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Literature summary extracted from

  • Spadiut, O.; Radakovits, K.; Pisanelli, I.; Salaheddin, C.; Yamabhai, M.; Tan, T.C.; Divne, C.; Haltrich, D.
    A thermostable triple mutant of pyranose 2-oxidase from Trametes multicolor with improved properties for biotechnological applications (2009), Biotechnol. J., 4, 525-534.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.1.3.10 T169G/E542K/V546C mutant, analysis of kinetic parameters and thermal stability Trametes ochracea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.10 0.093
-
 D-galactose T169G/E542K/V546C, mutant, 30°C, substrate D-galactose (1,4-benzoquinone saturated), pH 6.5, standard 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) assay Trametes ochracea
1.1.3.10 0.19
-
 D-galactose T169G/E542K/V546C, mutant, 50°C, substrate D-galactose (1,4-benzoquinone saturated), pH 6.5, standard 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) assay Trametes ochracea
1.1.3.10 0.22
-
 D-glucose T169G/E542K/V546C, mutant, 30°C, substrate D-glucose (1,4-benzoquinone saturated), pH 6.5, standard 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) assay Trametes ochracea
1.1.3.10 0.23
-
 D-galactose wild-type, mutant, 50°C, substrate D-galactose (1,4-benzoquinone saturated), pH 6.5, standard 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) assay Trametes ochracea
1.1.3.10 0.25
-
 D-galactose wild-type, mutant, 30°C, substrate D-galactose (1,4-benzoquinone saturated), pH 6.5, standard 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) assay Trametes ochracea
1.1.3.10 0.31
-
 D-glucose T169G/E542K/V546C, mutant, 50°C, substrate D-glucose (1,4-benzoquinone saturated), pH 6.5, standard 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) assay Trametes ochracea
1.1.3.10 0.4
-
 D-glucose wild-type, mutant, 30°C, substrate D-glucose (1,4-benzoquinone saturated), pH 6.5, standard 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) assay Trametes ochracea
1.1.3.10 0.64
-
 D-glucose T169G/E542K/V546C, mutant, 30°C, substrate D-glucose (O2 saturated), pH 6.5, standard 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) assay Trametes ochracea
1.1.3.10 0.76
-
 D-glucose wild-type, mutant, 30°C, substrate D-glucose (O2 saturated), pH 6.5, standard 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) assay Trametes ochracea
1.1.3.10 0.78
-
 D-glucose wild-type, mutant, 50°C, substrate D-glucose (1,4-benzoquinone saturated), pH 6.5, standard 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) assay Trametes ochracea
1.1.3.10 1.15
-
 D-glucose T169G/E542K/V546C, mutant, 50°C, substrate D-glucose (O2 saturated), pH 6.5, standard 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) assay Trametes ochracea
1.1.3.10 1.18
-
 D-glucose wild-type, mutant, 50°C, substrate D-glucose (O2 saturated), pH 6.5, standard 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) assay Trametes ochracea
1.1.3.10 1.66
-
 D-galactose T169G/E542K/V546C, mutant, 30°C, substrate D-galactose (O2 saturated), pH 6.5, standard 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) assay Trametes ochracea
1.1.3.10 2.76
-
 D-galactose T169G/E542K/V546C, mutant, 50°C, substrate D-galactose (O2 saturated), pH 6.5, standard 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) assay Trametes ochracea
1.1.3.10 7.94
-
 D-galactose wild-type, mutant, 30°C, substrate D-galactose (O2 saturated), pH 6.5, standard 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) assay Trametes ochracea
1.1.3.10 14.6
-
 D-galactose wild-type, mutant, 50°C, substrate D-galactose (O2 saturated), pH 6.5, standard 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) assay Trametes ochracea

