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Literature summary extracted from

  • Zucca, P.; Littarru, M.; Rescigno, A.; Sanjust, E.
    Cofactor recycling for selective enzymatic biotransformation of cinnamaldehyde to cinnamyl alcohol (2009), Biosci. Biotechnol. Biochem., 73, 1224-1226.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.1.195 industry cofactor recycling catalytic system reveals both 100% selectivity and aldehyde conversion within 3 h by usage of ADH, which provides an inexpensive overall process Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.195 cinnamaldehyde substrate inhibition mediated by cinnamaldehyde concentrations far from the Km value of 0.46 mM, which does not allow for excessively increasing the starting cinnamaldehyde concentration, since the process yield may be greatly affected Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.195 0.071
-
 NADH
-
 Saccharomyces cerevisiae
1.1.1.195 0.46
-
 cinnamaldehyde
-
 Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.195 Saccharomyces cerevisiae
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.195 2-propanol + NADP+ 2-propanol can be also used in the cofactor recycling catalytic system as co-solvent like ethanol, but at the same time cinnamaldehyde conversion is lower (85%) and cinnamyl alcohol production is accordingly lower Saccharomyces cerevisiae ? + NADPH + H+
-
 ?
1.1.1.195 cinnamaldehyde + NADH + H+ cofactor recycling catalytic system in which ADH reduces cinnamaldehyde to cinnamyl alcohol (oxidizing NADH to NAD+). THe NAD+ produced is then recycled by ADH at expense of ethanol, which acts as co-solvent for both substrate and product, and is present in large excess to force the whole process toward cinnamaldehyde reduction. Also, elimination of the obtained dehydrogenation product acetaldehyde drives cinnamaldehyde reduction toward completion. 1-2 mM is the ideal starting cinnamaldehyde concentration Saccharomyces cerevisiae cinnamyl alcohol + NAD+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.195 ADH
-
 Saccharomyces cerevisiae
1.1.1.195 alcohol dehydrogenase
-
 Saccharomyces cerevisiae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.195 30 40 up to 30°C, retains all initial catalytic activity, only 18% activity is lost at 40°C, at 60°C 100% loss of activity Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.195 6
-
 optimum for cinnamaldehyde reduction, whereas oxidation of ethanol and 2-propanol is best accomplished at pH 9, pH 7 is a satisfactory compromise Saccharomyces cerevisiae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.195 7
-
 no significant activity loss up to 24 h Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.195 NADH
-
 Saccharomyces cerevisiae