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Literature summary extracted from

  • Ohishi, K.; Yamagishi, M.; Ohta, T.; Motosugi, M.; Izumida, H.; Sano, H.; Adachi, K.; Miwa, T.
    Purification and properties of two deacetylases produced by Vibrio alginolyticus H-8 (1997), Biosci. Biotechnol. Biochem., 61, 1113-1117.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.105 Ag+ 1 mM AgNO3, complete inhibition Vibrio alginolyticus
3.5.1.105 Al3+ 1 mM AlCl3, 30% inhibition Vibrio alginolyticus
3.5.1.105 Cu2+ 1 mM CuCl2, 53% inhibition Vibrio alginolyticus
3.5.1.105 Fe2+ 1 mM FeSO4, 26% inhibition Vibrio alginolyticus
3.5.1.105 Hg2+ 1 mM HgCl2, complete inhibition Vibrio alginolyticus
3.5.1.105 Sn2+ 1 mM SnCl2, 44% inhibition Vibrio alginolyticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.105 48000
-
 x * 48000, SDS-PAGE Vibrio alginolyticus

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.105 Vibrio alginolyticus Q99PX1
-
-
3.5.1.105 Vibrio alginolyticus H-8 Q99PX1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.105
-
 Vibrio alginolyticus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.105 32.2
-
-
 Vibrio alginolyticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.105 GlcNAc-beta-(1-4)-GlcNAc + H2O i.e. 2-(acetylamino)-4-O-[2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl]-2-deoxy-D-glucopyranose. No activity with chitotriose, chitotetraose, chitopentaose and chitohexaose. The 2-acetamido group is completely hydrolyzed within 3 h, no hydrolysis of 2'-acetamide group occurs Vibrio alginolyticus GlcNAc-beta-(1-4)-GlcN + acetate i.e. 4-O-(N-acetyl-beta-D-glucosaminyl)-D-glucosamine ?
3.5.1.105 GlcNAc-beta-(1-4)-GlcNAc + H2O i.e. 2-(acetylamino)-4-O-[2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl]-2-deoxy-D-glucopyranose. No activity with chitotriose, chitotetraose, chitopentaose and chitohexaose. The 2-acetamido group is completely hydrolyzed within 3 h, no hydrolysis of 2'-acetamide group occurs Vibrio alginolyticus H-8 GlcNAc-beta-(1-4)-GlcN + acetate i.e. 4-O-(N-acetyl-beta-D-glucosaminyl)-D-glucosamine ?

Subunits

EC Number Subunits Comment Organism
3.5.1.105 ? x * 48000, SDS-PAGE Vibrio alginolyticus

Synonyms

EC Number Synonyms Comment Organism
3.5.1.105 deacetylase DA1
-
 Vibrio alginolyticus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.105 45
-
-
 Vibrio alginolyticus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.1.105 30 50 about 50% of maximal activity at 30°C and 50°C Vibrio alginolyticus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.105 30
-
 pH 7.5, 30 min, about 10% loss of activity Vibrio alginolyticus
3.5.1.105 50
-
 pH 7.5, 30 min, about 40% loss of activity Vibrio alginolyticus
3.5.1.105 70
-
 pH 7.5, 30 min, about 80% loss of activity Vibrio alginolyticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.105 8.5 9
-
 Vibrio alginolyticus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.105 6.5 11 pH 6.5: about 45% of maximal activity, pH 11: about 55% of maximal activity Vibrio alginolyticus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.1.105 4
-
 37°C, 20 min, about 75% loss of activity Vibrio alginolyticus
3.5.1.105 5
-
 37°C, 20 min, about 40% loss of activity Vibrio alginolyticus
3.5.1.105 7 11 37°C, 20 min, stable Vibrio alginolyticus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.5.1.105 Vibrio alginolyticus isoelectric focusing
-
 3.3