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Literature summary extracted from

  • Watanabe, S.; Viet, D.N.; Kaneko, J.; Kamio, Y.; Yoshida, S.
    Cloning, expression, and transglycosylation reaction of Paenibacillus sp. strain W-61 xylanase 1 (2008), Biosci. Biotechnol. Biochem., 72, 951-958.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.8 industry oligoglycosides have excellent properties as both surfactants and biodegradability enhancers, whereby Xyn1 is probably useful for such synthesis, since Xyn1 has high transglycosylation activity Paenibacillus sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.8 into vector pUC119 and transformation of Escherichia coli DH5alpha. Into the NcoI site of pTrc99A and expressed in Escherichia coli BL21 CodonPlusR-RIL Paenibacillus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.8 20280
-
 sequence analysis Paenibacillus sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 Paenibacillus sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.8 by gel filtration to homogeneity with a purification yield of 11.3% Paenibacillus sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.8 130.4
-
 purified enzyme toward oat spelt xylan Paenibacillus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.8 additional information no activity with xylobiose and xylotriose Paenibacillus sp. ?
-
 ?
3.2.1.8 oat spelt xylan + H2O
-
 Paenibacillus sp. ?
-
 ?
3.2.1.8 xyloheptaose + H2O
-
 Paenibacillus sp. xylotriose + ?
-
 ?
3.2.1.8 xylohexaose + H2O
-
 Paenibacillus sp. 2 xylotriose
-
 ?
3.2.1.8 xylopentaose + H2O
-
 Paenibacillus sp. xylobiose + xylotriose + xylotetraose
-
 ?
3.2.1.8 xylotetraose + H2O
-
 Paenibacillus sp. xylobiose + xylotriose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.8 xylanase 1
-
 Paenibacillus sp.
3.2.1.8 Xyn1
-
 Paenibacillus sp.