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Literature summary extracted from

  • Ohsawa, N.; Tsujita, M.; Morikawa, S.; Itoh, N.
    Purification and characterization of a monohalomethane-producing enzyme S-adenosyl-L-methionine: halide ion methyltransferase from a marine microalga, Pavlova pinguis (2001), Biosci. Biotechnol. Biochem., 65, 2397-2404.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.165 2-mercaptoethanol 1 mM, 16% inhibition, production of methyl iodide Pavlova pinguis
2.1.1.165 CN- 100 mM, complete inhibition, production of methyl iodide Pavlova pinguis
2.1.1.165 CoCl2 1 mM, 18% inhibition, production of methyl iodide Pavlova pinguis
2.1.1.165 dithiothreitol 5 mM, 28% inhibition, production of methyl iodide Pavlova pinguis
2.1.1.165 EDTA 5 mM, 32% inhibition, production of methyl iodide Pavlova pinguis
2.1.1.165 HS- 10 mM, 84% inhibition, production of methyl iodide Pavlova pinguis
2.1.1.165 MgSO4 1 mM, 25% inhibition, production of methyl iodide Pavlova pinguis
2.1.1.165 monoiodoacetate 5 mM, 28% inhibition, production of methyl iodide Pavlova pinguis
2.1.1.165 additional information activity is not inhibited by high iodide concentrations Pavlova pinguis
2.1.1.165 NaN3 5 mM, 32% inhibition, production of methyl iodide Pavlova pinguis
2.1.1.165 NiCl2 1 mM, 38% inhibition, production of methyl iodide Pavlova pinguis
2.1.1.165 PCMB 0.5 mM, 30% inhibition, production of methyl iodide Pavlova pinguis
2.1.1.165 SCN- 10 mM, 60% inhibition, production of methyl iodide Pavlova pinguis
2.1.1.165 ZnCl2 1 mM, 14% inhibition, production of methyl iodide Pavlova pinguis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.165 0.012
-
S-adenosyl-L-methionine 30°C Pavlova pinguis
2.1.1.165 12
-
Iodide 30°C Pavlova pinguis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.165 additional information various metal ions have no significant effect on methyl iodide production, suggesting that the enzyme does not require metal ions Pavlova pinguis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.165 29000
-
gel filtration Pavlova pinguis

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.9 Pavlova pinguis
-
-
-
2.1.1.165 Pavlova pinguis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.165
-
Pavlova pinguis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.165 0.0018
-
-
Pavlova pinguis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.9 additional information the bifunctional enzyme also catalyzes the methylation of iodide and to a lesser extent bromide and chloride Pavlova pinguis ?
-
?
2.1.1.9 S-adenosyl-L-methionine + HS- the enzyme is strictly dependent on S-adenosyl-L-methionine as a methyl donor Pavlova pinguis S-adenosyl-L-homocysteine + CH3SH
-
?
2.1.1.165 additional information the enzyme also catalyzes the methylation of HS- to methyl mercaptan (EC 2.1.1.9) Pavlova pinguis ?
-
?
2.1.1.165 S-adenosyl-L-methionine + bromide
-
Pavlova pinguis S-adenosyl-L-homocysteine + methyl bromide
-
?
2.1.1.165 S-adenosyl-L-methionine + chloride
-
Pavlova pinguis S-adenosyl-L-homocysteine + methyl chloride
-
?
2.1.1.165 S-adenosyl-L-methionine + iodide the enzyme is strictly dependent on S-adenosyl-L-methionine as a methyl donor Pavlova pinguis S-adenosyl-L-homocysteine + methyl iodide
-
?

Subunits

EC Number Subunits Comment Organism
2.1.1.165 monomer 1 * 29000, SDS-PAGE Pavlova pinguis

Synonyms

EC Number Synonyms Comment Organism
2.1.1.9 HMT
-
Pavlova pinguis
2.1.1.165 HMT
-
Pavlova pinguis
2.1.1.165 S-adenosyl-L-methionine: halide ion methyltransferase
-
Pavlova pinguis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.165 8
-
-
Pavlova pinguis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.1.1.165 5.4 10 pH 5.4: about 50% of maximal activity, pH 10.0: about 80% of maximal activity Pavlova pinguis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.1.1.165 Pavlova pinguis
-
-
5.3