phospho-D-glycerate
-
?
2.7.1.165 CTP + (R)-glycerate 87% of the activity with GTP Sulfurisphaera tokodaii CDP + 2-phospho-(R)-glycerate
-
?
2.7.1.165 CTP + (R)-glycerate 87% of the activity with GTP Sulfurisphaera tokodaii 7 CDP + 2-phospho-(R)-glycerate
-
?
2.7.1.165 CTP + D-glycerate 87% activity compared to GTP Sulfurisphaera tokodaii CDP + 2-phospho-D-glycerate
-
?
2.7.1.165 diphosphate + (R)-glycerate 63% of the activity with ATP Sulfurisphaera tokodaii phosphate + 2-phospho-(R)-glycerate
-
?
2.7.1.165 diphosphate + (R)-glycerate 63% of the activity with ATP Sulfurisphaera tokodaii 7 phosphate + 2-phospho-(R)-glycerate
-
?
2.7.1.165 diphosphate + D-glycerate 63% activity compared to GTP Sulfurisphaera tokodaii phosphate + 2-phospho-D-glycerate
-
?
2.7.1.165 GTP + (R)-glycerate
-
Sulfurisphaera tokodaii GDP + 2-phospho-(R)-glycerate
-
?
2.7.1.165 GTP + D-glycerate 100% activity towards GTP Sulfurisphaera tokodaii GDP + 2-phospho-D-glycerate
-
?
2.7.1.165 UTP + (R)-glycerate 81% of the activity with GTP Sulfurisphaera tokodaii UDP + 2-phospho-(R)-glycerate
-
?
2.7.1.165 UTP + D-glycerate 81% activity compared to GTP Sulfurisphaera tokodaii UDP + 2-phospho-D-glycerate
-
?

Subunits

EC Number Subunits Comment Organism
2.7.1.165 homodimer 2 * 45000, SDS-PAGE Sulfurisphaera tokodaii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.165 90
-
-
Sulfurisphaera tokodaii

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.1.165 70 100
-
Sulfurisphaera tokodaii
2.7.1.165 70 100 70°C: about 60% of maximal activity, 100°C: about 85% of maximal activity Sulfurisphaera tokodaii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.165 100
-
12 h, more than 85% of the activity remains Sulfurisphaera tokodaii
2.7.1.165 100
-
the enzyme activity shows no obvious decrease even after heat-treatment at 100°C for 12 h and most of the optimum activity (more than 85%) remains Sulfurisphaera tokodaii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.165 4.5
-
-
Sulfurisphaera tokodaii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.165 3 9 pH 3: about 85% of maximal activity, pH 9.0: about 50% of maximal activity Sulfurisphaera tokodaii

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.165 ATP
-
Sulfurisphaera tokodaii