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Literature summary extracted from

  • Van Hecke, W.; Salaheddin, C.; Ludwig, R.; Dewulf, J.; Haltrich, D.; Van Langenhove, H.
    Biocatalytic cascade oxidation using laccase for pyranose 2-oxidase regeneration (2009), Biores. Technol., 100, 5566-5573.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.3.10 heterologously expressed in Escherichia coli BL21 (DE3) Trametes ochracea

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.10 Trametes ochracea
-
-
-
1.1.3.10 Trametes ochracea MB 49
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.10 additional information
-
complete oxidation of D-glucose using pyranose oxidase as the synthesizing biocatalyst and laccase for redox mediator regeneration is described quantitatively to increase both stability and productivity Trametes ochracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.10 D-glucose + 1,4-benzoquinone
-
Trametes ochracea 2-dehydro-D-glucose + 1,4-hydroquinone
-
?
1.1.3.10 D-glucose + 1,4-benzoquinone
-
Trametes ochracea MB 49 2-dehydro-D-glucose + 1,4-hydroquinone
-
?
1.1.3.10 D-glucose + 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) cation radical
-
Trametes ochracea 2-dehydro-D-glucose + 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
-
?
1.1.3.10 D-glucose + 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) cation radical
-
Trametes ochracea MB 49 2-dehydro-D-glucose + 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
-
?
1.1.3.10 D-glucose + O2
-
Trametes ochracea 2-dehydro-D-glucose + H2O2
-
?
1.1.3.10 D-glucose + O2
-
Trametes ochracea MB 49 2-dehydro-D-glucose + H2O2
-
?

Synonyms

EC Number Synonyms Comment Organism
1.1.3.10 P2O
-
Trametes ochracea
1.1.3.10 pyranose 2-Oxidase
-
Trametes ochracea
1.1.3.10 pyranose oxidase
-
Trametes ochracea

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.3.10 2.7
-
D-glucose substrate 1,4-benzoquinone (100 mM D-glucose), pH 4.5 Trametes ochracea
1.1.3.10 5.4
-
D-glucose substrate D-glucose (air saturated), pH 6.5 Trametes ochracea
1.1.3.10 7.1
-
D-glucose substrate O2 (100 mM D-glucose), pH 6.5 Trametes ochracea