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Literature summary extracted from

  • Joshi, C.V.; Ghormade, V.; Kunde, P.; Kulkarni, P.; Mamgain, H.; Bhat, S.; Paknikar, K.M.; Deshpande, M.V.
    Flocculation of dimorphic yeast Benjaminiella poitrasii is altered by modulation of NAD-glutamate dehydrogenase (2009), Biores. Technol., 101, 1393-1395.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.4.1.2 biotechnology a strategy to control flocculation is investigated using dimorphic yeast, Benjaminiella poitrasii as a model. Parent form of this yeast (Y) exhibit faster flocculation (11.1 min) than the monomorphic yeast form mutant Y-5 (12.6 min). Flocculation of both Y and Y-5 can be altered by supplementing either substrates or inhibitor of NAD-glutamate dehydrogenase (NAD-GDH) in the growth media. The rate of flocculation is promoted by alpha-ketoglutarate or isophthalic acid and decelerated by glutamate with a statistically significant inverse correlation to corresponding NAD-GDH levels. This opens up new possibilities of using NAD-GDH modulating agents to control flocculation in fermentations for easier downstream processing Benjaminiella poitrasii
1.4.1.2 biotechnology the rate of flocculation is promoted by a-ketoglutarate or isophthalic acid and decelerated by glutamate with a statistically significant inverse correlation to corresponding NAD-GDH levels. These interesting findings open up new possibilities of using NAD-GDH modulating agents to control flocculation in fermentations for easier downstream processing Benjaminiella poitrasii

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.2 isophthalic acid
-
 Benjaminiella poitrasii
1.4.1.4 isophthalic acid
-
 Benjaminiella poitrasii

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.2 Benjaminiella poitrasii
-
-
-
1.4.1.4 Benjaminiella poitrasii
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.2 2-oxoglutarate + NADH + NH3
-
 Benjaminiella poitrasii L-glutamate + NAD+ + H2O
-
 ?
1.4.1.2 L-glutamate + H2O + NADP+
-
 Benjaminiella poitrasii 2-oxoglutarate + NH3 + NADPH
-
 ?
1.4.1.4 2-oxoglutarate + NADPH + NH3
-
 Benjaminiella poitrasii L-glutamate + NADP+ + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.4.1.2 NAD-GDH
-
 Benjaminiella poitrasii
1.4.1.2 NAD-glutamate dehydrogenase
-
 Benjaminiella poitrasii
1.4.1.4 NADP-GDH
-
 Benjaminiella poitrasii
1.4.1.4 NADP-glutamate dehydrogenase
-
 Benjaminiella poitrasii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.2 8
-
 assay at Benjaminiella poitrasii
1.4.1.4 8
-
 assay at Benjaminiella poitrasii