EC Number | Cloned (Comment) | Organism |
---|---|---|
4.2.1.22 | full-length CBS and truncated enzyme containing residues 1-397 are expressed in Escherichia coli BL21 cells | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.2.1.22 | R224A | the mutation decreases CBS activity by approximately 50% | Homo sapiens |
4.2.1.22 | R51A | the mutation decreases CBS activity by approximately 50% | Homo sapiens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.1.22 | Homo sapiens | P35520 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.2.1.22 | GST Trap FF column chromatography and Mono Q column chromatography | Homo sapiens |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
4.2.1.22 | 1.2 | - |
truncated ferrous mutant enzyme R51A, at 37°C and pH 8 | Homo sapiens |
4.2.1.22 | 1.3 | - |
full-length ferrous mutant enzyme R224A, at 37°C and pH 8 | Homo sapiens |
4.2.1.22 | 1.3 | - |
full-length ferrous mutant enzyme R51A, at 37°C and pH 8 | Homo sapiens |
4.2.1.22 | 2.2 | - |
full-length ferric mutant enzyme R224A, at 37°C and pH 8 | Homo sapiens |
4.2.1.22 | 2.2 | - |
full-length ferric mutant enzyme R51A, at 37°C and pH 8 | Homo sapiens |
4.2.1.22 | 2.8 | - |
full-length ferrous wild type enzyme, at 37°C and pH 8 | Homo sapiens |
4.2.1.22 | 3.8 | - |
truncated ferrous mutant enzyme R224A, at 37°C and pH 8 | Homo sapiens |
4.2.1.22 | 4 | - |
truncated ferric mutant enzyme R51A, at 37°C and pH 8 | Homo sapiens |
4.2.1.22 | 5.2 | - |
truncated ferric wild type enzyme, at 37°C and pH 8 | Homo sapiens |
4.2.1.22 | 6 | - |
truncated ferric mutant enzyme R224A, at 37°C and pH 8 | Homo sapiens |
4.2.1.22 | 8 | - |
truncated ferrous wild type enzyme, at 37°C and pH 8 | Homo sapiens |
4.2.1.22 | 10.3 | - |
full-length ferric wild type enzyme, at 37°C and pH 8 | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.22 | L-serine + L-homocysteine | - |
Homo sapiens | L-cystathionine + H2O | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.2.1.22 | CBS | - |
Homo sapiens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.2.1.22 | 8 | - |
- |
Homo sapiens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
4.2.1.22 | heme | the specific activity of CBS improves 1.8-2.8fold under truncation of R51A and R224A when the heme iron is in the ferric state | Homo sapiens | |
4.2.1.22 | pyridoxal 5'-phosphate | - |
Homo sapiens |