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Literature summary extracted from

  • Kaneko, S.; Cheng, M.; Murai, H.; Takenaka, S.; Murakami, S.; Aoki, K.
    Purification and characterization of an extracellular laccase from Phlebia radiata strain BP-11-2 that decolorizes fungal melanin (2009), Biosci. Biotechnol. Biochem., 73, 939-942.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.2 Fe2+ complete inhibition at 1 mM Phlebia radiata
1.10.3.2 Fe3+ complete inhibition at 1 mM Phlebia radiata
1.10.3.2 Hg2+ strong inhibition at 1 mM Phlebia radiata

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.10.3.2 extracellular
-
 Phlebia radiata
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.10.3.2 Cu2+
-
 Phlebia radiata
1.10.3.2 additional information the enzyme activity is not affected by 7 mM Mn2+ and 1 mM H2O2, and by 1 mM of Ag+, Ba2+, Ca2+, Mg2+, Zn2+, Co2+, Ni2+ or Cu2+ or by sulfhydryl or chelating reagents Phlebia radiata

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.2 60000
-
 gel filtration Phlebia radiata

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.10.3.2 melanin + O2 Phlebia radiata the enzyme decolorizes fungal melanin from Alternaria alternata strain NBCR 31805 or black hyphae of Cladosporium sp. strain B-1, but not synthetic melanin, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Phlebia radiata
-
 isolated from forest soil
-
1.10.3.2 Phlebia radiata BP-11-2
-
 isolated from forest soil
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.2 native extracellular enzyme 250fold to homogeneity by anion exchange chromatographies and gel filtration Phlebia radiata

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.10.3.2 2.5
-
 purified native enzyme Phlebia radiata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Phlebia radiata ?
-
 ?
1.10.3.2 2,5-xylidine + O2
-
 Phlebia radiata ?
-
 ?
1.10.3.2 2,6-dimethoxyphenol + O2
-
 Phlebia radiata ?
-
 ?
1.10.3.2 2-ethoxyphenol + O2
-
 Phlebia radiata ?
-
 ?
1.10.3.2 3,4-dimethoxybenzyl alcohol + O2
-
 Phlebia radiata ?
-
 ?
1.10.3.2 4-aminophenol + O2
-
 Phlebia radiata ?
-
 ?
1.10.3.2 4-methoxyphenol + O2
-
 Phlebia radiata ?
-
 ?
1.10.3.2 catechol + O2
-
 Phlebia radiata ?
-
 ?
1.10.3.2 catechol + O2
-
 Phlebia radiata BP-11-2 ?
-
 ?
1.10.3.2 guaiacol + O2
-
 Phlebia radiata ?
-
 ?
1.10.3.2 hydroquinone + O2
-
 Phlebia radiata ?
-
 ?
1.10.3.2 hydroquinone + O2
-
 Phlebia radiata BP-11-2 ?
-
 ?
1.10.3.2 L-tyrosine + O2
-
 Phlebia radiata ?
-
 ?
1.10.3.2 melanin + O2
-
 Phlebia radiata ?
-
 ?
1.10.3.2 melanin + O2 the enzyme decolorizes fungal melanin from Alternaria alternata strain NBCR 31805 or black hyphae of Cladosporium sp. strain B-1, but not synthetic melanin, overview Phlebia radiata ?
-
 ?
1.10.3.2 additional information the enzyme decolorizes fungal melanin and several other dye reagents, overview Phlebia radiata ?
-
 ?
1.10.3.2 additional information the enzyme decolorizes fungal melanin and several other dye reagents, overview Phlebia radiata BP-11-2 ?
-
 ?
1.10.3.2 p-cresol + O2
-
 Phlebia radiata ?
-
 ?
1.10.3.2 pyrogallol + O2
-
 Phlebia radiata ?
-
 ?
1.10.3.2 pyrogallol + O2
-
 Phlebia radiata BP-11-2 ?
-
 ?
1.10.3.2 resorcinol + O2
-
 Phlebia radiata ?
-
 ?
1.10.3.2 syringaldazine + O2
-
 Phlebia radiata ?
-
 ?
1.10.3.2 syringaldazine + O2
-
 Phlebia radiata BP-11-2 ?
-
 ?
1.10.3.2 vanillic acid + O2
-
 Phlebia radiata ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.10.3.2 monomer 1 x 61000 SDS-PAGE Phlebia radiata

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.2 70
-
-
 Phlebia radiata

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.10.3.2 70
-
 purified enzyme, 10 min, 70% activity remaining Phlebia radiata
1.10.3.2 85
-
 purified enzyme, 10 min, inactivation Phlebia radiata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 5
-
-
 Phlebia radiata

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.10.3.2 4.5 7 purified enzyme, 4°C, 24 h, stable Phlebia radiata

Expression

EC Number Organism Comment Expression
1.10.3.2 Phlebia radiata the enzyme expression is induced by 2-ethoxyphenol, 2,5-xylidine, 4-methoxyphenol and p-cresol, and by melanin produced in Cladosporium sp. strain B-1 up