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Literature summary extracted from

  • Nakamura, K.; Inoue, M.; Maeda, M.; Nakano, R.; Hosoi, K.; Fujiyama, K.; Kimura, Y.
    Molecular cloning and gene expression analysis of tomato endo-beta-N-acetylglucosaminidase, an endoglycosidase involved in the production of high-mannose type free N-glycans during tomato fruit ripening (2009), Biosci. Biotechnol. Biochem., 73, 461-464.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.96 expressed from vector pET41-b in Escherichia coli BL21 (DE3) Solanum lycopersicum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.96 Solanum lycopersicum
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.96 fruit expression of Endo-LE mRNA does not vary significantly with the tomato ripening process Solanum lycopersicum
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.96 Manalpha1-2Manalpha1-6(Manalpha1-2Manalpha1-3)Manalpha1-6(Manalpha1-2Manalpha1-2Manalpha1-3)Manbeta1-4GlcNAcbeta1-4GlcNAc-PA + H2O
-
 Solanum lycopersicum Manalpha1-2Manalpha1-6(Manalpha1-2Manalpha1-3)Manalpha1-6(Manalpha1-2Manalpha1-2Manalpha1-3)Manbeta1-4GlcNAc + 2-(acetylamino)-2-deoxy-N-pyridin-2-yl-beta-D-glucopyranosylamine
-
 ?
3.2.1.96 Manalpha1-2Manalpha1-6(Manalpha1-3)Manalpha1-6(Manalpha1-2Manalpha1-2Manalpha1-3)Manbeta1-4GlcNAcbeta1-4GlcNAc-PA + H2O
-
 Solanum lycopersicum Manalpha1-2Manalpha1-6(Manalpha1-3)Manalpha1-6(Manalpha1-2Manalpha1-2Manalpha1-3)Manbeta1-4GlcNAc + 2-(acetylamino)-2-deoxy-N-pyridin-2-yl-beta-D-glucopyranosylamine
-
 ?
3.2.1.96 Manalpha1-2Manalpha1-6(Manalpha1-3)Manalpha1-6(Manalpha1-2Manalpha1-3)Manbeta1-4GlcNAcbeta1-4GlcNAc-PA + H2O
-
 Solanum lycopersicum ?
-
 ?
3.2.1.96 Manalpha1-3Manalpha1-6(Manalpha1-2Manalpha1-3)Manbeta1-4GlcNAcbeta1-4GlcNAc-PA + H2O
-
 Solanum lycopersicum ?
-
 ?
3.2.1.96 Manalpha1-6(Manalpha1-3)Manalpha1-6(Manalpha1-2Manalpha1-2Manalpha1-3)Manbeta1-4GlcNAcbeta1-4GlcNAc-PA + H2O
-
 Solanum lycopersicum Manalpha1-6(Manalpha1-3)Manalpha1-6(Manalpha1-2Manalpha1-2Manalpha1-3)Manbeta1-4GlcNAc + 2-(acetylamino)-2-deoxy-N-pyridin-2-yl-beta-D-glucopyranosylamine
-
 ?
3.2.1.96 Manalpha1-6(Manalpha1-3)Manalpha1-6(Manalpha1-2Manalpha1-3)Manbeta1-4GlcNAcbeta1-4GlcNAc-PA + H2O
-
 Solanum lycopersicum ?
-
 ?
3.2.1.96 Manalpha1-6(Manalpha1-3)Manalpha1-6(Manalpha1-3)Manbeta1-4GlcNAcbeta1-4GlcNAc-PA + H2O
-
 Solanum lycopersicum ?
-
 ?
3.2.1.96 additional information recombinant Endo-LE, as well as native Endo-LE, is highly active towards high mannose type N-glycans with the Manalpha1-2Manalpha1-3Manbeta1-4GlcNAcbeta1-4GlcNAc unit Solanum lycopersicum ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.96 endo-beta-N-acetylglucosaminidase
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 Solanum lycopersicum
3.2.1.96 endo-LE
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 Solanum lycopersicum
3.2.1.96 ENGase
-
 Solanum lycopersicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.96 6.5
-
-
 Solanum lycopersicum