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Literature summary extracted from

  • Fujii, T.; Takaya, N.
    Denitrification by the fungus Fusarium oxysporum involves NADH-nitrate reductase (2008), Biosci. Biotechnol. Biochem., 72, 412-420.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.7.1.1 gene niaD, DNA and amino acid sequence determination and analysis, functional complemention of the defective NO3- assimilation by mutant strain M10, expression in strain T1 Fusarium oxysporum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.7.1.1 nitrate + NADH + H+ Fusarium oxysporum the enzyme is involved in the reduction of NO3- to nitrite at the initial step of denitrification nitrite + NAD+ + H2O
-
 ?
1.7.1.1 nitrate + NADH + H+ Fusarium oxysporum JCM 11502 the enzyme is involved in the reduction of NO3- to nitrite at the initial step of denitrification nitrite + NAD+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.7.1.1 Fusarium oxysporum B1B5E3 fragment; strain JCM11502, gene niaD
-
1.7.1.1 Fusarium oxysporum B1B5E4 fragment; strain JCM11502, gene niaD
-
1.7.1.1 Fusarium oxysporum JCM 11502 B1B5E3 fragment; strain JCM11502, gene niaD
-
1.7.1.1 Fusarium oxysporum JCM 11502 B1B5E4 fragment; strain JCM11502, gene niaD
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.1.1 additional information
-
 enzyme activity in cells under different culture conditions, overview Fusarium oxysporum
1.7.1.1 10.5
-
 cells in presence of NO3- and under O2-limitation Fusarium oxysporum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.1.1 nitrate + NADH + H+ the enzyme is involved in the reduction of NO3- to nitrite at the initial step of denitrification Fusarium oxysporum nitrite + NAD+ + H2O
-
 ?
1.7.1.1 nitrate + NADH + H+ the enzyme is involved in the reduction of NO3- to nitrite at the initial step of denitrification Fusarium oxysporum JCM 11502 nitrite + NAD+ + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.7.1.1 NADH-Nar
-
 Fusarium oxysporum
1.7.1.1 NADH-nitrate reductase
-
 Fusarium oxysporum
1.7.1.1 NADH-NO3- reductase
-
 Fusarium oxysporum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.7.1.1 30
-
 assay at Fusarium oxysporum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.1.1 7.2
-
 assay at Fusarium oxysporum

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.1.1 NADH
-
 Fusarium oxysporum