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Literature summary extracted from

  • Gonzalez-Pombo, P.; Perez, G.; Carrau, F.; Guisan, J.M.; Batista-Viera, F.; Brena, B.M.
    One-step purification and characterization of an intracellular beta-glucosidase from Metschnikowia pulcherrima (2008), Biotechnol. Lett., 30, 1469-1475.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.21 ethanol up to 10% v/v, increase in activity to 120% Metschnikowia pulcherrima
3.2.1.21 Fe3+ 10 mM, 115% of initial activity Metschnikowia pulcherrima

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.21 D-glucose enzyme is active and stable in presence of 5 g glucose per l. In presence of 100 g glucose per l, 25% residual activity Metschnikowia pulcherrima
3.2.1.21 Mg2+ 10 mM, 22% inhibition Metschnikowia pulcherrima
3.2.1.21 additional information not inhibitory: EDTA Metschnikowia pulcherrima
3.2.1.21 Zn2+ 10 mM, complete inhibition Metschnikowia pulcherrima

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.21 1.5
-
 4-nitrophenyl beta-D-glucopyranoside pH 4.5, 23°C Metschnikowia pulcherrima

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.21 49000
-
 x * 49000, SDS-PAGE Metschnikowia pulcherrima
3.2.1.21 400000
-
 gel filtration Metschnikowia pulcherrima

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.2.1.21 Ethanol enzyme is active and stable in presence of 12% v/v ethanol and 5 g per l glucose. Up to 10% ethanol, increase in activity to 120% Metschnikowia pulcherrima

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 Metschnikowia pulcherrima
-
 best source of beta-glucosidase activity among 60 yeasts isolated from grape musts
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.21
-
 Metschnikowia pulcherrima

Storage Stability

EC Number Storage Stability Organism
3.2.1.21 23°C, pH 4.5-7.0, half-life 5 h Metschnikowia pulcherrima
3.2.1.21 4°C, pH 4.5-7.0, half-life 50 h Metschnikowia pulcherrima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Metschnikowia pulcherrima 2-nitrophenol + beta-D-galactose 2% of the activity with 4-nitrophenyl beta-D-glucopyranoside ?
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Metschnikowia pulcherrima 4-nitrophenol + beta-D-glucose
-
 ?
3.2.1.21 cellobiose + H2O
-
 Metschnikowia pulcherrima 2 beta-D-glucose 22% of the activity with 4-nitrophenyl beta-D-glucopyranoside ?
3.2.1.21 additional information enzyme is active against (1-4)beta and (1-2)beta glycosidic linkages Metschnikowia pulcherrima ?
-
 ?
3.2.1.21 saccharose + H2O
-
 Metschnikowia pulcherrima D-glucose + D-fructose 5% of the activity with 4-nitrophenyl beta-D-glucopyranoside ?

Subunits

EC Number Subunits Comment Organism
3.2.1.21 oligomer x * 49000, SDS-PAGE Metschnikowia pulcherrima

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.21 50
-
-
 Metschnikowia pulcherrima

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.21 4
-
 pH 4.5-7.0, half-life 50 h Metschnikowia pulcherrima
3.2.1.21 23
-
 pH 4.5-7.0, half-life 5 h Metschnikowia pulcherrima

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.21 4.5
-
-
 Metschnikowia pulcherrima

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.21 4
-
 unstable below Metschnikowia pulcherrima

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.21 Metschnikowia pulcherrima isoelectric focusing
-
 4.2