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.10 Trametes ochracea Q7ZA32
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.10 additional information
-
 kinetic parameters measured at batch conversion experiments of wild-type pyranose oxidase and mutant T169G/E542K/V546C Trametes ochracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.10 D-galactose + 1,4-benzoquinone
-
 Trametes ochracea 2-dehydro-D-galactose + 1,4-hydroquinone
-
 ?
1.1.3.10 D-galactose + O2
-
 Trametes ochracea 2-dehydro-D-galactose + H2O2
-
 ?
1.1.3.10 D-glucose + 1,4-benzoquinone
-
 Trametes ochracea 2-dehydro-D-glucose + 1,4-hydroquinone
-
 ?
1.1.3.10 D-glucose + O2
-
 Trametes ochracea 2-dehydro-D-glucose + H2O2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.3.10 P2Ox
-
 Trametes ochracea
1.1.3.10 pyranose oxidase
-
 Trametes ochracea

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.3.10 60
-
 T169G/E542K/V546C mutant, half life is increased 76fold compared to wild-type Trametes ochracea
1.1.3.10 70
-
 T169G/E542K/V546C mutant, half life is increased 350fold compared to wild-type Trametes ochracea

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.3.10 0.072
-
 D-glucose T169G/E542K/V546C, mutant, 30°C, substrate D-glucose (O2 saturated), pH 6.5, standard 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) assay Trametes ochracea
1.1.3.10 0.27
-
 D-galactose T169G/E542K/V546C, mutant, 30°C, substrate D-galactose (O2 saturated), pH 6.5, standard 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) assay Trametes ochracea
1.1.3.10 0.35
-
 D-glucose T169G/E542K/V546C, mutant, 50°C, substrate D-glucose (O2 saturated), pH 6.5, standard 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) assay Trametes ochracea
1.1.3.10 0.74
-
 D-galactose T169G/E542K/V546C, mutant, 50°C, substrate D-galactose (O2 saturated), pH 6.5, standard 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) assay Trametes ochracea
1.1.3.10 2.1
-
 D-galactose wild-type, mutant, 30°C, substrate D-galactose (O2 saturated), pH 6.5, standard 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) assay Trametes ochracea
1.1.3.10 5.51
-
 D-galactose wild-type, mutant, 50°C, substrate D-galactose (O2 saturated), pH 6.5, standard 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) assay Trametes ochracea
1.1.3.10 6.61
-
 D-galactose wild-type, mutant, 30°C, substrate D-galactose (1,4-benzoquinone saturated), pH 6.5, standard 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) assay Trametes ochracea
1.1.3.10 14.6
-
 D-galactose wild-type, mutant, 50°C, substrate D-galactose (1,4-benzoquinone saturated), pH 6.5, standard 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) assay Trametes ochracea
1.1.3.10 21.16
-
 D-glucose T169G/E542K/V546C, mutant, 30°C, substrate D-glucose (1,4-benzoquinone saturated), pH 6.5, standard 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) assay Trametes ochracea
1.1.3.10 34
-
 D-glucose wild-type, mutant, 30°C, substrate D-glucose (O2 saturated), pH 6.5, standard 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) assay Trametes ochracea
1.1.3.10 58.9
-
 D-glucose wild-type, mutant, 50°C, substrate D-glucose (O2 saturated), pH 6.5, standard 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) assay Trametes ochracea
1.1.3.10 79.3
-
 D-glucose T169G/E542K/V546C, mutant, 50°C, substrate D-glucose (1,4-benzoquinone saturated), pH 6.5, standard 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) assay Trametes ochracea
1.1.3.10 171
-
 D-galactose T169G/E542K/V546C, mutant, 50°C, substrate D-galactose (1,4-benzoquinone saturated), pH 6.5, standard 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) assay Trametes ochracea
1.1.3.10 349
-
 D-glucose wild-type, mutant, 30°C, substrate D-glucose (1,4-benzoquinone saturated), pH 6.5, standard 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) assay Trametes ochracea
1.1.3.10 615
-
 D-glucose wild-type, mutant, 50°C, substrate D-glucose (1,4-benzoquinone saturated), pH 6.5, standard 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) assay Trametes ochracea