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Literature summary extracted from

  • Lan, X.; Zhang, X.; Kodaira, R.; Zhou, Z.; Shimosaka, M.
    Gene cloning, expression, and characterization of a second beta-N-acetylglucosaminidase from the chitinolytic bacterium Aeromonas hydrophila strain SUWA-9 (2008), Biosci. Biotechnol. Biochem., 72, 492-498.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.52 additional information nagB is transcribed when SUWA-9 cells are grown in the presence of colloidal chitin, but not glucose Aeromonas hydrophila

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.52 ligated with vector pQE-30 and expressed in Escherichia coli BL21(DE3) Aeromonas hydrophila

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.52 8.57
-
 4-nitrophenyl-N-acetyl-beta-D-glucosaminide
-
 Aeromonas hydrophila
3.2.1.52 11.1
-
 4-nitrophenyl beta-N-acetyl-D-galactosaminide
-
 Aeromonas hydrophila

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.52 69000
-
 x * 69000, SDS-PAGE Aeromonas hydrophila

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.52 Aeromonas hydrophila
-
-
-
3.2.1.52 Aeromonas hydrophila SUWA-9
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.52 by ion exchange and gel filtration, to homogeneity, 3.9fold Aeromonas hydrophila

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.52 4-nitrophenyl N-acetyl-beta-D-galactosaminide + H2O
-
 Aeromonas hydrophila 4-nitrophenol + N-acetyl-beta-D-galactosamine
-
 ?
3.2.1.52 4-nitrophenyl N-acetyl-beta-D-galactosaminide + H2O
-
 Aeromonas hydrophila SUWA-9 4-nitrophenol + N-acetyl-beta-D-galactosamine
-
 ?
3.2.1.52 4-nitrophenyl-diacetylchitobiose + H2O
-
 Aeromonas hydrophila 4-nitrophenol + N-acetyl-beta-D-glucosamine
-
 ?
3.2.1.52 4-nitrophenyl-diacetylchitobiose + H2O
-
 Aeromonas hydrophila SUWA-9 4-nitrophenol + N-acetyl-beta-D-glucosamine
-
 ?
3.2.1.52 4-nitrophenyl-N-acetyl-beta-D-glucosaminide + H2O
-
 Aeromonas hydrophila 4-nitrophenol + N-acetyl-beta-D-glucosamine
-
 ?
3.2.1.52 4-nitrophenyl-N-acetyl-beta-D-glucosaminide + H2O
-
 Aeromonas hydrophila SUWA-9 4-nitrophenol + N-acetyl-beta-D-glucosamine
-
 ?
3.2.1.52 4-nitrophenyl-N-acetylchitotetraose + H2O
-
 Aeromonas hydrophila 4-nitrophenol + N-acetyl-beta-D-glucosamine
-
 ?
3.2.1.52 4-nitrophenyl-N-acetylchitotetraose + H2O
-
 Aeromonas hydrophila SUWA-9 4-nitrophenol + N-acetyl-beta-D-glucosamine
-
 ?
3.2.1.52 4-nitrophenyl-N-acetylchitotriose + H2O
-
 Aeromonas hydrophila 4-nitrophenol + N-acetyl-beta-D-glucosamine
-
 ?
3.2.1.52 additional information does not hydrolyze 4-nitrophenyl-beta-glucoside nor 4-nitrophenyl-beta-galactoside Aeromonas hydrophila ?
-
 ?
3.2.1.52 additional information does not hydrolyze 4-nitrophenyl-beta-glucoside nor 4-nitrophenyl-beta-galactoside Aeromonas hydrophila SUWA-9 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.52 ? x * 69000, SDS-PAGE Aeromonas hydrophila

Synonyms

EC Number Synonyms Comment Organism
3.2.1.52 NAG-B
-
 Aeromonas hydrophila

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.52 30
-
-
 Aeromonas hydrophila

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.52 6
-
-
 Aeromonas hydrophila