Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Anggraeni, A.A.; Sakka, M.; Kimura, T.; Ratanakhaokchai, K.; Kitaoka, M.; Sakka, K.
    Characterization of Bacillus halodurans alpha-galactosidase Mel4A encoded by the mel4A gene (BH2228) (2008), Biosci. Biotechnol. Biochem., 72, 2459-2462.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.22 dithiothreitol or similar reducing agent, required Halalkalibacterium halodurans
3.2.1.22 NAD+ strong activation Halalkalibacterium halodurans
3.2.1.22 NADP+ slight activation Halalkalibacterium halodurans

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.22 expression in Escherichia coli Halalkalibacterium halodurans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.22 Co2+ strong activation Halalkalibacterium halodurans
3.2.1.22 Fe2+ strong activation Halalkalibacterium halodurans
3.2.1.22 Mn2+ strong activation Halalkalibacterium halodurans
3.2.1.22 Ni2+ moderate activation Halalkalibacterium halodurans
3.2.1.22 Zn2+ moderate activation Halalkalibacterium halodurans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.22 51000
-
 x * 51000, SDS-PAGE Halalkalibacterium halodurans

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.22 Halalkalibacterium halodurans Q9KAQ9
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.22 15.4
-
 pH 7.4, 37°C, presence of NAD+, Mn2+, dithiothreitol Halalkalibacterium halodurans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.22 4-nitrophenyl-alpha-D-galactopyranoside + H2O
-
 Halalkalibacterium halodurans 4-nitrophenol + D-galactopyranose
-
 ?
3.2.1.22 additional information no substrate: galactomannan Halalkalibacterium halodurans ?
-
 ?
3.2.1.22 raffinose + H2O
-
 Halalkalibacterium halodurans sucrose + D-galactose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.22 ? x * 51000, SDS-PAGE Halalkalibacterium halodurans

Synonyms

EC Number Synonyms Comment Organism
3.2.1.22 Mel4A
-
 Halalkalibacterium halodurans