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Literature summary extracted from

  • Ramirez, A.R.; Guerra, Y.; Otero, A.; Garcia, B.; Berry, C.; Mendiola, J.; Hernandez-Zanui, A.; Chavez, M.
    Generation of an affinity matrix useful in the purification of natural inhibitors of plasmepsin II, an antimalarial-drug target (2009), Biotechnol. Appl. Biochem., 52, 149-157.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.23.39 extract from Plexaura homomalla black sea rod, extract contains a reversible tight-binding inhibitor Plasmodium falciparum
3.4.23.39 extract from Xestospongia muta giant barrel sponge, extract contains a reversible tight-binding inhibitor Plasmodium falciparum
3.4.23.39 additional information generation of an affinity matrix for purification of natural inhibitors by immobilization of the recombinant proenzyme to N-hydroxysuccinimide-activated Sepharose and the activation of the immobilized enzyme by incubation at pH 4.4 and 37°C. The matrices are useful in the affinity purification of the inhibitory activity detected in marine invertebrates, such as Xestospongia muta, giant barrel sponge, and the gorgonian seafan coral Plexaura homomalla, with increases of 10.2- and 5.9fold in the specific inhibitory activity, respectively Plasmodium falciparum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.23.39 0.0274
-
 Leu-Ser-Phe(NO2)-Nle-Ala-Leu-Ome pH 4.4, 37°C Plasmodium falciparum

Organism

EC Number Organism UniProt Comment Textmining
3.4.23.39 Plasmodium falciparum
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.23.39 Leu-Ser-Phe(NO2)-Nle-Ala-Leu-OMe + H2O
-
 Plasmodium falciparum ?
-
 ?
3.4.23.39 Lys-Glu-Phe-Val-Phe-Phe(NO2)-Ala-Leu-Lys + H2O
-
 Plasmodium falciparum Lys-Glu-Phe-Val-Phe + Phe(NO2)-Ala-Leu-Lys
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.23.39 10.4
-
 Leu-Ser-Phe(NO2)-Nle-Ala-Leu-Ome pH 4.4, 37°C Plasmodium falciparum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.23.39 0.0000019
-
 extract from Plexaura homomalla pH 4.4, 37°C Plasmodium falciparum
3.4.23.39 0.0000464
-
 extract from Xestospongia muta pH 4.4, 37°C Plasmodium falciparum