EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.165 | 2-mercaptoethanol | 1 mM, 16% inhibition, production of methyl iodide | Pavlova pinguis | |
2.1.1.165 | CN- | 100 mM, complete inhibition, production of methyl iodide | Pavlova pinguis | |
2.1.1.165 | CoCl2 | 1 mM, 18% inhibition, production of methyl iodide | Pavlova pinguis | |
2.1.1.165 | dithiothreitol | 5 mM, 28% inhibition, production of methyl iodide | Pavlova pinguis | |
2.1.1.165 | EDTA | 5 mM, 32% inhibition, production of methyl iodide | Pavlova pinguis | |
2.1.1.165 | HS- | 10 mM, 84% inhibition, production of methyl iodide | Pavlova pinguis | |
2.1.1.165 | MgSO4 | 1 mM, 25% inhibition, production of methyl iodide | Pavlova pinguis | |
2.1.1.165 | monoiodoacetate | 5 mM, 28% inhibition, production of methyl iodide | Pavlova pinguis | |
2.1.1.165 | additional information | activity is not inhibited by high iodide concentrations | Pavlova pinguis | |
2.1.1.165 | NaN3 | 5 mM, 32% inhibition, production of methyl iodide | Pavlova pinguis | |
2.1.1.165 | NiCl2 | 1 mM, 38% inhibition, production of methyl iodide | Pavlova pinguis | |
2.1.1.165 | PCMB | 0.5 mM, 30% inhibition, production of methyl iodide | Pavlova pinguis | |
2.1.1.165 | SCN- | 10 mM, 60% inhibition, production of methyl iodide | Pavlova pinguis | |
2.1.1.165 | ZnCl2 | 1 mM, 14% inhibition, production of methyl iodide | Pavlova pinguis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.1.165 | 0.012 | - |
S-adenosyl-L-methionine | 30°C | Pavlova pinguis | |
2.1.1.165 | 12 | - |
Iodide | 30°C | Pavlova pinguis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.165 | additional information | various metal ions have no significant effect on methyl iodide production, suggesting that the enzyme does not require metal ions | Pavlova pinguis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.1.1.165 | 29000 | - |
gel filtration | Pavlova pinguis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.1.9 | Pavlova pinguis | - |
- |
- |
2.1.1.165 | Pavlova pinguis | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.1.1.165 | - |
Pavlova pinguis |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.1.1.165 | 0.0018 | - |
- |
Pavlova pinguis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.9 | additional information | the bifunctional enzyme also catalyzes the methylation of iodide and to a lesser extent bromide and chloride | Pavlova pinguis | ? | - |
? | |
2.1.1.9 | S-adenosyl-L-methionine + HS- | the enzyme is strictly dependent on S-adenosyl-L-methionine as a methyl donor | Pavlova pinguis | S-adenosyl-L-homocysteine + CH3SH | - |
? | |
2.1.1.165 | additional information | the enzyme also catalyzes the methylation of HS- to methyl mercaptan (EC 2.1.1.9) | Pavlova pinguis | ? | - |
? | |
2.1.1.165 | S-adenosyl-L-methionine + bromide | - |
Pavlova pinguis | S-adenosyl-L-homocysteine + methyl bromide | - |
? | |
2.1.1.165 | S-adenosyl-L-methionine + chloride | - |
Pavlova pinguis | S-adenosyl-L-homocysteine + methyl chloride | - |
? | |
2.1.1.165 | S-adenosyl-L-methionine + iodide | the enzyme is strictly dependent on S-adenosyl-L-methionine as a methyl donor | Pavlova pinguis | S-adenosyl-L-homocysteine + methyl iodide | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.1.1.165 | monomer | 1 * 29000, SDS-PAGE | Pavlova pinguis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.1.1.9 | HMT | - |
Pavlova pinguis |
2.1.1.165 | HMT | - |
Pavlova pinguis |
2.1.1.165 | S-adenosyl-L-methionine: halide ion methyltransferase | - |
Pavlova pinguis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.1.1.165 | 8 | - |
- |
Pavlova pinguis |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.1.1.165 | 5.4 | 10 | pH 5.4: about 50% of maximal activity, pH 10.0: about 80% of maximal activity | Pavlova pinguis |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
2.1.1.165 | Pavlova pinguis | - |
- |
5.3 |