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Literature summary extracted from

  • Dhananjay, S.K.; Mulimani, V.H.
    Purification of alpha-galactosidase and invertase by three-phase partitioning from crude extract of Aspergillus oryzae (2008), Biotechnol. Lett., 30, 1565-1569.
    View publication on PubMed

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.22 Aspergillus oryzae
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Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.22 separation and purification technique for both alpha-galactosidase and invertase simply using ammoniumsulphate and tert-butanol under different conditions. alpha-Galactosidase and invertase are purified 15- and 12-fold with 50 and 54% activity recovery, respectively. Method is non-chromatographic and economical for protein purification Aspergillus oryzae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.22 4-nitrophenyl-alpha-D-galactopyranoside + H2O
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Aspergillus oryzae 4-nitrophenol + D-galactopyranose
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